Tyk2 (NM_001257347) Rat Untagged Clone

CAT#: RN217534

Tyk2 (untagged) - Rat tyrosine kinase 2 (Tyk2)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tyk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217534 representing NM_001257347
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACAAGCATGCCTCTGTGTGGGCGGAGAGCCGCCTCGGAAGACAGCAAGGCAGATGGCACAGAAG
  CTCAGCCCCTGGTGCCCACAGGACGGTTGATGGTGCTGCTGCACTGGCCCGGGCCTGAGGGCGGGGAGCC
  CTGGGTCACCTTCAGCCAGCCATCTCTGACTGCAGAGGAGATGTGCATCCACATCGCACACAAAGTCGGC
  ATCACTCCACCCTGCCTGAATCTCTTCGCACTCTACGATGCACAGGCCAAGGTCTGGCTGCCCCCAAACC
  ACGTTCTGGACACATCCCAAGACGCAACCCTGAGGCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTG
  GCATGGCATGAATCCCCAAGAGCCTGCTGTATACCGATGTGGTTTCCCAGGGGCAGAGACTTCCTCAGAC
  CATGCAGAGCAAGGTGTACAGCTCTTGGACTCTGCCTCATTTGAATACCTTTTTGAGCAGGGAAAACATG
  AATTCATGAACGATGTGGTATCTCTGCGGGACCTGTCTAGCGAGGAAGAGATCCACCACTTTAAGAATGA
  GAGCCTGGGCATGGCCTTTCTGCACCTCTGCCATCTTGCTCTCAACCGTGGCGTCCCCCTGGAGGAGATG
  GCCAGAGAGATCAGCTTCAAGAACTGTATCCCTCACTCCTTCCGTCAGCACATCCGCCAGCACAATGTGC
  TTACGCGCCTGCGTCTCCGCAGAGTCTTCCGCCGCTTCCTGCAGGCCTTCCAGCCTGGCCACCTCTCCCA
  ACAGGTTGTGATGGTTAAGTACTTGGCCACCCTGGAGCGGCTGGCTCCTCGTTTTGGCTCAGAGCACATA
  CCTGTGTGTCATCTGGAAGTTCTGGCCCAGCCTGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGA
  CCACTGGGGACCCAGGCCCAGAGCTGGCTTCTGGGCCAGCCACCCATGAGGTACTGGTGACAGGCACCGG
  AGGTATCCAGTGGCGTCCACTACAGACCCAGGACTCTGAGAGAGGTCACAGCAGAGGGAATCCCCAAGGC
  AGCCAGTCTGGGAAGAAACCCAAGGCCCCTGAGTCTGGAGAGCACCTGGCAGGGAGTCCCCAGGAACCAC
  CTTGGACCTACTTCTGTGACTTCCAGGACATTTCCCATGTGGTACTAAAGGAGCGTCGAGTGCACATCCA
  CCTTCAAGACAACAAGTGCTTGTTGCTGTGCCTCTGCTCCCAGGCCGAGGCCCTGTCCTTTGTGGCCCTG
  GTCGATGGCTATTTCCGCTTGACTGCTGACTCCAGCCACTACCTGTGCCATGAGGTAGCACCCCCGAGGC
  TGGTGACTAGCATCCAGAATGGCATCCACGGGCCCCTGATGGACCCATTTGTACAGGCTAAGCTGTGGCC
  AGAGGATGGCCTCTACCTGATCCAGTGGAGCACCAGCCACCTGCACCGACTGATCCTCACCGTAGCCCAT
  CGAAACCCGGCTTCCAGAAACGGCCCTAGGGGCCTACGCCTCCGAAAGTTCCCCATCACCCAGCAGCCTG
  GAGCCTTTGTGCTTGATGGTTGGGGCCGCTCCTTTGACAGCTTGGGGGACCTTCGGCAGGCCTTGCAGGG
  CTGCTCATTGCGAGCTGGTGATGACTGTTTCCCGTTGCACCACTGCTGCCTGCCCCGACCAAGAGAAATC
  TCCAACCTTGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCACTCAACCTCAGTCAGCTTAGCTTCC
  ACAGGGTTCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGA
