Trim72 (NM_001077675) Rat Untagged Clone

CAT#: RN210882

Trim72 (untagged ORF) - Rat tripartite motif-containing 72 (Trim72), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Trim72
• Synonyms: RGD1562778
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210882 representing NM_001077675
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACTGCACCAGGCCTTTTGCGCCAGGAACTGTCTTGCCCGCTGTGCTTGCAGCTGTTCGATGCAC
  CAGTGACCGCTGAGTGTGGCCACAGTTTCTGCCGTGCCTGCCTGATCCGTGTGGCAGGGGAGCCTGCCGA
  CGATGGCACGGTTGCCTGTCCCTGTTGTCAGGCATCTACTCGGCCACAGGCGCTAAGCACTAACCTCCAG
  TTGGCACGCCTTGTGGAGGGTTTGGCACAAGTGCCCCAAGGCCACTGCGAGGAACACCTGGATCCACTGA
  GCATCTACTGCGAGCAGGACCGCACACTTGTGTGTGGTGTGTGTGCCTCTCTCGGTTCACACCGTGGTCA
  CCGTCTTCTGCCTGCCGCAGAAGCCCATGCACGTCTCAAGACACAGCTTCCACAACAGAAGGCCCAGCTG
  CAGGAGGCATGCATGCGCAAGGAGAAGAGTGTAGCAGTCCTGGAGCATCAGCTGGTGGAGGTGGAGGAGA
  CCGTGCGTCAGTTCCGGGGAGCTGTTGGGGAGCAGCTGGGGAAGATGCGGATGTTCCTGGCTGCCCTAGA
  AAGTTCTTTGGACCGTGAAGCAGAAAGGGTTCGAGGTGAGGCAGGGGTTGCCTTGCGGCGGGAGCTGTCA
  AGCCTGAACTCTTACCTGGAGCAACTGAGGCAGATGGAGAAGGTGCTGGAGGAGGTGGCTGACAAGCCAC
  AGACAGAATTCCTCATGAAATTCTGCCTGGTGACCAGCAGGCTGCAGAAGATTCTGTCAGAGTCACCACC
  CCCAGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAGAAGATG
  TTCCGGGCTCTGATGCCAGAGTTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCCTGGTGG
  TGTCCGCCTCTGGTCGCCGAGTGGAGTGCTCGGAGCAGAAGGCGCCGCCAGCAGGAGAAGACACGTGCCA
  GTTCGACAAGACGGTAGCGGTAGTGGCGAAGCAGCTGCTGTCACAGGGGGAGCACTACTGGGAGGTGGAG
  GTGGGCGACAAGCCACGCTGGGCCCTGGGTGTGATGGCGGCTGACGCTTCCCGTCGTGGCCGCCTGCACG
  CGGTGCCCTCACAGGGGCTGTGGTTGCTCGGCCTGCGCGATGGCAAGATCCTGGAGGCACACGTGGAGGC
  CAAGGAGCCACGGGCACTGCGCACCCCAGAGAGGCCACCAGCGCGCATTGGCCTCTACCTAAGCTTTGCA
  GATGGCGTCCTGACTTTCTATGATGCAAGCAACACCGACGCGCTTACACCGCTCTTTTCTTTTCATGAGC
  GTCTGCCTGGGCCGGTGTACCCCATGTTTGACGTGTGCTGGCACGACAAGGGCAAGAATTCTCAGCCGCT
  GTTGCTCGTGGGGCCAGACAGTGAGCAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077675
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077675.1, NP_001071143.1
• RefSeq Size: 2363 bp
• RefSeq ORF: 1434 bp
• Locus ID: 365377
• UniProt ID: A0JPQ4
• Cytogenetics: 1q37
• Gene Summary: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).[UniProtKB/Swiss-Prot Function]