Chia (NM_207586) Rat Untagged Clone

CAT#: RN201701

Chia (untagged ORF) - Rat chitinase, acidic (Chia), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Chia
• Synonyms: MGC112572
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201701 representing NM_207586
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGCTCATTCTTGTCACAGGTCTGGTTCTTCTGCTGAATGTTCAGCTGGGGTCTGCCTACAATC
  TGGTATGCTACTTCACCAACTGGGCCCAGTATCGGCCAGGCCTGGGGAGTTTCAAGCCTGATGACATTAA
  TCCCTGCCTGTGTACTCACCTGATCTATGCCTTTGCTGGGATGCAGAACAATCAAATCACCACCATAGAA
  TGGAATGATGTTACTCTCTATAAAGCTTTCAATGACTTGAAAAACAGGAACAGCAAACTGAAAACTCTCC
  TGGCAATTGGAGGTTGGAACTTTGGAACTGCTCCTTTCACCACCATGGTTTCCACTTCTCAGAACCGCCA
  GACCTTTATTACCTCAGTCATCAAATTCCTGCGACAGTATGGGTTTGATGGACTGGACCTGGACTGGGAA
  TACCCAGGTTCACGTGGGAGCCCTCCTCAGGACAAGCATCTCTTCACTGTCCTGGTGAAGGAACTACGTG
  AAGCTTTTGAGCAGGAGGCTATTGAGAGCAACAGGCCCAGATTGATGGTTACTGCTGCTGTAGCTGCTGG
  CATTTCCAACATCCAGGCTGGCTACGAGATCCCTGAACTTTCTCAGTACCTGGATTTCATCCACGTCATG
  ACATATGACCTCCATGGCTCCTGGGATGGCTACACTGGGGAGAACAGTCCTCTTTACAAACTCCCTACTG
  AGACTGGCAGCAATGCCTACCTCAATGTGGATTATGTCATGAATTACTGGAAGGATAATGGAGCCCCAGC
  TGAGAAGCTCATTGTTGGATTTCCAGAGTATGGACACACCTACATCCTAAGCAACCCTTCTGATACTGGA
  ATTGGTGCCCCTACCTCTGGTAATGGCCCTGCTGGGCCCTATACCAGGCAGGCTGGGTTCTGGGCCTACT
  ATGAGATTTGTACCTTTCTGAGAAATGGAGCCACTCAGGACTGGGATGCCCCCCAGGAAGTTCCCTATGC
  CTACAAGGGCAATGAGTGGGTTGGCTATGATAATATCAAGAGCTTCAGTGTCAAGGCTCAGTGGCTTAAG
  CAGAACAACTTTGGTGGTGCCATGATCTGGGCCATTGACCTTGATGACTTCACTGGCTCTTTCTGTGACC
  AGGGAAAATTCCCTCTGACTTCTACTTTGAATAAAGCCCTTGATATACCCACAGCAGGTTGCACAGCCCC
  TGACTTACCTTCTGAACCAGTAACTACTCCTCCAGGAAGTGGGAGTGGTGGTGGAAGCTCTGGTGGAGGT
  TCTGAAGGCAGTGGATTCTGTGCTGGCAAAGCAGATGGCCTCTACCCTGTGGCAGATGACAGAAATGCTT
  TCTGGCACTGCATCAATGGAATCACATATCAGCAGCATTGTCAGGCAGGGTTAGTTTTTGATACCAGCTG
  TAATTGCTGCAACTGGCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207586
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207586.2, NP_997469.1
• RefSeq Size: 1538 bp
• RefSeq ORF: 1422 bp
• Locus ID: 113901
• UniProt ID: Q6RY07
• Cytogenetics: 2q34
• Gene Summary: Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding (By similarity).[UniProtKB/Swiss-Prot Function]