Casq1 (NM_001159594) Rat Untagged Clone
CAT#: RN216585
Casq1 (untagged) - Rat calsequestrin 1 (fast-twitch, skeletal muscle) (Casq1)
"NM_001159594" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Symbol | Casq1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN216585 representing NM_001159594
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCCACTGACAGGATGGGGGCCAGGGCAGTGTCCAAGCTGCGGCTGGCACTGCTGTTTGTACTGG TGCTAGGGACGCCCAGGTCAGGGGTCCAGGGGGAGGACGGGCTGGACTTCCCTGAATACGACGGTGTGGA CCGCGTGATCAATGTGAACGCAAAGAACTACAAGAACGTGTTTAAGAAGTATGAGGTGCTAGCCCTCCTC TACCATGAGCCCCCCGAGGACGACAAGGCCTCCCAAAGACAATTTGAGATGGAGGAGCTAATTCTGGAGT TAGCAGCCCAAGTCCTAGAAGACAAGGGTGTTGGCTTTGGCCTGGTGGACTCTGAGAAAGATGCAGCTGT GGCCAAGAAACTAGGCCTAACTGAAGAGGACAGCGTGTATGTGTTCAAAGGAGATGAAGTCATCGAGTAT GATGGCGAGTTTTCCGCAGACACTTTGGTGGAGTTTCTGCTCGATGTCCTAGAAGACCCTGTCGAATTGA TTGAAGGTGAACGAGAGCTGCAGGCATTTGAGAACATCGAGGATGAAATCAAACTCATTGGCTACTTCAA GAGCAAAGACTCAGAGCATTACAAAGCCTACGAGGATGCAGCTGAAGAATTCCACCCCTACATCCCTTTC TTCGCTACCTTCGACAGCAAGGTGGCAAAGAAGCTGACGCTGAAGTTGAACGAGATCGATTTCTATGAGG CCTTCATGGAAGAACCTGTGACCATCCCAGACAAGCCCAACAGTGAAGAGGAGATTGTCAGCTTCGTGGA GGAGCACAGGAGATCGACCCTGAGGAAACTGAAGCCTGAGAGTATGTATGAGACTTGGGAGGATGATCTG GATGGAATCCACATTGTGGCCTTTGCAGAGGAAGCTGACCCTGATGGTTATGAGTTCTTAGAGACTCTCA AGGCTGTGGCCCAAGACAACACTGAGAACCCTGACCTCAGTATCATCTGGATTGACCCTGATGACTTCCC ACTGCTGGTCCCGTACTGGGAGAAGACCTTTGACATCGACTTGTCAGCTCCGCAAATAGGAGTTGTCAAT GTTACGGATGCGGACAGCATATGGATGGAGATGGACGACGAGGAGGACCTTCCTTCTGCTGAGGAGCTGG AGGACTGGCTGGAGGATGTGCTGGAGGGTGAAATCAACACAGAGGATGACGACGACGACGACGACGACGA CGATGATGACGACGACGACGACGATGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001159594 |
Insert Size | 1221 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159594.1, NP_001153066.1 |
RefSeq Size | 1865 bp |
RefSeq ORF | 1221 bp |
Locus ID | 686019 |
UniProt ID | P19633 |
Cytogenetics | 13q24 |
Gene Summary | Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:8042990). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction (By similarity). Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR216585 | Casq1 (myc-DDK-tagged) - Rat calsequestrin 1 (fast-twitch, skeletal muscle) (Casq1) |
USD 503.00 |
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