Cx3cl1 (NM_134455) Rat Tagged ORF Clone
CAT#: RR200999
- TrueORF®
Cx3cl1 (Myc-DDK-tagged ORF) - Rat chemokine (C-X3-C motif) ligand 1 (Cx3cl1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_134455" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cx3cl1 |
Synonyms | Cx3c; Scyd1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200999 representing NM_134455
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCTCACAGCTCGCGTGGCTGCTGCGCCTGGCCGCGTTCTTTCATCTGTGTACTCTGCTGGCGG GTCAGCACCTCGGCATGACGAAATGCAACATCACGTGCCACAAGATGACCTCGCCAATCCCAGTGACCTT GCTCATCCACTATCAACTGAACCAGGAGTCCTGCGGCAAGCGCGCCATCATCCTGGAGACGAGACAGCAC AGACACTTCTGTGCTGACCCAAAGGAGAAATGGGTCCAAGACGCCATGAAGCACCTGGACCACCAGACCG CTGCCCTGACTAGAAATGGTGGCAAGTTTGAGAAGCGTGTGGACAATGTGACACCTAGGATCACCTCGGC CACCAGGGGACTGTCCCCAACTGCCCTGGCAAAGCCTGAGTCAGCCACAGTGGAAGACCTTACTTTGGAA CCGACTGCTATTTCCCAGGAGGCCCGAAGGCCCATGGGGACTTCCCAAGAGCCACCAGCAGCAGTGACTG GATCGTCTCCCTCAACTTCCAAAGCTCAGGATGCAGGGCTTGCCGCCAAGCCTCAGAGCACTGGAATTTC TGAGGTGGCTGCTGTCTCTACCACCATTTGGCCGAGTTCTGCTGTCTACCAATCTGGATCTAGCCTCTGG GCCGAGGAAAAAGCTACTGAATCCCCCCCCACTATAGCCCTATCTACTCAAGCGTCCACCACTTCAAGCC CCAAGCAGAATGTTGGGTCTGAAGGCCAGCCCCCATGGGTCCAGGAGCAGGACTCCACTCCAGAGAAATC TCCAGGGCCTGAGGAGACAAACCCAGTTCATACTGATATTTTCCAAGACAGAGGACCTGGCAGCACAGTC CATCCCTCAGTGGCTCCCACCTCCTCTGAAAAGACCCCCAGCCCGGAGCTGGTGGCCTCGGGCAGCCAGG CTCCTAAGGTAGAGGAACCCATTCATGCCACTGCAGATCCCCAGAAACTGAGTGTGTTTATCACTCCTGT CCCTGACTCCCAGGCAGCCACCCGGAGGCAGGCAGTGGGGTTACTGGCCTTTCTTGGTCTACTCTTCTGT CTGGGGGTGGCCATGTTTGCCTACCAGAGCCTCCAGGGCTGTCCCCGCAAAATGGCAGGGGAGATGGTGG AAGGCCTCCGCTACGTCCCCCGTAGCTGTGGCAGTAACTCGTACGTCCTGGTGCCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200999 representing NM_134455
Red=Cloning site Green=Tags(s) MAPSQLAWLLRLAAFFHLCTLLAGQHLGMTKCNITCHKMTSPIPVTLLIHYQLNQESCGKRAIILETRQH RHFCADPKEKWVQDAMKHLDHQTAALTRNGGKFEKRVDNVTPRITSATRGLSPTALAKPESATVEDLTLE PTAISQEARRPMGTSQEPPAAVTGSSPSTSKAQDAGLAAKPQSTGISEVAAVSTTIWPSSAVYQSGSSLW AEEKATESPPTIALSTQASTTSSPKQNVGSEGQPPWVQEQDSTPEKSPGPEETNPVHTDIFQDRGPGSTV HPSVAPTSSEKTPSPELVASGSQAPKVEEPIHATADPQKLSVFITPVPDSQAATRRQAVGLLAFLGLLFC LGVAMFAYQSLQGCPRKMAGEMVEGLRYVPRSCGSNSYVLVPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134455 |
ORF Size | 1179 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134455.1, NP_604450.1 |
RefSeq Size | 3044 bp |
RefSeq ORF | 1182 bp |
Locus ID | 89808 |
UniProt ID | O55145 |
Cytogenetics | 19p13 |
MW | 42 kDa |
Gene Summary | chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200999 | Cx3cl1 (untagged ORF) - Rat chemokine (C-X3-C motif) ligand 1 (Cx3cl1), (10 ug) |
USD 503.00 |
|
RR200999L3 | Lenti ORF clone of Cx3cl1 (Myc-DDK-tagged ORF) - Rat chemokine (C-X3-C motif) ligand 1 (Cx3cl1), (10 ug) |
USD 803.00 |
|
RR200999L4 | Lenti ORF clone of Cx3cl1 (mGFP-tagged ORF) - Rat chemokine (C-X3-C motif) ligand 1 (Cx3cl1), (10 ug) |
USD 803.00 |
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