Nrf1 (NM_001100708) Rat Untagged Clone

CAT#: RN204818

Nrf1 (untagged ORF) - Rat nuclear respiratory factor 1 (Nrf1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nrf1
• Synonyms: Nfe2l1_retired
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204818 representing NM_001100708
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACACGGAGTGACCCAAACCGAACACATGGCTACCATAGAAGCCCATGCAGTGGCCCAGCAAG
  TTCAGCAGGTCCATGTAGCCACTTACACTGAGCACAGTATGCTAAGTGCTGATGAAGACTCCCCTTCCTC
  GCCTGAGGACACTTCTTACGATGACTCGGACATACTCAACTCCACGGCAGCTGATGAGGTAACTGCCCAT
  CTGGCTGCTGCAGGTCCTGTGGGAATGGCTGCTGCTGCTGCTGTGGCAACAGGGAAGAAACGGAAAAGGC
  CTCATGTGTTTGAGTCTAACCCATCTATCCGAAAGAGACAGCAGACACGGTTGCTTCGGAAACTCAGAGC
  CACATTAGATGAATATACGACACGAGTGGGACAGCAAGCCATTGTTCTCTGCATCTCACCCTCCAAACCC
  AACCCTGTCTTTAAGGTGTTTGGCGCAGCACCTTTGGAGAATGTGGTGCGCAAGTACAAGAGCATGATCC
  TGGAAGACCTGGAGTCTGCGCTGGCAGAGCACGCCCCTGCGCCACAGGAGGTTAATTCAGAACTGCCCCC
  TCTCACCATCGATGGGATTCCAGTCTCTGTGGACAAGATGACCCAGGCTCAGCTTCGGGCATTTATCCCA
  GAGATGCTCAAGTACTCCACAGGTCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCTATCTGGT
  GGCCAGAAGACATCCCATGGGCCAATGTCCGCAGTGACGTCCGCACAGAAGAGCAAAAGCAAAGGGTTTC
  ATGGACCCAAGCATTACGGACCATAGTTAAAAATTGTTATAAGCAGCATGGGCGGGAGGACCTTCTGTAT
  GCTTTCGAAGATCAGCAGACACAAACTCAGGCCACCACCACACACAGCATAGCCCATCTCGTACCATCAC
  AGACAGTAGTACAGACCTTCAGCAACCCTGATGGCACCGTGTCGCTCATCCAGGTTGGTACTGGGGCAAC
  AGTAGCCACATTGGCTGATGCTTCAGAACTGCCAACCACAGTCACTGTTGCCCAAGTGAATTATTCTGCT
  GTGGCTGATGGAGAGGTGGAACAAAATTGGGCCACATTACAGGGCGGTGAAATGACCATCCAGACGACGC
  AAGCATCAGAGGCCACCCAGGCAGTGGCATCACTGGCAGAGGCCGCAGTGGCCGCTTCTCAGGAGATGCA
  ACAGGGAGCCACTGTCACCATGGCCCTTAACAGTGAAGCTGCCGCCCATGCTGTCGCCACTCTGGCTGAA
  GCCACCTTACAAGGCGGGGGACAGATAGTCCTGTCTGGGGAAACCGCAGCAGCCGTTGGAGCACTTACTG
  GAGTCCAAGATGCTAATGGCCCAGATGGAGTTCATGCATGGACCATCAGCAAAGCCGTGACGGCTCCTCA
  GGCTGCAGGAAAAATTCATACAGATTTTGGAAAGGGATTCATTATGGCGGAAGTAATGAAAGACGAAGAT
  CTTAAAGAGGAGAGTTCTGAGAACGCAGCTAAGGCTGCTGGGAAGTACAAACAACCAGGCAGAGCTTACG
  TTGTTGAAGACGGAGATATTATCTTCTTCAAATTTAACTCGCCACAGCAACTGAAAAGGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001100708
• Insert Size: 1605 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100708.1, NP_001094178.1
• RefSeq Size: 1898 bp
• RefSeq ORF: 1605 bp
• Locus ID: 312195
• Cytogenetics: 4q22
• Gene Summary: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Confusion has occurred in bibliographic databases for genes in the "nuclear respiratory factor" and "nuclear factor (erythroid-derived 2)-like" families due to a shared symbol or alias of Nrf1. [provided by RefSeq, Jul 2008]