Col5a3 (NM_021760) Rat Untagged Clone

CAT#: RN214167

Col5a3 (untagged ORF) - Rat collagen, type V, alpha 3 (Col5a3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Col5a3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214167 representing NM_021760
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGCTGCCGGAGACTGGACCAGCTTCAGGCCGGCCTCTGCCTGCTCCTGGCCTCCCTACAACTCG
  TGTCCTGGACGCTGGCTGCAGAACCTGTGGATGTATTGGAAACCTTGGGTGTGCATAGGGACAAGGCTGG
  GGTGACCAAAGGGCCTGGCTTCTGCCCCCAGAGGATTCCACAGGGTGACCGAGCATTCAGGGTGGGCAAG
  TCCAGCCTTCTCAGCGTCCCCACATGGCAGCTCTTTCCAGATGGACATTTTCCTGAGAACTTTTCTGTGC
  TGCTCACTCTGCGGGCCCAGCCAGCCAATCAATCTGTCCTTCTGTCTATATATGACGAGAATGGTGTCCG
  GCAGATGGGGCTGGCACTGGGGCCAGCTCTGGGTCTCCTTGGTGACTCTTTCAGGTCTCTTCCCAAGCAG
  ATCAACCTTATGGATGGCAGGTGGCACCGGGTGGCGGTCAGCATCAGCGGTGACAAGGTGACCCTGGTGG
  TTGACTGTGAACCTCAGCCCCCCATGTTTGGTCAGGGGCCTCGGGTTATAAGTACAGCTGGACTCACGGT
  GATGGGAACCCAGGAGCTCGGGGAGGAGTCTTTTGAGGGAGACATCCAGGAGCTGCTGTTAATTCCAGAC
  CCTCAGGCTGCTCTCCAGGCCTGTGAGCTCTACCTGCCTGACTGTGAGACCCCCGATTCCACAACCACAG
  GGGTCACTAAAGAAGAACCAGAAACACCTGCCCCTCGTCGACGAAAGGGCAAAGGAAAGAAAAAAGATCG
  GGGTCGCAAGGGCAAGGGAAGAAAGAAAAACAAGACCTCAAGACTGAGTCCAACCCCAGGTGCCCCTGAG
  AACCAGACCTCCCTCCACACCCCTGAGACAGAGAAGCCATCGCCCCATCTGCCTCCAACTCCCACACCTT
  TGGCCATCACCACCACTGTCACGATGGGAGGAAATGCCACAGTTTTGCAGGGGTTGGACTCCAGTACTGA
  AACTGAGCAGATGACTCCAGAGATGGACGCTACTGAAGAGAGTGAAGGAGGTGGCCCGACCATGGGCCCC
  GAGTTCCGGGCAGCAGAACAGTCTTTACAGACTGAGTTCCAGATCTTTCCTGGTGCTGGAGAAAAGGGAG
  CGAAAGGAGAACCTGCGACTATAGAGCAGGGACAGCAGTTTGAGGGACCTGCAGGAGCTCCAGGACCCCG
  GGGAATATCTGGTCCTTCAGGCCCTCCTGGGCCTCCGGGCTTCCCTGGGGACCGTGGCCTACCGGGCCCT
  GCTGGCCTCCCAGGAATCCCAGGCATTGATGGCGTCCGGGGCCTGCCAGGCACAGTGATCATGATGCCGT
  TCCATTTTGCAAGCGGCTCGATGAAAGGACCCCCAGTCTCCTTTCAACAGGCACAGGCCCAGGCAGTACT
  GCAACAGGCTCAGCTGTCCATGAAAGGGCCCCCTGGTCCAGTAGGGCTCACTGGGCGCCCAGGCCCTGTG
  GGCCTCCCTGGATATCCAGGTCTGAAAGGTGAATTGGGAGAAGCAGGGCCTCAGGGCCCCCGAGGATTGC
  AGGGCCCTCCTGGGCCTCCTGGACGGGAAGGCAAGACAGGCCGAGCTGGAGCAGATGGGGCTCGGGGTCT
