Itgb7 (NM_013171) Rat Untagged Clone

CAT#: RN217221

Itgb7 (untagged) - Rat integrin, beta 7 (Itgb7)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Itgb7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217221 representing NM_013171
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATTCATCAACTGTTCTCATTTTTCTGCTCGTTCTGGGCGGAGGTCAGAGTGAGTTAGATACCA
  AGATTACGTCCTCAGGGGAGACCACAGAATGGGAGGATCCTGACCTGTCTCCACAGGGATCCTGCCAGCC
  AGCTCCTTCCTGCCAGAAGTGTATACTCTCACACCCCAGCTGTGCATGGTGCAAGCAACTGAACTTCACT
  GCCTCGGGGGAGGCAGAGGCGAGGCGCTGCGCGCGGCGTGAGGAGCTACTGGCCCGCGGGTGCCCCGAGC
  AGGAGCTGGAGGAGCCGAGAGGCCACCAGGAGGTGCTGCAGGACAGGCCACTCAGCCAGGGAGACCGCGG
  CGAGGGGGCCACCCAGCTGGCCCCGCAGCGGATCCGCGTCACGTTGCGACCAGGGGAGCCCCAGAAATTC
  CGGGTCCGCTTCCTCCGAGCTGCGGGATATCCCGTGGACCTGTACTACCTTATGGACCTGAGCTATTCAA
  TGAAGGACGACTTGGAACGTGTGCGACAGCTCGGGCAAGCCCTGCTGCTCCGGCTGCGGGAGGTCACCCA
  TTCTGTGCGCATAGGTTTTGGCTCCTTTGTGGACAAAACGGTGCTACCCTTTGTAAGCACAGTGCCCTCC
  AAGCTTAACCACCCATGCCCCAGCCGGTTGGAGCGCTGTCAGGCACCCTTCAGCTATCACCACGTGTTGC
  CCCTCACCAGGGATGCTCAAGCCTTCGAGAGAGAGGTGGGACGCCAGAACGTCTCTGGCAACCTGGACTC
  ACCCGAAGGAGGCTTTGATGCCATTTTGCAGGCTGCTCTTTGCCAGGAGCAGATTGGCTGGAGAAATGTG
  TCCCGGCTTCTGGTGTTCACTTCAGACGATACGTTCCACACAGCTGGGGATGGGAAACTGGGTGGCATTT
  TCATGCCCAGCGATGGGCGGTGCCACCTGGACAGCAACGGTGTCTACACTAACAGCGCAGAGTTTGACTA
  TCCCTCTGTGGGCCAAGTAGCCCAGGCTCTCACAGCAGCGAATATCCAACCTATCTTTGCTGTCACCGGC
  GCAACACTGCCCGTGTATCAGGAGCTGAGCCAGTTGATTCCCAAGTCTGCTGTCGGGGAGCTCAGCGAAG
  ACTCCAGCAATGTGGTGCAGCTCATCATGGATGCTTACGACAACCTGTCATCCACCGTGACTCTTGAACA
  CTCTTCGCTCCCACCTGGAGTCAGCATCTCTTTCGAGTCTCACTGTAGGAGTCCTGAGAAGAGTGAGGGT
  GAGGCTGGGGACCGTCGGGGACAGTGCAATCATGTCCGAGTCAACCAGATGGTGGATTTCTGGGTCACTC
  TTCAAGCCTCTCACTGCCTCCCAGAAGCCCATGTCCTACGGCTCTGGGCCCTTGGCTTCTCAGAGGAGTT
  AACTGTGGAGCTGCACACACTGTGTGACTGCAATTGTAGTGATGCCCAGCCTCGCGCTCCTTACTGCAGC
  GATGGACAGGGGGACCTTCAATGCGGGATATGCAGCTGTGCCCCTGGCCGCCTTGGTCAGCTGTGTGAAT
  GCTCTGAGGCTGACCTGTCCTCCCCAGATTTGGAATCTGGTTGCCGGGCCCCCAATGGGACAGGGCCCCT
  GTGCAGCGGGAAGGGTCGATGCCAATGTGGACGCTGCAGTTGCAGTGGACAGAGCTCTGGGCGTCTGTGC
  GAGTGTGATGATGCCAGCTGTGAGCGACACGAGGGCATCCTCTGTGGAGGCTTTGGCCACTGCCAGTGTG
  GAGTGTGTCACTGCCATGCCAACCGCACTGGCAGAGCATGTGAGTGCAGTGAGAGTGTGGACAGCTGCGT
  TAGTCCCGAAGGAGGGCTCTGCAGTGGGCATGGTGACTGCAAATGCAACCGTTGCCAGTGCCTGGATGGC
  TACTACGGGGCTCTGTGTGACCAGTGCCTAGGCTGCAAGTCACCATGTGAGCAATACAGGGACTGCGCGG
  AGTGTGGGGCGTTTGGTACGGGTCCCCTGGCAGCCAACTGCAGCGCGGCTTGTGCTGACGTGAATGTGAC
  CTTGGCCTTGGCCCCCAATTTGGATGATGGTTGGTGCAAAGAGAGGACGCTAGACAACCAGCTGTTCTTC
  TTCCTGGTGGAGAATTCGGCCAGAGGGGTCATACTGAGAGTGAGACCCCAAGAGAAGGGAGCAGACCACA
  CCCGTGCCATCATACTGGGCTGCGTAGGGGGCATTGTGGCAGTGGGATTAGGGCTGGTCCTGGCTTACCG
  GCTCTCAGTGGAAGTCTATGACCGCCTTGAGTACAGCCGCTTTGAGAAGGAGAGGCAGCAACTCAACTGG
  AAGCAGGACAGCAATCCTCTCTACAAAAGTGCGGTCACAACTACCGTCAACCCTCGCTTCCAAGGGGGCA
  ACAAACAGTCGCTGTCCCTCCCTCTGACCCAGGAGGCTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013171
• Insert Size: 2424 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013171.1, NP_037303.1
• RefSeq Size: 2654 bp
• RefSeq ORF: 2424 bp
• Locus ID: 25713
• Cytogenetics: 7q36
• Gene Summary: mediates adhesion to mucosal addressin cell adhesion molecule-1 (MAdCAM-1) [RGD, Feb 2006]