Kcnn2 (NM_019314) Rat Tagged ORF Clone

CAT#: RR201448

Kcnn2 (Myc-DDK-tagged ORF) - Rat potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (Kcnn2), (10 ug)

Product Images

Circular map for RR201448

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnn2
• Synonyms: KCa2.2; SK2; SKCa 2; SKCa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201448 representing NM_019314
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCTGCAGGTACAACGGGGGCGTCATGCGTCCGCTCAGCAACTTGAGCTCGTCCCGCCGGAACC
  TGCACGAGATGGACTCAGAGGCTCAGCCCCTGCAGCCCCCAGCGTCGGTTGTAGGAGGAGGTGGTGGTGC
  GTCCTCCCCGTCTGCTGCCGCCGCCGCCTCATCCTCAGCCCCAGAGATCGTGGTGTCTAAGCCGGAGCAC
  AACAATTCTAACAACCTGGCGCTCTACGGAACTGGCGGCGGAGGCAGCACCGGAGGCGGCGGCGGCGGCG
  GCGGCGGCGGCGGCGGCAGCGGGCATGGCAGCAGCAGCGGCACTAAGTCCAGCAAAAAGAAGAACCAGAA
  CATCGGCTATAAGCTGGGCCATCGGCGTGCCCTGTTTGAGAAGCGCAAGCGGCTCAGCGACTATGCGCTC
  ATCTTCGGCATGTTCGGCATCGTGGTCATGGTCATCGAGACCGAGCTGTCGTGGGGCGCCTACGACAAGG
  CGTCGCTGTATTCTTTAGCTCTGAAATGCCTTATCAGTCTCTCCACGATCATCCTGCTTGGTCTGATCAT
  CGTATACCACGCCAGGGAAATACAGTTATTCATGGTGGACAATGGAGCAGATGACTGGAGAATAGCCATG
  ACTTATGAACGTATTTTCTTCATCTGCTTGGAAATACTGGTGTGTGCTATTCATCCCATCCCTGGGAATT
  ATACGTTCACATGGACAGCCCGGCTTGCCTTCTCCTATGCCCCTTCCACAACCACTGCAGACGTGGATAT
  TATTTTATCTATACCAATGTTCTTAAGACTCTATCTGATTGCCAGAGTCATGCTATTACATAGCAAACTT
  TTCACCGATGCCTCCTCTAGAAGCATTGGGGCACTTAATAAGATAAACTTCAATACGCGTTTTGTTATGA
  AGACTTTAATGACTATCTGCCCAGGAACTGTGCTCTTGGTTTTTAGTATCTCGTTATGGATAATTGCCGC
  ATGGACTGTCCGAGCTTGTGAAAGGTACCATGATCAACAGGATGTCACTAGCAACTTCCTTGGAGCAATG
  TGGTTGATATCAATAACTTTTCTCTCCATTGGTTATGGTGACATGGTACCTAACACATACTGTGGGAAAG
  GAGTCTGCTTGCTTACCGGAATAATGGGTGCAGGTTGCACAGCCTTGGTGGTAGCCGTAGTGGCAAGGAA
  GCTAGAACTTACCAAAGCAGAAAAGCATGTGCACAATTTCATGATGGATACTCAGCTGACCAAAAGAGTA
  AAAAACGCAGCCGCCAATGTACTCAGGGAAACGTGGTTAATCTACAAAAACACAAAGCTAGTGAAAAAGA
  TCGACCATGCAAAAGTAAGGAAGCATCAACGGAAATTCTTACAAGCTATTCATCAATTAAGAAGTGTGAA
  GATGGAACAGAGGAAACTGAATGACCAAGCGAATACGCTAGTGGATCTGGCAAAGACCCAGAATATCATG
  TATGATATGATTTCCGACTTAAATGAAAGGAGTGAAGACTTTGAGAAAAGGATCGTCACCCTGGAAACAA
  AATTAGAAACTTTGATTGGTAGCATTCATGCCCTCCCTGGGCTTATCAGCCAGACCATCAGACAGCAGCA
  AAGGGACTTCATAGAGACACAGATGGAGAACTATGACAAGCATGTCACCTACAATGCTGAGCGTTCCCGG
  TCCTCGTCCAGGAGGCGGCGGTCCTCCTCCACAGCGCCACCAACTTCATCTGAGAGTAGCTCCTCGTCCA
  GGAGGCGGCGGTCCTCCTCCACAGCGCCACCAACTTCATCTGAGAGTAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201448 representing NM_019314
  Red=Cloning site Green=Tags(s)
  
  MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGGGGASSPSAAAAASSSAPEIVVSKPEH
  NNSNNLALYGTGGGGSTGGGGGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYAL
  IFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAM
  TYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKL
  FTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAM
  WLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRV
  KNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIM
  YDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSR
  SSSRRRRSSSTAPPTSSESSSSSRRRRSSSTAPPTSSESS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019314
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2060 bp
• RefSeq ORF: 1743 bp
• Locus ID: 54262
• UniProt ID: P70604
• Cytogenetics: 18q11
• MW: 63.8 kDa
• Gene Summary: small-conductance, calcium-activated potassium channel [RGD, Feb 2006]