Nt5c3a (NM_001107862) Rat Tagged ORF Clone

CAT#: RR201666

Nt5c3 (Myc-DDK-tagged ORF) - Rat 5'-nucleotidase, cytosolic III (Nt5c3), (10 ug)

Product Images

Circular map for RR201666

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nt5c3a
• Synonyms: Nt5c3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201666 representing NM_001107862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAATCAGGAGTCTGCTGTCCGTTTGGAAATGATGCCAGAATTTCAGAAAAGCTCAGTTCGTATCA
  AGAACCCAACGAGAGTAGAAGAAATTATCTGTGGTCTCATTAAAGGAGGCGCTGCCAAACTTCAGATAAT
  AACCGACTTTGATATGACTCTAAGTAGGTTCTCCTACAATGGGAAAAGATGCCCAACATGTCATAATATC
  ATTGACAACTGTAAGCTCGTTACAGATGAATGTCGAAGAAAGTTACTGCAGCTAAAGGAGCAGTACTACG
  CTATTGAGGTTGATCCTGTTCTCACCGTGGAAGAGAAGTTCCCTTACATGGTAGAATGGTATACCAAATC
  GCATGGTTTGCTTATTGAACAAGGCATACCAAAAGCTAAACTTAAAGAGATTGTGGCTGACTCTGATGTC
  ATGCTCAAGGAAGGATATGAGAATTTCTTCGGTAAACTTCAACAGCACGGTATCCCTGTGTTCATATTTT
  CGGCTGGTATCGGTGACGTACTTGAAGAAGTTATCCGGCAGGCTGGAGTTTATCACTCAAACGTCAAAGT
  AGTGTCTAACTTCATGGATTTTGATGAAAATGGAGTGCTGAAAGGATTCAAAGGAGAGCTAATTCATGTA
  TTCAACAAACATGATGGTGCCCTGAAGAATACAGACTACTTCAGCCAACTGAAAGACAACAGCAACATCA
  TTCTGCTGGGGGACTCCCAAGGAGACTTAAGGATGGCCGATGGTGTAGCCAACGTCGAACACATTCTGAA
  AATTGGTTATCTGAATGACAGAGTAGACGAGCTTTTAGAAAAATACATGGACTCTTATGATATTGTTTTA
  GTAAAAGAAGAATCACTGGAGGTTGTCAACTCTATTTTACAGAAGACATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201666 representing NM_001107862
  Red=Cloning site Green=Tags(s)
  
  MTNQESAVRLEMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYNGKRCPTCHNI
  IDNCKLVTDECRRKLLQLKEQYYAIEVDPVLTVEEKFPYMVEWYTKSHGLLIEQGIPKAKLKEIVADSDV
  MLKEGYENFFGKLQQHGIPVFIFSAGIGDVLEEVIRQAGVYHSNVKVVSNFMDFDENGVLKGFKGELIHV
  FNKHDGALKNTDYFSQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVL
  VKEESLEVVNSILQKTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107862
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107862.1, NP_001101332.1
• RefSeq Size: 1617 bp
• RefSeq ORF: 894 bp
• Locus ID: 312373
• Cytogenetics: 4q24
• MW: 33.8 kDa