Grk5 (NM_030829) Rat Tagged ORF Clone
CAT#: RR202951
- TrueORF®
Grk5 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_030829" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Grk5 |
Synonyms | Gprk5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202951 representing NM_030829
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCCGGGAAGGGGGTGGAGGAAAACGCA AAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGTTTCCTCACATCAACCAGTGTGAAGACCTCCGGAG AACCATAGACAGAGATTACTACAGTCTATGTGACAAGCAACCAATTGGGAGACTGCTTTTTCGACAATTC TGTGAAACCAGGCCTGGACTGGAGTGCTACATTCAGTTCCTGGACTTAGTGGCAGAATATGAAATTACTC CAGATGAAAACCTTGGGGCAAAGGGGAAGGAAATAATGACCAAGTACCTCTCTCCAAAGTCCCCAGTCTT CATTGCCCAAGTTGGACAGGACCTGGTCTCCCAGACAGAGAAGAAGCTCCTGCAGAGCCCCTGCAAAGAA CTCTTCTCTGCTTGTGCTCAGTCTGTCCATGACTACTTGAAGGGAGACCCCTTCCACGAGTACCTGGACA GCATGTATTTTGACCGTTTTCTGCAGTGGAAATGGTTAGAAAGACAACCAGTGACCAAAAACACTTTCCG GCAGTACCGAGTGCTGGGCAAAGGGGGCTTTGGAGAGGTTTGTGCCTGCCAGGTTCGGGCCACTGGTAAA ATGTATGCTTGCAAACGCTTAGAGAAGAAGAGGATCAAAAAAAGGAAAGGAGAGTCCATGGCACTCAATG AAAAGCAGATTCTTGAGAAAGTCAACAGCCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAAGA TGCACTGTGCTTGGTTCTGACCATTATGAATGGAGGTGACCTGAAGTTTCACATCTACAACATGGGAAAT CCTGGCTTTGAGGAAGAACGAGCCTTGTTTTATGCAGCTGAGATCCTCTGTGGCCTGGAAGACTTACACC GTGAGAACACTGTCTATAGAGATCTAAAACCAGAAAACATCTTGCTGGATGATTATGGCCACATCAGGAT CTCAGACCTAGGACTGGCTGTGAAGATCCCTGAGGGGGACCTTATCCGTGGCCGGGTAGGCACTGTTGGC TACATGGCCCCAGAAGTTCTGAACAACCAGCGATATGGCCTGAGCCCTGACTACTGGGGCCTGGGCTGCC TCATCTATGAGATGATCGAGGGCCAGTCACCATTCCGAGGCCGCAAGGAGAAGGTGAAGCGGGAAGAGGT GGATCGCCGGGTGCTGGAGACTGAGGAAGTGTATTCCCCCAAGTTCTCTGAAGAGGCCAAGTCCATCTGC AACATGCTGCTCACCAAAGACTCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCCGAGGTCAAGA GGCACCCCTTCTTCAGGAACATGAACTTTAAACGCCTGGAGGCTGGGATGTTGGACCCTCCCTTCGTTCC AGATCCCCGGGCTGTATACTGCAAGGATGTGCTGGACATAGAGCAGTTCTCCACTGTGAAAGGTGTCAAC TTGGACCATACGGACGATGACTTCTACTCAAAGTTCTCCACAGGCTCTGTGCCAATCCCATGGCAAAACG AGATGATAGAAACAGAATGTTTCAAGGAGCTGAATGTGTTCGGACCCAACGGTACCCTCTCACCAGACCT GAACAGAAGTCAGCCTCCAGAACCACCAAAGAAAGGGCTGTTCCACAGACTCTTCAGGCGTCAGCATCAA AACAATTCCAAGAGTTCACCTACTCCTAAGACCAGTTGTAACCACCGCATAAATTCAAACCACATCAATT CAAACTCCACTGGAAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202951 representing NM_030829
Red=Cloning site Green=Tags(s) MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYYSLCDKQPIGRLLFRQF CETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLSPKSPVFIAQVGQDLVSQTEKKLLQSPCKE LFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK MYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGN PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVG YMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSPKFSEEAKSIC NMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVN LDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQ NNSKSSPTPKTSCNHRINSNHINSNSTGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030829 |
ORF Size | 1770 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030829.1, NP_110456.1 |
RefSeq Size | 2318 bp |
RefSeq ORF | 1773 bp |
Locus ID | 59075 |
UniProt ID | Q62833 |
Cytogenetics | 1q55 |
MW | 67.8 kDa |
Gene Summary | plays a role in homologous desentization of the thyrotropin receptor [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202951 | Grk5 (untagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), (10 ug) |
USD 605.00 |
|
RR202951L3 | Lenti ORF clone of Grk5 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), (10 ug) |
USD 904.00 |
|
RR202951L4 | Lenti ORF clone of Grk5 (mGFP-tagged ORF) - Rat G protein-coupled receptor kinase 5 (Grk5), (10 ug) |
USD 904.00 |
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