Pgap1 (NM_201990) Rat Untagged Clone

CAT#: RN209425

Pgap1 (untagged ORF) - Rat post-GPI attachment to proteins 1 (Pgap1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pgap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209425 representing NM_201990
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTCCACTCAGTCAACCTCTGGAACCTGGCGTTCTATGTCTTCATGGTGTTTCTGGCGACCCTGG
  GGCTGTGGGATGTCTTCTTCGGCTTCGAGGAGAACAAGTGCAGCATGAGCTACATGTTCGAGTACCCCGA
  GTACCAGAAAATAGAACTTCCAAAGAAACTGACAAAGCGTTATCCAGCCTATGAGTTATATCTTTATGGA
  GAAGGATCATACGCTGAAGAACACAAAATTCTCCCTTTGACAGGAATTCCTGTTCTCTTTCTTCCTGGGA
  ATGCTGGAAGCTATAAGCAAGTTCGTTCTATCGGCTCTATTGCGCTTAGGAAAGCAGAGGACATCGATTT
  CAAGTACCACTTCGACTTCTTCAGTGTCAACTTCAATGAAGAACTGGTGGCATTATATGGTGGAAGCCTT
  CAGAAGCAGACCAAGTTTGTACACGAATGCATTAAAGCAATTCTCAAACTCTACAAGGGTCAAGAATTTC
  CTCCCACAAGTGTGGCAATAATTGGTCACTCTATGGGTGGTCTTGTTGCCAGAGCGTTGCTTACACTGAA
  AAATTTTAAACAAGATCTGATCAATCTTCTCGTTACACAAGCTACTCCTCATGTGGCCCCTGTGATGCCG
  TTAGATCGTTTCATTACGGAGTTTTATATGACTGTAAACAACTATTGGATTTTAAATGCTCGGCACATAA
  ATTTGACCACACTTTCTGTAGCTGGAGGCTTCCGGGATTACCAAGTTCGTTCGGGACTGACTTTTTTACC
  GACATTAAGCCATCATACCAGTGCCTTATCTGTTGTGACCTCAGCAGTGCCTAAGACATGGGTCTCAACA
  GATCACCTCTCCATCGTGTGGTGTAAACAGCTGCAGCTGACGACAATTCGAGCGTTCTTTGATCTTATTG
  ATGCCGATACCAAACAAATAACTCAGAAGTCTAAGAAGAAATTGTCTGTTCTGAATCACCATTTTATAAG
  ACACCCAGCTAAACAGTTTGAGGAAAATCCGTCCATAATTTCTGACCTAACAGGGACATCTATGTGGGTT
  CCAGTAAAAGTGTCCAGATGGTCCTATGTGGCTTATAATGAATCTGATAAGATTTACTTTGCATTTCCTC
  TTGCAAATCATAGAAAGGTCTACACTCACGCCTACTGTCAGAGCACCATGCTGGACACAAATAGCTGGAT
  TTTTGGCTGCATAAACAGCACTTCCATGTGCCGGCAAGGGGTTGATTTGTCCTGGAAAGCTGAACTGTTG
  CCAACAATCAAGTCTCTGACGCTAAGACTTCAAGACTATCCATCCTTGTCGCATATTGTTGTCTACGTAC
  CGTCTGTCCACGGAAGCAAGTTTGTTGTAGACTGTGAATTCTTTAAGAAAGAAGCAAGATCCATACAGCT
  TCCCGTAACCCACCTATTTTCTTTTGGATTGTCTTCCAGGAAGGCCATAATAAACACGAGCGGCCGGTAC
  TACAACATAGAGCTGCTGAACTTGGGACAGATATACCAAGCTTTTAAAGTCAACGTGGTCAGCAAGTGCA
  CTGGAGGCAAAGAAGAAATAACTAGTATCTATAAACTTCATATTCCCTGGTCTTATGAAGATTCTCTAAC
  CATTGCACAGGTTCCATCTTCCGTAGACATTTCTCTGAAGCTTCATGTTGCTCAGCCAGAAAATGACAGC
  CATGTGGCACTGTTAAAGATGTACACATCCTCAGACTGTCAGTATGAGGTGACGATCAAGACTTCCTTTC
  CACAAATACTGGGACAGGTAGTTCGATTTCATGGTGGAGCTCTTCCTGCCTACGTCGTCTCTAGCATCCT
  TCTGGCCTACGGAGGACAGTTATACTCTCTCCTCTCAACAGGTTTTTGCTTAGAATATGGTACCATGTTG
  GATAAAGAAGCCAAACCATACAAAGTTGACCCATTCGTAATTATGATTAAGTTTTTGTTGGGGTATAAAT
  GGTTTAAGGAATTATGGGATGCAGTGTTATTACCGGAATTAGATGCCATCGTTTTGACTAGTCAGAGTAT
  GTGTTTCCCTCTGGTGTCCCTGATCCTCTTCCTGTTCGGAACGTGTACTGCGTACTGGAGTGGTCTGCTC
  TCTTCCGCATCCGTACAGCTTCTTTCCTCATTGTGGCTGGCTCTGAAAAGACCTGCTGAACTTCCTAAGG
  ATGTCAAAGTGATGTCACCAGACCTGCCCGTTTTGACAGTTGTCTTCCTCATAATCAGCTGGACGACTTG
  CGGGGCACTTGCCATACTTCTGTCTTACCTGTACTATGTGTTTAAGGTTGTTCATCTGCAAGCCAGCCTA
  ACAACATTTAAAAATAATCAGCCAGTGAATCCCAAACACTCCAGAAGAAGTGAGAAAAAGTCCAACCACC
  ATAAAGATTCTGCCATACAGAATCCCCGCTTATCTGCTAATGATGCTGAAGACAGCCTTCGCATGCACAG
  CACCGTGATTAACTTGCTGACGTGGGTCGTGCTGCTCAGCATGCCGTCCCTGATCTACTGGTCAAAGAAC
  CTTAGATATTACTTTAAACTTAATCCTGACCCATGCAAACCCCTGGCGTTCCTGCTTATTCCAGCAATAG
  CGGTTCTTGGAAACACTCACACAGTTTCAATAAAGTCAAGTAAATTGCTGAAGACCGCCTCCCAATTCCC
  ACTTCCTCTGGCTGTCGGCGTGATCGCTTTTGGCTCCTCCCACTTATACAGGGTCCCGTGTTTTGTGATC
  ATTCCTCTTGTGTTCCACTCATTATGCAACTTCATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_201990
• Insert Size: 2769 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201990.1, NP_973719.1
• RefSeq Size: 3260 bp
• RefSeq ORF: 2769 bp
• Locus ID: 316400
• UniProt ID: Q765A7
• Cytogenetics: 9q31
• Gene Summary: Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi.[UniProtKB/Swiss-Prot Function]