Cyb5r4 (NM_133427) Rat Untagged Clone

CAT#: RN207642

Cyb5r4 (untagged ORF) - Rat cytochrome b5 reductase 4 (Cyb5r4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cyb5r4
• Synonyms: b5&b5R; b5+b5R; cb5/cb5R; Ncb5or
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207642 representing NM_133427
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACGTCCCTTCCCAGGCTTTCCCGGCCCCCGGCTCCCAGCAGCGCGTTGCCTCCCAGGGGCGTA
  GCAAGGTACCTTTAAAACAAGGCAGGAGTCTTATGGATTGGATCCGATTGACCAAAAGTGGAAAGGACTT
  CACGGGATTGAAGGGCGGGTTAATTGAAGTAACAGAAGAAGAACTTAAGAAGCACAACAAGAAAGACGAC
  TGTTGGATATGCATAAGAGGGTTTGTTTACAATGTCAGCCCATATATGGAGTATCATCCTGGTGGGGAGG
  ATGAGCTTATGAGAGCCGCAGGAGCAGATGGAACGGATCTTTTTAATGAGGTGCATCGTTGGGTCAATTA
  TGAATCCATGCTGAAAGAGTGCCTGGTTGGCAGGATGGCTGTGAAGCCCGCTGTTCCGAAAGACTGTCAT
  GAAGGAAAGAGAGTCTTAAATGGCATGCTTCCCAAGAGCCAAGTGACAGACACACTGCCCAGGGAAGGCC
  CCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCAGTGACCATTGTTATATACACCAAGCAGAA
  GAATATCAATTTAGACTCAGTAATCGTTGACCTCCAGGATGACTCCTTGAGAGCAGAAGCAGTCATCAAG
  GATCATTCTTATCTTATACATATTGGGTTAAGCCATGAAGTTCAAGAAAATTTTTCTGTACGGGTTATTG
  AGAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAAACTGTTTCTTGGAAATGTCTTGGTGATCC
  CCTGGAGAAACATGATTCTTTTATTCCAAAGAAAGATACAGGCTTGTACTATAGACAGTGCCAGTTAATT
  TCCAAGGAAGACGTTACTCATGACACAAGGCTCTTCTGTCTGATGCTGCCTCCCAGTACTCACCTTCAGG
  TGCCAGTTGGGCAACATGTTTACCTCAAGCTCAGTGTCACAGGTGCAGAAATAGTAAAGCCATATACACC
  CGTGTCTGAGTCCTTACTCTCAGATTTTAAAGAACCAGTTCTTTCCCCAAATAAGTACATCTACTTTTTG
  ATCAAAATCTATCCTGCTGGACTCTTCACGCCAGAACTTGATCGTCTTCAGATTGGAGATTTTGTTTCTG
  TAAGTGGTCCTGAGGGCAATTTTAAAGTTTCCAAACTCCAGGAAGTGGAAGATCTCTTTTTATTGGCAGC
  CGGAACAGGCTTCACACCAATGGTTACTGTACTGAATCACGCTTTAACTCACATGTCCAGTCTCAGGAAG
  GTGAAGTTAATGTTCTTCAATAAAACAGAGGATGACATAATTTGGAGATGCCAGCTAGAGAAATTGGCAT
  TAAAAGATAAAAGATTTCATGTTGAATATGTTCTCTCGGCACCTTCCCCAGAATGGAATGGCAAACAGGG
  ACACGTGTCACGAGCTCTTCTCTCTGAATTTTTGCAACGAAGTTTAGAAAATTCTAAAGTCTTCCTTTGC
  ATCTGTGGACCAACCCCATTTACAGATGAGGGGATAAGGTTGCTGCATGATCTTAACTTTTCCGATGATG
  AGATTCATGGTTTTACAGCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133427
• Insert Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133427.3, NP_596918.3
• RefSeq Size: 2252 bp
• RefSeq ORF: 1563 bp
• Locus ID: 171015
• UniProt ID: Q68EJ0
• Cytogenetics: 8q31
• Gene Summary: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).[UniProtKB/Swiss-Prot Function]