Vcl (NM_001107248) Rat Untagged Clone

CAT#: RN203691

Vcl (untagged ORF) - Rat vinculin (Vcl), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Vcl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203691 representing NM_001107248
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGTTTCACACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCTCACCTGG
  TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCTGT
  GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGGAAAGAGACCGTTCAGACCACTGAGGATCAGATTTTG
  AAGAGAGATATGCCGCCAGCATTTATTAAGGTCGAGAATGCTTGCACGAAGCTTGTTCAGGCAGCTCAGA
  TGCTTCAGTCAGACCCATACTCTGTTCCTGCGCGGGATTACCTCATCGACGGCTCCAGGGGAATCCTCTC
  TGGAACGTCTGACCTCCTGCTTACCTTTGATGAGGCTGAGGTTCGAAAAATTATAAGAGTTTGCAAAGGG
  ATTTTGGAATACCTCACAGTGGCAGAGGTAGTGGAGACTATGGAAGACTTGGTCACTTACACAAAGAACC
  TTGGGCCCGGAATGACGAAGATGGCCAAAATGATCGATGAGAGACAGCAGGAGTTGACGCACCAGGAACA
  CCGTGTGATGCTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG
  ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTCTGAAAAATCGAAATTTTACTG
  TAGGAAAGATGAGTGCTGAGATTAACGAGATCATTCGTGTGTTACAACTCACCTCCTGGGATGAGGACGC
  CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCCTCCATAGACTCGAAACTGAACCAGGCC
  AAAGGTTGGCTCCGTGACCCCAACGCTTCCCCAGGGGACGCCGGTGAGCAGGCCATCAGGCAGATCTTGG
  ACGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAGAT
  GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCCTCGCCAGTGGCCATG
  CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACTGCGAAAGTGGAAAATGCAGCTCGAAAGC
  TGGAAGCCATGACGAACTCAAAGCAGAGTATTGCGAAGAAGATTGACGCTGCGCAGAATTGGCTGGCAGA
  TCCCAATGGTGGACCTGAAGGAGAAGAACAGATCCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCTGAG
  TTATGTGATGATCCTAAGGAGAGGGACGACATCCTCCGCTCTCTTGGAGAGATAGCGGCTCTGACCTCTA
  AATTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCCCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC
  AACCGCGCTACAGAACCTGCAGACCAAAACCAATAGGGCTGTGGCCAACAGCAGGCCTGCCAAGGCAGCC
  GTCCACCTGGAAGGCAAGATTGAGCAGGCACAGCGGTGGATTGATAACCCTACAGTGGATGACCGAGGAG
  TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAAGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA
  TCGCCAAGATCTTCTCGCCAAGTGTGACCGTGTAGACCAGCTAGCAGCTCAGCTGGCTGACCTGGCTGCC
  CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCGCTTGCTTCTCAGCTTCAGGACTCCTTAAAGGATCTTA
  AAACCCAGATGCAGGAAGCTATGACTCAAGAGGTGTCCGACGTTTTCAGCGATACTACAACTCCCATCAA
  GCTGTTGGCAGTGGCGGCCACTGCTCCTCCTGATGCGCCCAATAGGGAAGAGGTATTTGACGAGAGAGCA
  GCCAACTTTGAAAACCATTCAGGAAGGCTTGGGGCCACGGCAGAGAAGGCAGCTGCTGTTGGTGCTGCCA
  ATAAATCGACAGTGGAAGGCATTCAGGCATCCGTGAAGACAGCCCGAGAACTCACTCCCCAGGTGATCTC
  AGCTGCTCGAATCTTACTGAGGAACCCTGGTAACCAAGCTGCGTATGAACATTTTGAGACCATGAAGAAC
  CAGTGGATTGATAATGTTGAGAAAATGACAGGGCTGGTGGACGAGGCCATTGATACCAAATCTCTGTTGG
  ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA
  GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG
  GTAGAGAACTCTGAGGATCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA
  TCTCCCCCATGGTTATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT
  CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAAGTTAGAGAAGCCTTCCAACCTCAGGAGCCT
  GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGTCTAACTGATGAGCTGGCTCCTCCCAAACCAC
  CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCACCCCCACCAGAAGAGAAGGATGAAGAGTTCCCTGA
  GCAGAAAGCCGGGGAGGTGATTAACCAGCCGATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG
  AAGTGGTCTAGCAAGGGCAATGACATCATCGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCGGAGATGT
  CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC
  CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT
  CTCTTACAGGTCTGTGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCA
  CCATGCTGGGCCGGACCAACATCAGTGACGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC
  CCAGAACCTCATGCAGTCTGTGAAAGAGACTGTGCGAGAGGCCGAAGCTGCTTCAATCAAGATTCGAACA
  GACGCCGGATTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001107248
• Insert Size: 3201 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107248.1, NP_001100718.1
• RefSeq Size: 5270 bp
• RefSeq ORF: 3201 bp
• Locus ID: 305679
• UniProt ID: P85972
• Cytogenetics: 15p16
• Gene Summary: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.[UniProtKB/Swiss-Prot Function]