Dnajb6 (NM_001013209) Rat Untagged Clone

CAT#: RN208753

Dnajb6 (untagged ORF) - Rat DnaJ (Hsp40) homolog, subfamily B, member 6 (Dnajb6), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dnajb6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208753 representing NM_001013209
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATTACTATGAAGTGCTGGGCGTGCAGAGACATGCCTCACCTGAGGACATTAAAAAGGCGTATC
  GAAAACAGGCACTCAAATGGCACCCAGACAAAAATCCTGAAAATAAAGAAGAAGCGGAGCGGAAATTCAA
  ACAAGTAGCTGAGGCATATGAAGTGTTATCGGATGCTAAAAAGCGGGACATCTATGACAAATATGGCAAA
  GAAGGATTAAATGGTGGTGGAGGAGGAGGTGGAAGTCATTTTGACAGTCCATTTGAGTTTGGCTTCACAT
  TCCGGAACCCAGATGATGTCTTCAGGGAATTTTTTGGTGGAAGGGACCCATTTTCATTTGACTTCTTTGA
  AGATCCATTTGATGACTTTTTTGGAAACCGAAGGGGTCCCCGAGGAAGTAGAAGCCGAGGTGCTGGCTCC
  TTTTTCTCCGCCTTCAGTGGATTTCCTTCTTTTGGAAGTGGATTTCCTGCTTTTGATACAGGCTTTACTC
  CATTCGGGTCACTAGGTCATGGGGGTCTCACTTCATTTTCTTCAGCGTCATTTGGCGGCAGTGGAATGGG
  CAACTTCAAATCAATATCAACTTCAACTAAAATAGTTAATGGCAAAAAAATCACTACAAAGAGAATTGTG
  GAGAACGGTCAGGAAAGAGTAGAAGTTGAAGAAGACGGACAGTTAAAGTCCCTGACGATAAATGGTGTGG
  CCGACGAGAACGCCCTTGCAGAAGAATGCCGGCGGAGAGGCCAACCCACACCAGCTCTGGCCCCTGGCCC
  GGCCCCAGCACCAGCCCGGGTCCCCTCTCAGGCCCGACCCCCGACCCCAGCTCCCACCCCAGCTCCAGCC
  CAGACTCCAGCCCCTTCTGTCAGCACCCGCCCACAGAAGCCACCCAGGCCTGCCCCAACGGCCAAACTTG
  TGTCTAAAAGCAACTGGGAAGATGAAGAGCAGGACAGGCAGCGGGTTCCTGGCAACTGTGATGCCCCTAT
  GACCTCAGCAGGGCTCAAAGAAGGTGGCAAGAGGAAGAAGCAGAAGCAGAAAGAGGACTCGAAGAAGAAG
  AAGTCGACCAAAGGGAACCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013209
• Insert Size: 1074 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013209.1, NP_001013227.1
• RefSeq Size: 2474 bp
• RefSeq ORF: 1074 bp
• Locus ID: 362293
• UniProt ID: Q6AYU3
• Cytogenetics: 4q11
• Gene Summary: Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).[UniProtKB/Swiss-Prot Function]