Kcnh4 (NM_053630) Rat Tagged ORF Clone

CAT#: RR200598

Kcnh4 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily H (eag-related), member 4 (Kcnh4), (10 ug)

Product Images

Circular map for RR200598

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnh4
• Synonyms: Bec2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200598 representing NM_053630
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTCATGAAGGGGTTGCTGGCCCCGCAAAACACCTTTCTGGACACCATCGCCACTCGCTTTGACG
  GCACGCACAGCAACTTTCTTCTGGCCAATGCCCAGGGCCCACGGGGTTTTCCCATCGTCTACTGCTCTGA
  CGGCTTCTGTGAGCTCACAGGCTACGGCCGCACCGAGGTCATGCAGAAAACCTGTAGCTGCCGGTTCCTC
  TATGGCCCAGAGACCAGTGAGCCGGCCTTGCAACGGTTACAAAAAGCCCTGGAGGGCCACCAAGAACACA
  GAGCTGAAATCTGCTTTTACCGAAAGGATGGCTCGGCCTTTTGGTGTCTTCTGGACATGATGCCCATCAA
  AAATGAGCTGGGGGAGGTGGTGCTTTTCCTATTTTCCTTTAAGGACATCTCTCAGAGTGGAGGCCCAGGA
  CTTGGCTCACCAGGGATCCATGGGGACAATAATAATCATGAAAACTCCCTTGGGAGGAGAGGAGCTAGCT
  CAAGACTTAGGTCCACGAGGAGGCAGAACCGGACAGTTCTACACCGGTTGACTGGCCACTTTGGTCGCCG
  GGACCAGGGAAGCGTGAAAGCCAATAGTAACGTGTTTGAGCCAAAGCCATCAGTGCCTGAGTACAAAGTG
  GCCTCCGTGGGGGGCTCCCGCTGCCTGCTCCTCCACTACAGCATCCCCAAGGCTGTCTGGGACGGTCTCA
  TCCTTCTCGCTACGTTCTACGTCGCGGTCACCGTCCCTTACAACGTCTGCTTCGCTGGTGATGACGACAC
  CCCCATCACGTCCCGACACACCCTTGTCAGTGACATCGCTGTGGAGATGCTCTTCATCCTGGACATCATC
  TTGAACTTCCGCACCACCTACGTGTCCCAGTCGGGCCAGGTGGTTTCTGCTCCTCGGTCCATTGGCCTCC
  ACTACCTGGCCACCTGGTTCTTCGTGGACCTCATTGCTGCTTTGCCCTTTGACCTGCTGTATGTCTTCAA
  CATCACTGTGACCTCGCTGGTACATCTGCTGAAAACCGTGCGGCTCCTGCGGTTGCTGAGGCTGCTGCAG
  AAGCTAGAGCGGTACTCTCAGTGCAGCGCGGTGGTGCTCACGCTGCTCATGTCCGTCTTTGCACTCCTTG
  CCCACTGGATGGCCTGCGTCTGGTATGTCATCGGGCGCCGGGAGATGGAGGCCAATGACCCGCTGCTCTG
  GGACATTGGTTGGTTGCATGAGCTGGGTAAGCGGCTGGAGGAGCCTTATGTCAATGGCTCGGCCGGTGGA
  CCATCTCGGCGCAGTGCCTACATCGCCGCGCTGTACTTCACGCTGAGCAGCCTCACCAGTGTAGGCTTCG
  GCAACGTTTGTGCCAACACTGACGCTGAGAAGATCTTCTCCATCTGCACGATGCTCATAGGCGCGCTGAT
  GCACGCGGTGGTGTTTGGGAATGTCACAGCCATCATCCAGCGCATGTACTCCCGACGCTCGCTCTACCAC
  AGCCGCATGAAGGATCTCAAGGACTTCATCCGAGTGCATCGTCTGCCCCGCCCACTCAAGCAGCGCATGC
  TCGAGTACTTCCAGACTACATGGGCCGTCAACAGCGGCATCGATGCCAACGAGTTACTGCGTGACTTCCC
  GGATGAGCTGCGAGCTGACATCGCCATGCACCTGAATCGGGAGATCCTGCAGCTGCCTTTGTTTGGAGCA
  GCAAGCAGGGGCTGCCTTCGTGCCCTCTCCCTGCACATCAAGACCTCATTTTGTGCTCCTGGGGAGTTCC
  TGCTACGCCGTGGGGATGCCCTGCAGGCACACTACTATGTCTGCTCTGGCTCTCTTGAGGTGCTCCGAGA
  CAACACGGTGCTGGCCATCCTTGGAAAGGGGGACTTGATTGGGGCAGACATCCCTGAGTTGGGGCAGGAG
  CCTGGGGCAGGGGCAGGCTGCGTGCTGAAGACCAGCGCTGATGTGAAAGCACTGACTTACTGCGGCCTGC
  AGCAGCTGAGCAGCCGAGGGCTGGCCGAGGTCCTTCGGTTGTATCCGGAATATGTGGCTGCCTTCAGGGC
  TGGCCTACCCCGGGACCTAACCTTCAACCTGCGCCAAGGCTCTGAAAACAATGGCCTCGGCCGCTTCTCA
  CGTTCTCCTCGACTCTCCCAGGCACGCTCCGACACTCTTGGTTCCTCCTCAGACAAGACTCTGCCATCCA
  TCACAGAAACCGAGGGTGGCATGGAGCCTGGGGCTGGTTCCAAGCCCCGTCGGCCCCTCCTGCTGCCCAA
  CCTCAGTCCAGCACGACCTCGGGGGTCCCTGGTCAGCCTTTTGGGCGAAGAGCTGCCCCCATTCTCAGCC
  CTTGTCTCCTCTCCTTCCCTGTCCCCAACTCCTTCCCCTGCCCTGGCTGGCCGGGGTTCAAGTCCCTCCC
  TGCACGGGCCCCCCAGGGGCTCTGCTGCCTGGAAGCCCCCCCAGCTCCTCACCCCCCCACTGGGAACATT
  TGGACCTCCGGACCTCAGTCCCCGGATCGTGGATGGCATTGAGGACTCCAGTAACACAGCTGAGGCTCCT
  ACATTCCGGTTCAGCAAGAGGCCGGAGCCCACCAGAACCCGTTCACAGGCTCCCCTTTCAGGCCCTAGGC
  TCAGCCGGGAACTGGCCACAGAGGCAGCAGAGGAGGTGAAGGAAAAGGTCTGCAGGCTGAACCAAGAGAT
  TTCCAGACTCAACCAGGAAGTGTCTCAGCTGAGCCGGGAGCTTCGCCAAGTGATGGGCCTCTTACAGGCC
  AGGCTGGGTCCCCCAAGTCACCCACCTGACTCCACTTGGCTCCCAGACCTTCCTTGTCCCCATCAGAGAC
  CGCCATGCATCTCTCCTCATATGTCTGGACCTCCACCTGGTCTCCAGAATACTACACTTGCTGTAGTCCA
  CTGTCCAGCCAGTGTTGGGACAGTGGAGATAGGGGCCACCCCCTCAGAGCTGAGGTCTTCCATGGTGCCA
  CCCTTTCCCTCAGAACCTGATCCTCTTGGACCCTCTCCAGTGCCAGAGGCTTCTCCTCTGACCCCAAGCC
  TCCTGAAGCACAGCTTCCAGTCTGGGTCAGACACATTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200598 representing NM_053630
  Red=Cloning site Green=Tags(s)
  
