Htr1f (NM_021857) Rat Tagged ORF Clone
CAT#: RR202575
- TrueORF®
Htr1f (Myc-DDK-tagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 1F (Htr1f), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_021857" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Htr1f |
Synonyms | Htr1eb |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202575 representing NM_021857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCTAAACTCATCAGACCAAAATTTAACCTCAGAGGAACTGTTAAACCGAATGCCATCCAAAA TTCTGGTATCCCTCACTCTGTCTGGCCTGGCCTTGATGACAACCACCATCAACTCCCTCGTGATCACTGC AATCATTGTGACTCGGAAGCTGCACCACCCAGCCAACTATTTAATCTGTTCCTTGGCAGTTACAGACTTT CTTGTTGCTGTCCTGGTTATGCCCTTTAGCATCGTGTACATTGTGAGCGAGAGTTGGATTATGGGACAAG GACTCTGTGACCTTTGGCTGAGTGTTGACATCATCTGCTGTAGCTGCTCCATCTTGCACCTGTCGGCTAT AGCGTTGGATAGGTACCGAGCAATCACAGACGCAGTCGAGTATGCCAGGAAGAGGACTCCCAGGCATGCT GGCATCACGATTACAACAGTGTGGGTTATATCTGTGTTCATCTCCATGCCTCCTCTCTTCTGGAGGCACC AAGGAAATAGCCGTGATGATCAGTGTATCATCAAACATGACCATATTGTTTCCACAATTTACTCCACGTT TGGAGCCTTCTACATCCCACTTGTGTTGATATTGATCCTCTACTACAAAATATACAGAGCAGCAAGGACA CTATACCACAAGAGACAAGCAAGTCGGATGATAAAGGAGGAACTGAATGGCCAAGTCCTTTTGGAGAGTG GTGAGAAGAGCATTAAACTGGTCTCCACATCCTACATGTTAGAAAAATCCTTATCTGATCCATCAACAGA CTTTGATAGAATTCACAGCACAGTGAAAAGTCCCAGATCTGAGCTGAAGCACGAGAAATCTTGGAGAAGA CAGAAAATCTCAGGCACTCGAGAACGCAAAGCAGCCACTACCCTGGGATTGATCTTGGGCGCATTTGTAA TATGTTGGTTGCCCTTTTTTGTAAAGGAATTGGTTGTTAATATCTGTGAAAAATGTAAAATTTCTGAAGA AATGTCAAATTTTTTGGCATGGCTTGGTTACCTGAATTCTCTTATAAACCCACTGATTTATACCATCTTT AATGAAGACTTTAAGAAAGCCTTCCAAAAACTTGTACGATGTCGAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202575 representing NM_021857
Red=Cloning site Green=Tags(s) MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVITAIIVTRKLHHPANYLICSLAVTDF LVAVLVMPFSIVYIVSESWIMGQGLCDLWLSVDIICCSCSILHLSAIALDRYRAITDAVEYARKRTPRHA GITITTVWVISVFISMPPLFWRHQGNSRDDQCIIKHDHIVSTIYSTFGAFYIPLVLILILYYKIYRAART LYHKRQASRMIKEELNGQVLLESGEKSIKLVSTSYMLEKSLSDPSTDFDRIHSTVKSPRSELKHEKSWRR QKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNICEKCKISEEMSNFLAWLGYLNSLINPLIYTIF NEDFKKAFQKLVRCRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021857 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021857.3, NP_068629.2 |
RefSeq Size | 1401 bp |
RefSeq ORF | 1101 bp |
Locus ID | 60448 |
Cytogenetics | 11p12 |
MW | 41.8 kDa |
Gene Summary | G-protein coupled receptor for serotonin [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202575 | Htr1f (untagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 1F (Htr1f), (10 ug) |
USD 503.00 |
|
RR202575L3 | Lenti ORF clone of Htr1f (Myc-DDK-tagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 1F (Htr1f), (10 ug) |
USD 803.00 |
|
RR202575L4 | Lenti ORF clone of Htr1f (mGFP-tagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 1F (Htr1f), (10 ug) |
USD 803.00 |
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