Ppp4r3b (NM_001108367) Rat Tagged ORF Clone

CAT#: RR202324

Smek2 (Myc-DDK-tagged ORF) - Rat SMEK homolog 2, suppressor of mek1 (Dictyostelium) (Smek2), (10 ug)

Product Images

Circular map for RR202324

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp4r3b
• Synonyms: RGD1309450; Smek2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202324 representing NM_001108367
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGATACACGGCGGCGAGTGAAGGTCTATACCCTGAACGAAGACCGGCAATGGGACGACCGAGGCA
  CCGGGCACGTCTCCTCCACTTACGTAGAGGAGCTCAAAGGGATGTCGCTGCTGGTTCGAGCAGAGTCCGA
  CGGATCTCTACTCTTGGAGTCAAAGATAAATCCAAACACTGCATATCAGAAGCAGCAGGATACATTAATT
  GTTTGGTCAGAAGCTGAAAACTATGATTTGGCTCTAAGTTTTCAAGAAAAAGCTGGCTGTGATGAAATTT
  GGGAAAAAATTTGTCAAGTTCAAGGTAAGGACCCATCAGTAGAAGTCACACAGGACCTCATTGATGAATC
  AGAAGAAGAACGATTTGAAGAAATGCCAGAAACTAGTCATCTAATTGACCTGCCCACATGTGAACTCAGT
  AAACTTGAAGAGATTGCTGACTTAGTTACCTCAGTTCTCTCCTCACCTATCCGTAGGGAAAAGCTGGCTC
  TGGCCTTGGAAAATGAAGGCTATATCAAAAAACTGCTGCAGCTGTTCCAGGCTTGTGAGAACCTAGAAAA
  CACTGAAGGCTTACACCAGCTGTATGAGATTATTAGAGGAATCTTATTCCTAAATAAGGCAACTCTTTTT
  GAGGTAATGTTTTCTGATGAGTGTATCATGGATGTCGTGGGATGCCTTGAATATGATCCTGCTTTGGCCC
  AGCCAAAAAGACATAGAGAATTCTTGACTAAAACTGCAAAGTTTAAGGAAGTTATACCAATAACAGACTC
  GGAACTAAGGCAAAAAATACATCAGACTTACAGGGTACAATACATTCAGGACATCATTTTACCTACACCA
  TCTGTTTTTGAAGAGAATTTCCTTTCTACTCTTACATCTTTTATTTTCTTCAACAAAGTTGAAATAGTCA
  GCATGCTGCAGGAAGATGAGAAATTTTTGTCTGAAGTTTTTGCACAATTAACAGATGAGGCTACAGATGA
  TGATAAACGGCGCGAACTGGTTAATTTCTTCAAAGAATTTTGTGCATTTTCTCAGACATTGCAACCTCAG
  AACAGGGATGCTTTTTTCAAAACCTTGGCAAAATTGGGAATTCTTCCAGCTCTTGAAATTGTAATGGGCA
  TGGATGATTTGCAAGTCAGATCAGCTGCTACAGATATATTTTCTTATCTGGTAGAATTTAGTCCATCTAT
  GGTACGAGAATTTGTGATGCAAGAAGCCCAGCAGAGTGATGATGATATACTTCTCATTAACGTGGTGATT
  GAACAAATGATTTGTGATACTGACCCTGAGCTAGGAGGTGCTGTTCAACTAATGGGGCTTCTTCGTACTC
  TAATTGATCCAGAGAACATGTTGGCTACAACTAATAAAACTGAAAAGAGTGAGTTCTTAAATTTCTTCTA
  CAATCATTGTATGCATGTTCTCACAGCTCCACTCTTGACCAATACATCAGAAGACAAATGTGAGAAAGAT
  AATATGCTTGGATCTAACAAAACCAACACAATTTGTCCTGATAATTATCAAACAGCACAGCTACTTGCCT
  TAATTTTAGAATTACTCACATTTTGTGTGGAACATCACACATACCATATAAAAAACTACATCATGAACAA
  GGATTTGCTAAGAAGAGTCTTGGTGTTGATGAATTCAAAGCATACTTTTCTGGCCTTGTGTGCTCTTCGT
  TTTATGAGGCGAATAATTGGCCTTAAGGATGAGTTTTATAATCGTTACATCACCAAAGGAAATCTTTTTG
  AACCAGTTATAAATGCCCTTTTGGATAATGGAACTCGGTACAATCTATTAAATTCAGCTGTTATTGAATT
  GTTTGAATTTATAAGAGTGGAAGATATCAAGTCTCTCACTGCACATATAGTTGAAAACTTTTATAAAGCA
  CTTGAATCGATTGAATATGTTCAGACATTCAAAGGATTGAAGACTAAATATGAACAAGAAAAAGACAGAC
  AAAATCAGAAACTGAACAGTGTGCCATCTATATTGCGTAGTAACCGATTTCGCAGAGATGCAAAAGCCTT
  GGAAGAGGATGAAGAAATGTGGTTTAATGAAGATGATGATGAAGAAGGAAAGTCAGTTGTAACACCAGTT
  GAAAAAACTAAGACTGAAGATGATTTTCCAGATAGTTACGAAAAATTTATGGAGACTAAAAAAGCAAAAG
  AAAGTGAAGACAAGGAAAACCTTCCCAAAAGGACATCTTCTGGTGGCTTCAAATTTACTTTCTCCCATTC
  CCCCAGTGCTGCTAATGGAACAAACAGTACAAACAGTAAGTCTGTAGTGGCTCAGACAACACCAGCAAGT
  TCTAATGGGTCCTCTTCCAAAACTACAAACTTGGCTACATCAGTGACAGCCACTAAGGGAAGTTTGGTTG
  GCTTAGTGGACTATCCAGATGATGAAGAGGAAGATGAGGAAGAAGAATCATCCCCCAGAAAAAGACCTCG
  CCTTGGCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202324 representing NM_001108367
  Red=Cloning site Green=Tags(s)
  
  MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLI
  VWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDLIDESEEERFEEMPETSHLIDLPTCELS
  KLEEIADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQACENLENTEGLHQLYEIIRGILFLNKATLF
  EVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILPTP
  SVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQ
  NRDAFFKTLAKLGILPALEIVMGMDDLQVRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVI
  EQMICDTDPELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKD
  NMLGSNKTNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLVLMNSKHTFLALCALR
  FMRRIIGLKDEFYNRYITKGNLFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKA
  LESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDDDEEGKSVVTPV
  EKTKTEDDFPDSYEKFMETKKAKESEDKENLPKRTSSGGFKFTFSHSPSAANGTNSTNSKSVVAQTTPAS
  SNGSSSKTTNLATSVTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108367
• ORF Size: 2460 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108367.1, NP_001101837.1
• RefSeq Size: 5014 bp
• RefSeq ORF: 2463 bp
• Locus ID: 360993
• Cytogenetics: 14q22
• MW: 94 kDa