  TCCGGGAGACACAGGACCTAAGGGTGACCGGGGCTTTGATGGCCTGCCTGGACTGCCTGGTGAGAAGGGC
  CAGAGGGGTGACTTTGGACCTGTAGGACAACCTGGTCCCCCAGGAGAAGATGGTATAAAGGGCCTGCAGG
  GACCTCCAGGCCCCACTGGCCAGGCTGGAGAGCCGGGTCCCCGAGGTCTGATTGGCCCCAGAGGCCCCCC
  TGGTCCTTTAGGACGCCCGGGTGTGACAGGGAGTGATGGCGCACCGGGTGCCAAAGGCAACGTGGGTCCT
  CCCGGAGAACCAGGCCCCCCAGGACAGCAAGGAAACCACGGCTCCCAGGGAATCCCAGGCCCCCAGGGGC
  CCATTGGCACACCCGGGGAGAAGGGTCCTCCTGGAAACCCCGGAATTCCAGGTGTCCCAGGATCTGAGGG
  CCCCCCGGGCCACCCAGGCCATGAGGGTCCCACCGGAGAAAAGGGGGCTCAGGGCCCACCAGGGTCAGCA
  GGCCCTCAGGGCTATCCTGGACCTCGTGGTGTGAAGGGTACCTCTGGTAACCGGGGCCTCCAAGGAGAGA
  AAGGAGAAAGGGGAGAGGATGGTTTTCCAGGCTTCAAGGGTGATGAGGGACCAAAAGGCGACCGGGGAAA
  CCCGGGACTCCCAGGTCCCAGAGGAGAGGATGGCCCAGAAGGACAAAAGGGGCCTGAGGGACTGCCTGGT
  GATGAGGGCCCCCCAGGAGCAGCAGGGGAGAAGGGCAAGCTTGGGGTGCCAGGTCTCCCAGGTTATCCAG
  GACGCCCAGGACCTAAGGGATCTATTGGATTTCCTGGGCCCCTTGGACCACTGGGGGAGAAAGGCAAGCG
  GGGCAAAGCAGGGCAGCCAGGGGAGGAAGGAGAACGTGGCACACCGGGCACCAGAGGAGACAGAGGACAG
  CCAGGGGCCACAGGCCAGCCTGGCCCTAAGGGAGATGTGGGCCAGAACGGGTCTCCTGGGGCCCCCGGAG
  AAAAGGGTCTACCCGGTCTGCAAGGTCCCCCAGGATTCCCTGGGCCAAAAGGCCCCCCAGGTCCTCAGGG
  GAAAGATGGGATACCCGGACACCCTGGACAAAGAGGAGAATTGGGCTTCCAAGGTCAGACAGGCCCCCCT
  GGACCAGCTGGTGTCCTTGGTCCTCAGGGAAAGGTAGGGGATGTGGGTCCTCTAGGCGAGAGAGGCCCCC
  CAGGGCCTCCTGGACCGCCTGGTGAACCAGGTCTGCCAGGCATAGAAGGCAGAGAAGGGGCCAAGGGTGA
  GCTAGGACCCCTGGGGTCCCTCGGAAAGGAGGGGCCACCTGGGCCCATGGGCTTCCCTGGCCCACAAGGA
  GCCCCCGGAGACCCAGGACCCATTGGTTTGAAGGGTGACAAGGGTCCCCCGGGCCCTCTTGGGGCAAATG
  GCTCCCCAGGTGAGCGTGGTCCTGTGGGCCCCTCTGGAGGCATTGGGCTTCCTGGCCAGAGTGGTGGGCA
  AGGCCCTATTGGTCCTGCTGGTGAGAAGGGGTCCCCGGGAGAACGGGGTGTGCCTGGTCCTACTGGCAAA
  GATGGTATTCCAGGGCCCCCGGGACTTCAGGGGCCCCCTGGAGCTGCAGGGCCTTCTGGTGAGGAAGGAG
  ACAAGGGGGAAGTAGGGATGCCTGGACACAAGGGAAGCAAAGGGGATAAAGGAGATGCGGGCCCACCTGG
  ACCAACAGGAATAAGGGGTCCAGCGGGCCATCCAGGCCCCCCGGGTGCTGATGGTGCTCAGGGTCGCCGG
  GGACCCCCTGGCCTCTTTGGGCAGAAAGGTGATGATGGAGTCCGAGGCTTTGTAGGTGTAATTGGTCCTC
  CAGGCCTGCAGGGGCTGCCGGGCCCTCCTGGGGAGAAAGGCGAGGTTGGAGACGTGGGATCCATGGGTCC
  CCACGGAGCTCCAGGCCCTCGGGGTCCCCCTGGGCCCAGTGGATCAGCGGGTTCCCCAGGGCTGCCTGGA