  MPVMKGLLAPQNTFLDTIATRFDGTHSNFLLANAQGPRGFPIVYCSDGFCELTGYGRTEVMQKTCSCRFL
  YGPETSEPALQRLQKALEGHQEHRAEICFYRKDGSAFWCLLDMMPIKNELGEVVLFLFSFKDISQSGGPG
  LGSPGIHGDNNNHENSLGRRGASSRLRSTRRQNRTVLHRLTGHFGRRDQGSVKANSNVFEPKPSVPEYKV
  ASVGGSRCLLLHYSIPKAVWDGLILLATFYVAVTVPYNVCFAGDDDTPITSRHTLVSDIAVEMLFILDII
  LNFRTTYVSQSGQVVSAPRSIGLHYLATWFFVDLIAALPFDLLYVFNITVTSLVHLLKTVRLLRLLRLLQ
  KLERYSQCSAVVLTLLMSVFALLAHWMACVWYVIGRREMEANDPLLWDIGWLHELGKRLEEPYVNGSAGG
  PSRRSAYIAALYFTLSSLTSVGFGNVCANTDAEKIFSICTMLIGALMHAVVFGNVTAIIQRMYSRRSLYH
  SRMKDLKDFIRVHRLPRPLKQRMLEYFQTTWAVNSGIDANELLRDFPDELRADIAMHLNREILQLPLFGA
  ASRGCLRALSLHIKTSFCAPGEFLLRRGDALQAHYYVCSGSLEVLRDNTVLAILGKGDLIGADIPELGQE
  PGAGAGCVLKTSADVKALTYCGLQQLSSRGLAEVLRLYPEYVAAFRAGLPRDLTFNLRQGSENNGLGRFS
  RSPRLSQARSDTLGSSSDKTLPSITETEGGMEPGAGSKPRRPLLLPNLSPARPRGSLVSLLGEELPPFSA
  LVSSPSLSPTPSPALAGRGSSPSLHGPPRGSAAWKPPQLLTPPLGTFGPPDLSPRIVDGIEDSSNTAEAP
  TFRFSKRPEPTRTRSQAPLSGPRLSRELATEAAEEVKEKVCRLNQEISRLNQEVSQLSRELRQVMGLLQA
  RLGPPSHPPDSTWLPDLPCPHQRPPCISPHMSGPPPGLQNTTLAVVHCPASVGTVEIGATPSELRSSMVP
  PFPSEPDPLGPSPVPEASPLTPSLLKHSFQSGSDTFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053630
• ORF Size: 3051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053630.1, NP_446082.1
• RefSeq Size: 3736 bp
• RefSeq ORF: 3054 bp
• Locus ID: 114032
• UniProt ID: Q9R1T9
• Cytogenetics: 10q31
• MW: 111.4 kDa
• Gene Summary: involved in cellular excitability of neurons [RGD, Feb 2006]