Pola1 (NM_053479) Rat Untagged Clone
CAT#: RN217636
Pola1 (untagged) - Rat polymerase (DNA directed), alpha 1, catalytic subunit (Pola1)
"NM_053479" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Symbol | Pola1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN217636 representing NM_053479
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTGTTCACGGAGACGACTGCAAACTGGAAACAAGCGCTGTGTCAGATTCAGGGAGTTTTGTAG CTTCTCGAGCACGTCGAGAAAAAAAATCAAAGAAAGGACGCCAAGAAGCTTTAGAGCGACTGAAAAAGGC TAAAGCTGGTGAGAAGTACAAGTATGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG CAGTATTCAAAACTGGTTCAGGCACGTCAGGATGATGACTGGATCGTGGATGACGACGGTATAGGCTATG TGGAAGACGGTAGAGAGATTTTTGATGACGACCTTGAAGATGATGCACTTGACACCTGTGGGGAAGGAAG TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC AAGGCAATGTTCATTGCTAGTGCTGGGAAGAAAACCACAGATAAAACAGTTGACTTGTCCAAGGATGATC TGCTAGGTGACATTCTACAGGATCTGAACACTGAGACACCTCAAATAGCTCCACCGCCAGTATTGATACC AAAGAAAAAAAGATCTACTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCA GGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAAT TTGCTGGCGATCTGGCTCAGCCAGAGTGTCCAGAAGATGAGCAGGAGTCAGGGGTTATAGAGTTTGAAGA TGGCGACTTTGATGAGCCCATGGACACAGAGGAAGTAGATGAGGAGGAGCCTGTGACTGCCAAGATTTGG GACCAAGAGAGTGAGCCAGTGGAAGGGGTGAAGCATGAGGCAGATCCTGAGACGGGAACCACATCTTTCT TAGACAGTTTTCTCCCAGATGTTTCTTGTTGGGACATTGATCAGAAGGACGAAAACAGTTTCTTACTTCA AGAAGTTCAAGTGGACTCCAATCATCTCCCGTTGGTAAAAGGGGCAGATGATGAACAAGTCTTTCAGTTT TATTGGCTGGATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTATGGG TCGAATCAGCCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCT TCCCCGTGAAATGAAAATTGACCTAAACACAGGGAAAGAAACAGCAACTCCAATTACAATGAAGGATGTT TATGAAGAATTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAA AGAACTATGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAGATACTCGGCAGA AGTGCCACAGCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTATTTGGAACTAACACGTCTAGC TTGGAACTGTTCTTGATGAATAGAAAGATCAAAGGACCCTGTTGGCTTGAAGTAAAAAATCCACAACTCT TGAATCAACCAATCAGTTGGTGTAAATTTGAGGCAATGGCTTTGAAACCAGACTTGGTGAATGTAATTAA GGATGTCAGTCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAAAATGTACAGAACCAT CAACACGAGATCATTGCTATGGCAGCGCTGGTTCATCACAATTTCCCATTAGATAAAGCACCTCCAAAAC CTCCCTTTCAGACGCATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGCGCTTTCAAAGA AGTCATTAAGAAAAAGAATATGGAGGTTGAGGTTGCTGCAACAGAAAGAACTTTGCTAGGATTTTTCCTT GCAAAAGTTCACAAACTTGACCCTGATATTCTCGTGGGTCATAATATTTGTGGTTTTGAACTAGAAGTGC TACTGCAAAGAATTAATGAGTGCAAGGTTCCTTTCTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACAT GCCAAAACTTGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATATGTGATGTG GAAATTTCAGTAAAAGAATTGATTCATTGTAAAAGTTACCATTTGTCTGAACTTGTTCAGCAGATTCTGA AAACTGAGAGGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAACCTTCTCATCTGTTGTA CCTGTTGGAACACATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCA TTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGC GAAACGAGTTTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAG AAAACCTCAGCAAAAACCGGGAGATGAAGATGAAGAAATTGATGGGGATACCAATAAATATAAGAAAGGA CGCAAGAAAGCAGCTTATGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTT TGCTCCTGGATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCA AAGAGTTGCTTCAGAGACACTGAAAGCTACAGAGGATGAGGAACAAGAACAGATTCCAGAGTTGCCAGAT CCAAACTTAGATATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGTAGAGCGGAGAAAACAAGTGAAAC AGCTAATGAAACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGATATCCGACAGAAGGCTTTGAA GCTCACAGCGAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCGCTGGCT GCCTTGGTGACATACAAAGGAAGGGAGATTTTGATGCACACGAAAGAGATGGTACAGAAGATGAATCTTG AAGTTATTTATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTCTTTAA GTTGGGAAACAAGGTAAAAAATGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGGTGTT TTCAAGTCTCTGCTATTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTCGAACCAACATCCGATGGGA ATTACATCACCAAGCAGGAACTGAAAGGATTGGATATAGTTAGAAGGGACTGGTGTGATCTTGCCAAGGA CACCGGAAACTTTGTCATTGGCCAGATTCTTTCTGATCAAAGTCGGGACACCATTGTGGAAAACATTCAG AAGAGGTTAATAGAAATTGGAGAAAATGTGCTAAACGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACA AGGCATTGACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGTCTACCTCATGTACATGTTGCACTGTG GATAAATTCTCAAGGAGGCAGGAAGGTGAAAGCTGGCGATACAGTGTCATATGTTATCTGTCAGGATGGG TCAAACCTCCCTGCAACTCAGAGGGCCTATGCACCCGAGCAATTGCAGAAACAGGACAATTTAGCCATCG ACACCCAGTACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGAATCTGTGAGCCAATAGATGGAAT TGATGCTGTACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAA GATGAAGAGAATGATGCTCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTG AAAAATTCAAATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGG AATGGATATGGAGCCCAGCTTGAATCGTTGCAGTAATATTGATTGTAAGGCTTCACCAGCGACCTTTATG GTACAGTTGAGCAACAAATTGATCATGGACATTAGACGTTGCATTAAAAAGTACTATGATGGCTGGTTGA TATGTGAGGAGCCAACTTGTCGCAATCGCATTCGTCGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCT GTGTCCAGCCTGCATGAAAGCTGTGCTCAGACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGC TTTTACCGGTACATTTTTGATGCGGACTGTGCACTGGAAAAACTTCCTGAACACGAGAAAGATAAATTGA AGAAGCAGTTTTTCACTCCCAGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAACACTTCTT GTCCTGGAGTGGCTACTCTGAAGTAAACCTCAGCAAGCTCTTCGCCAATTATGCTGGGAAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_053479 |
Insert Size | 4407 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053479.1, NP_445931.1 |
RefSeq Size | 5321 bp |
RefSeq ORF | 4407 bp |
Locus ID | 85241 |
UniProt ID | O89042 |
Cytogenetics | Xq22 |
Gene Summary | exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR217636 | Pola1 (myc-DDK-tagged) - Rat polymerase (DNA directed), alpha 1, catalytic subunit (Pola1) |
USD 1,381.00 |
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