  GGGCCTGCTAAGTGTGGGAGGCCCCAATGAGGGCAAAGTGGACAGTGGATGCCCCTCTGGGCCTGATAGG
  GGCAGTGGGCAGCAGCTTCGAGTGGTGCTCAAAGTCCTCGACCCTAGTCACCATGATATCGCCTTGGCCT
  TCTATGAGACTGCCAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTCTGCGT
  GCGTGGCTCAGAGAATATCATTGTAGCGGAATTCGTAGAGCACGGTCCCCTGGATGTGTGGTTACGGAGA
  CAGAGAGGCCGAGTGCCCATGCCCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTGAGCTACC
  TGGAGGACAAGAATCTGGTTCACGGGAACGTATGTGGCCGGAACATCCTGCTGGCCCGCCTGGGGCTGGA
  GGAGGGTACCAACCCCTTCATCAAGCTCAGTGACCCTGGCGTGGGCCAAGGCGCCCTCTCCAGGGAAGAG
  CGGGTGGAGCGAATCCCCTGGACAGCTCCGGAGTGCCTGTCCGGAGGGGCCAACAGCTTGGGCACTGCCA
  CGGACATGTGGGGCTTTGGTGCCACCCTCCTTGAGATCTGTTTTGATGGAGAGGCACCTCTGCAGGGCCG
  TGGTCCCTCCGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCAGAGCCCTCATGCCCGGAGCTG
  GCCACACTCACCCGTCAGTGCCTGAGCTATGAACCCGCACAGAGGCCATCGTTCCGCACCATTTTGCGGG
  ACCTCACAAGACTGCAGCCACAGAATCTAGTGGGAACTTCGACTGTGAACTCGGACTCACCAGCATCGGA
  CCCCACCGTTTTTCACAAGCGTTACTTGAAAAGGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTC
  AGCCTGTACTGCTATGACCCGAACAATGACGGCACTGGCGAGATGGTGGCTGTGAAAGCCCTGAAGGAGG
  GATGTGGTCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAAATCCTTCGGACGCTGTACCACGAGCA
  CATTGTCAAGTACAAAGGCTGCTGTGAAGACCAAGGGGAGAAGTCTGTACAGCTGGTCATGGAATACGTC
  CCTCTGGGCAGCCTCCGAGACTACCTGCCAAGGCACAGCGTGGGGCTGGCTCAGCTCCTGTTGTTTGCCC
  AGCAGATCTGCGAGGGCATGGCTTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCTGCGCGAAA
  CGTGCTGCTGGACAACGACCGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGC
  CACGAGTACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTATTCTGGTATGCCCCAGAATGCCTAAAGG
  AGTGCAAGTTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTGACCCTTTATGAGCTGTTGACATACTG
  TGACTCTAGCCAGAGTCCCCCCACGAAATTCATCGAGCTCATCGGCGTAACCCAGGGCCAGATGACTGTG
  CTGAGGCTCACAGAGCTGCTGGAGCGAGGGGAGAGGCTGCCGCGGCCTGACAGATGTCCCTGTGAGATCT
  ATCACCTCATGAAGAACTGCTGGGAGTCAGAGGCCTCCTTCCGGCCCACGTTCCAGAATCTTGTGCCCAT
  CCTTAAGACAGCACAGGAGAAGTACCAAGGCCAGGTGCCTTCAGTGTTCAGCGTGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257347
• Insert Size: 3561 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257347.1, NP_001244276.1
• RefSeq Size: 4535 bp
• RefSeq ORF: 3561 bp
• Locus ID: 100361294
• Cytogenetics: 8q13