Tbx6 (NM_001108920) Rat Untagged Clone

CAT#: RN208413

Tbx6 (untagged ORF) - Rat T-box 6 (Tbx6), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tbx6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208413 representing NM_001108920
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCATCCACGAGAGTTATACCCCTCTCTGGGGACTGGCTACCGTCTGGGACACCCCCAGCCTGGGG
  CAGACTCAACCTTCCCACCTGCCCTGACAGAGGGCTACCGCTACCCTGATTTGGATACTTCTAAACTGGA
  TTGCTTCCTCTCTGGGATTGAGGCAGCTCCCCACACTCTGGCTGCACCCCCTCCTTTGCCCCTTCTCCCA
  TCTGCTCTGGGCCCTGAGACAGCACCGCCACCCCCAGAGGCCCTTCACTCGCTTCCTGGGGTCAGCCTGA
  GCTTGGAGAACCAGGAACTGTGGAAGGAATTCAGCGCTGTGGGAACAGAGATGATCATCACCAAGGCTGG
  CAGGCGTATGTTCCCTGCTTGCCGAGTATCAGTCACAGGCCTGGACCCGGAGGCCCGGTACTTGTTCCTT
  CTGGATGTGGTTCCAGTGGATGGGGCCCGATACCGCTGGCAGGGCCAGCACTGGGAGCCCAGTGGCAAGG
  CTGAGCCCCGCCTACCCGACCGTGTCTACATTCACCCTGATTCTCCTGCCACTGGTGCCCACTGGATGCG
  GCAGCCCGTATCCTTCCACCGTGTTAAGCTCACCAACAGCACGCTGGACCCCCATGGCCACCTGATCTTG
  CATTCCATGCACAAGTACCAGCCTCGCATTCACCTGGTGAGAGCCACCCAACTCTGCAGCCAACACTGGG
  GGGGTGTGGCCTCCTTCCGATTTCCTGAGACCACATTCATCTCAGTGACAGCCTACCAGAACCCTAGGAT
  CACACAGCTGAAGATCGCAGCCAATCCCTTTGCCAAAGGTTTCCGAGAAAATGGCAGAAACTGTAAGAGG
  GAGCGGGATGCCCGTGTGAAGAGGAAACTCCGGGGCCCAGAGCCAGTGGCCACAGAGGCCTGTGGGAGTG
  GGGATACACCAGGGGGTCCCTGTGACTCCACCCTGGGTGGGGATATTCGAGATTCAGATCCAGAGCCGGC
  TCCTACCCCCCAGGAAGCTGCTTCTGCCTCAGCTCCTCCATGTGGGGGCCCCAGTGCTGAGGCCTACCTC
  CTGCACCCTGCAGCTTTTCATGGGGCCCCCAGTCATCTACCAGCCAGGACCCCCAGCTTCCCTGAGGCTC
  CAGACCCTGGGCGCCCAGCCCCCTACTCAGCTGCATTCCTGGAGCTACAGCCTGGACCGGGGAGCTCTGC
  TTATCAAACGACCCCATCTGTAACACCCTTTGCCCCTCACTTTATTCAAGGGGCCCCCTTCCCTCTACCA
  TACCCAGGACCTGGAGGATATCTGGACATGGGATCCAAGCAAATGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001108920
• Insert Size: 1311 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108920.1, NP_001102390.1
• RefSeq Size: 1726 bp
• RefSeq ORF: 1311 bp
• Locus ID: 365371
• UniProt ID: D3ZJK7
• Cytogenetics: 1q36
• Gene Summary: T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to also play an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia (By similarity).[UniProtKB/Swiss-Prot Function]