Casq1 (NM_001159594) Rat Untagged Clone

CAT#: RN216585

Casq1 (untagged) - Rat calsequestrin 1 (fast-twitch, skeletal muscle) (Casq1)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Casq1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216585 representing NM_001159594
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCCACTGACAGGATGGGGGCCAGGGCAGTGTCCAAGCTGCGGCTGGCACTGCTGTTTGTACTGG
  TGCTAGGGACGCCCAGGTCAGGGGTCCAGGGGGAGGACGGGCTGGACTTCCCTGAATACGACGGTGTGGA
  CCGCGTGATCAATGTGAACGCAAAGAACTACAAGAACGTGTTTAAGAAGTATGAGGTGCTAGCCCTCCTC
  TACCATGAGCCCCCCGAGGACGACAAGGCCTCCCAAAGACAATTTGAGATGGAGGAGCTAATTCTGGAGT
  TAGCAGCCCAAGTCCTAGAAGACAAGGGTGTTGGCTTTGGCCTGGTGGACTCTGAGAAAGATGCAGCTGT
  GGCCAAGAAACTAGGCCTAACTGAAGAGGACAGCGTGTATGTGTTCAAAGGAGATGAAGTCATCGAGTAT
  GATGGCGAGTTTTCCGCAGACACTTTGGTGGAGTTTCTGCTCGATGTCCTAGAAGACCCTGTCGAATTGA
  TTGAAGGTGAACGAGAGCTGCAGGCATTTGAGAACATCGAGGATGAAATCAAACTCATTGGCTACTTCAA
  GAGCAAAGACTCAGAGCATTACAAAGCCTACGAGGATGCAGCTGAAGAATTCCACCCCTACATCCCTTTC
  TTCGCTACCTTCGACAGCAAGGTGGCAAAGAAGCTGACGCTGAAGTTGAACGAGATCGATTTCTATGAGG
  CCTTCATGGAAGAACCTGTGACCATCCCAGACAAGCCCAACAGTGAAGAGGAGATTGTCAGCTTCGTGGA
  GGAGCACAGGAGATCGACCCTGAGGAAACTGAAGCCTGAGAGTATGTATGAGACTTGGGAGGATGATCTG
  GATGGAATCCACATTGTGGCCTTTGCAGAGGAAGCTGACCCTGATGGTTATGAGTTCTTAGAGACTCTCA
  AGGCTGTGGCCCAAGACAACACTGAGAACCCTGACCTCAGTATCATCTGGATTGACCCTGATGACTTCCC
  ACTGCTGGTCCCGTACTGGGAGAAGACCTTTGACATCGACTTGTCAGCTCCGCAAATAGGAGTTGTCAAT
  GTTACGGATGCGGACAGCATATGGATGGAGATGGACGACGAGGAGGACCTTCCTTCTGCTGAGGAGCTGG
  AGGACTGGCTGGAGGATGTGCTGGAGGGTGAAATCAACACAGAGGATGACGACGACGACGACGACGACGA
  CGATGATGACGACGACGACGACGATGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159594
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159594.1, NP_001153066.1
• RefSeq Size: 1865 bp
• RefSeq ORF: 1221 bp
• Locus ID: 686019
• UniProt ID: P19633
• Cytogenetics: 13q24
• Gene Summary: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:8042990). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction (By similarity). Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity).[UniProtKB/Swiss-Prot Function]