  GGAGTAGGGCAGCCTGGCGCTGTGGGCGAGAAGGGTGAGCCAGGGAATGCTGGAGACGCTGGACCCCCAG
  GAGTTCCAGGCATCCCTGGGCCCAAAGGTGAAATTGGTGAAAAGGGGGATTCGGGTCCATCAGGGGCTGC
  TGGTCCTCCAGGCAAAAAGGGACCTCCTGGAGAGGACGGAGCTAAGGGGAACATGGGTCCCACAGGACTC
  CCTGGAGATCTAGGACCCCCAGGAGACCCTGGAGTTCCGGGTACGGATGGCATCCCAGGGGAGAAGGGGA
  ATGCTGGTGATATTGGGGGACCGGGGCCACCTGGAGCTTCAGGGGAACCTGGTGCCCGTGGGCTCCCTGG
  CAAGAGGGGTTCCCCTGGCCGCATGGGTCCAGAAGGAAAAGAGGGAGAGAAAGGCGCCAAGGGAGATGCT
  GGTCCCGATGGCCCCCCAGGCAGAACAGGCCCCATTGGGGCTCGGGGGCCCCCTGGACGGGTTGGGCCTG
  ATGGTCTTCCAGGGATCCCTGGTCCTGTGGGTGAACCAGGTCTCCTGGGACCTCCTGGGCTAATCGGCCC
  TCCAGGGCCCCTGGGCCCACCTGGCCTCCCTGGCCTGAAGGGAGACGCTGGCCTCAAGGGGGAAAAGGGC
  CACATTGGGCTAATCGGCCTCATTGGTCCCCCAGGGGAGGCTGGGGAAAAAGGCGATCAGGGGTTGCCAG
  GTGTGCAGGGCCCTCCAGGCCTTCAGGGAGACCCTGGTCTTCCTGGTCCTGTTGGCTCCTTAGGTCACCC
  TGGGCCCCCAGGTGTGGTGGGCCCTCTGGGACAGAAGGGTTCCAAGGGGTCCCCGGGATCTCTTGGACCT
  CGTGGAGACCCTGGACCAGCAGGTCCTCCTGGTCCCCCGGGTTCCCCTGCTGAGGTGCACGGCCTGCGTA
  GGCGCCGCTCTGTGTTGGACACCCCAGAAGGTGGCCTGGAGGAAGTGATGGCCTCACTCAATTCACTGAG
  CTTGGAGCTGGAGCAGTTGCAGCGACCCCCAGGCACAGCCGAGAGCCCAGGCCTCATATGCCACGAGCTT
  CATCGAAACCACCCACACCTGCCGGACGGAGAGTACTGGATTGACCCCAATCAGGGCTGTGCACGGGACG
  CCTTCAAGGTCTTCTGCAACTTCACTGCCGGAGGTGAGACCTGTCTCTACCCAGACAAGAAGTTTGAGAC
  GGTGAAACTGGCCTCCTGGTCCCGAGAAAAGCCTGGAGGTTGGTACAGCACCTTCCGCCGAGGGAAGAAG
  TTCTCCTATGTGGATGCTGACGGCTCCCCGGTGAATGTGGTCCAGTTGACCTTCCTGAAGTTGTTGAGCG
  CTGCAGCCCATCAGAGGTTCACTTACACCTGCCAGAACTCCGCAGCATGGCTGGACGAAGCTTCAGGTGA
  CCACAGGCACTCTATCCGCTTCCAAGGGACCAACTGGGAAGAGTTGTCCTTCAACCAGACAACAGCAGCC
  ACCATCAAGGTCCCCCATGATGGCTGCCGGGTCCGGAAGGGACAGGCGAAGACCCTCTTTGAATTCAGCT
  CTTCTGTGGGCTTTCTGCCTCTGTGGGATGTGGCCGCCGTTGACTTTGGCCAGACGAACCAGAAGTTTGG
  GTTTGAACTTGGCTCCGTCTGCTTTAGCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021760
• Insert Size: 5214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021760.1, NP_068528.1
• RefSeq Size: 6076 bp
• RefSeq ORF: 5214 bp
• Locus ID: 60379
• Cytogenetics: 8q13
• Gene Summary: alpha chain of type V collagen [RGD, Feb 2006]