Grik1 (NM_017241) Rat Tagged ORF Clone

CAT#: RR200378

Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug)

Product Images

Circular map for RR200378

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grik1
• Synonyms: GluK1; GluR5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200378 representing NM_017241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCAGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT
  TCCTGTGCTACATCCTCCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA
  AAATGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC
  TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC
  GAAGAGCATGCGACCAGCTGGCTCTCGGGGTGGCCGCACTCTTCGGCCCTTCCCACAGCTCCTCCGTCAG
  TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCCGTG
  GACAGCAGAGACCTATTTTATATCAACCTCTACCCGGACTATGCGGCTATCAGCAGGGCGGTCCTGGATT
  TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTGCA
  AGAGCTCATCAAAGCTCCCTCCAGATACAACATTAAAATCAAAATCCGCCAGCTTCCCCCTGCGAATAAA
  GACGCCAAACCTCTGCTCAAGGAGATGAAGAAAAGCAAAGAGTTCTATGTGATATTTGATTGTTCGCACG
  AAACAGCTGCGGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATATTATCACTACTTCTT
  CACAACCCTGGACTTGTTTGCTTTAGATCTGGAACTCTATAGGTACAGCGGTGTAAATATGACTGGATTT
  CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGGTTGCAGG
  CCCCGCCCAGACCCGAGACTGGTCTTCTGGATGGCATGATGACAACTGAAGCAGCGCTGATGTACGATGC
  TGTGTACATGGTAGCCATTGCGTCCCACCGTGCCTCTCAGCTGACCGTCAGCTCCCTGCAGTGCCATCGA
  CATAAGCCATGGCGCCTTGGACCCAGATTTATGAACCTCATCAAAGAGGCTCGGTGGGACGGCTTGACTG
  GGCGGATCACCTTCAATAAGACCGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA
  GGAAGGAACTGAAAAGGCCTCTGGTGAAGTGTCTAAACACTTGTATAAAGTGTGGAAGAAGATTGGGATT
  TGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGACAGGTCCAACAATATCACGGACTCGC
  TGGCTAACCGCACACTCATTGTCACCACTATTCTGGAAGAGCCCTACGTGATGTACAGGAAATCCGATAA
  GCCCTTGTATGGAAACGACAGGTTTGAAGGATATTGCCTGGATCTGCTGAAAGAACTGTCCAATATCCTG
  GGTTTTCTTTACGATGTTAAACTGGTTCCTGATGGCAAATATGGAGCACAGAATGACAAAGGGGAATGGA
  ATGGGATGGTAAAAGAACTCATCGACCACAGAGCTGACCTGGCAGTGGCCCCTCTCACCATCACATACGT
  ACGGGAGAAAGTCATTGACTTCTCCAAGCCCTTCATGACCCTGGGCATTAGCATCCTTTACCGGAAGCCC
  AATGGAACCAACCCGGGTGTCTTCTCCTTCCTCAACCCCCTATCTCCGGACATTTGGATGTACGTGCTGC
  TCGCCTGCCTAGGAGTCAGTTGTGTACTGTTTGTGATTGCGAGGTTCACACCCTACGAGTGGTATAACCC
  CCACCCATGCAACCCCGACTCAGACGTGGTGGAAAACAATTTCACTTTGCTAAATAGTTTCTGGTTTGGA
  GTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAGGCTCTATCGACCAGAATAGTTGGAGGAA
  TATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAACCTGGCTGCCTTCTTGACGGTAGA
  AAGAATGGAATCCCCCATCGATTCCGCAGACGATCTGGCCAAACAAACCAAGATAGAATATGGGGCAGTC
  AGAGATGGCTCGACGATGACCTTCTTCAAGAAATCAAAGATCTCCACCTATGAGAAAATGTGGGCTTTCA
  TGAGCAGTAGACAGCAGAGCGCACTGGTTAAAAACAGTGACGAGGGGATCCAAAGGGTGCTCACCACCGA
  CTACGCACTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGGAACTGCAACCTCACTCAGATC
  GGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACGCCTATCGGCTCCCCTTACCGGGATAAAATTA
  CGATTGCCATTCTTCAACTGCAAGAAGAAGGGAAGCTTCATATGATGAAAGAGAAGTGGTGGAGGGGGAA
  TGGCTGCCCTGAAGAAGACAGTAAGGAAGCCAGTGCTCTGGGAGTGGAAAATATCGGCGGCATCTTCATT
  GTTCTGGCTGCAGGACTCGTGCTTTCTGTGTTTGTAGCCATTGGAGAATTTTTATACAAATCACGGAAGA
  ACAATGACGTTGAGCAGTGTCTCTCTTTCAATGCCATCATGGAAGAGCTGGGAATATCCCTCAAGAATCA
  GAAAAAATTAAAGAAAAAGTCAAGAACTAAGGGCAAATCTTCTTTCACAAGTATCCTTACTTGTCACCAG
  AGACGAACTCAGAGAAAAGAGACAGTGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200378 representing NM_017241
  Red=Cloning site Green=Tags(s)
  
  MERSTVLIQPGLWTRDTSWTLLYFLCYILPQTSPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNRT
  LMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSV
  DSRDLFYINLYPDYAAISRAVLDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPPANK
  DAKPLLKEMKKSKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGF
  RLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGMMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHR
  HKPWRLGPRFMNLIKEARWDGLTGRITFNKTDGLRKDFDLDIISLKEEGTEKASGEVSKHLYKVWKKIGI
  WNSNSGLNMTDGNRDRSNNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNIL
  GFLYDVKLVPDGKYGAQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKP
  NGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFG
  VGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAV
  RDGSTMTFFKKSKISTYEKMWAFMSSRQQSALVKNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQI
  GGLIDSKGYGVGTPIGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFI
  VLAAGLVLSVFVAIGEFLYKSRKNNDVEQCLSFNAIMEELGISLKNQKKLKKKSRTKGKSSFTSILTCHQ
  RRTQRKETVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017241
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017241.2, NP_058937.1
• RefSeq Size: 3244 bp
• RefSeq ORF: 2763 bp
• Locus ID: 29559
• UniProt ID: P22756
• Cytogenetics: 11q11
• MW: 104.5 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]