Camk2b (NM_001042354) Rat Untagged Clone

CAT#: RN200521

Camk2b (untagged ORF) - Rat calcium/calmodulin-dependent protein kinase II beta (Camk2b), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Camk2b
• Synonyms: Ck2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200521 representing NM_001042354
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACGGTGACCTGCACCCGTTTCACGGACGAGTACCAGCTATACGAGGATATTGGCAAGGGGG
  CTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGCACCGGCCATGAGTATGCAGCTAAGATCATTAACAC
  CAAGAAGCTGTCAGCTAGAGATCACCAGAAGCTGGAGAGGGAGGCTCGGATCTGCCGCCTGCTGAAGCAT
  TCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCTTCGACCTGGTCA
  CTGGTGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGTGAGGCTGACGCCAGTCACTGTAT
  CCAGCAGATCCTGGAGGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCTGAA
  AACCTGCTCCTGGCCAGCAAATGCAAAGGGGCCGCAGTGAAACTGGCAGACTTCGGCCTGGCCATCGAGG
  TTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACACCAGGCTACCTGTCTCCCGAAGTTCTTCG
  GAAGGAGGCCTATGGCAAACCAGTGGATATCTGGGCATGTGGGGTGATCCTGTATATCCTGCTGGTGGGA
  TACCCACCTTTCTGGGATGAGGACCAGCACAAGCTGTACCAGCAGATCAAGGCTGGGGCCTATGACTTCC
  CATCCCCCGAGTGGGACACCGTTACCCCTGAAGCCAAAAACCTCATCAACCAGATGTTGACCATCAACCC
  CGCCAAGCGCATCACGGCCCACGAGGCCCTGAAGCACCCATGGGTCTGCCAACGATCCACGGTGGCCTCC
  ATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGCTCAAGGGAGCCA
  TCCTCACCACTATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGACGG
  AGTCAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCCCTCCCTCCT
  GCCGCCCTGGAATCTTCCGACAGCACCAACACAACCATAGAGGACGAAGATGCCAAAGCCCCCAGGATCT
  CTGACATCCTGAACTCAGTGAGAAGGGGCTGTGGAACCCCAGAAGCCGAGGGACCCCTATCAGTGGGGCC
  CCCGCCCTGCTTGTCTCCGGGTCTCCTAGGCCCCCTGCCCACCCCGTCCCCCAGGATCTCTGACATCCTC
  AACTCAGTGAGGCGGGGCTCAGGGACCCCAGAAGCTGAGGGCCTCCCACCAGTGGGCCCCCCACCCTGCC
  CATCTCCGACTCTCCCTGGCCCCTTGCCCACCCCATCCCGGAAGCAGGAAATCATCAAGACCACAGAGCA
  GCTCATCGAGGCCGTCAACAACGGCGACTTTGAGGCCTATGCGAAAATCTGTGACCCAGGCCTGACCTCA
  TTTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAGATTCTACTTTGAGAACCTGC
  TGGCCAAGAACAGCAAGCCGATCCACACCACTATCCTGAACCCGCACGTGCACGTCATCGGCGAGGATGC
  AGCCTGCATCGCTTACATCCGCCTCACACAGTACATCGACGGCCAGGGCAGACCCCGCACCAGCCAGTCC
  GAAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTCCATTTCCACTGCTCGGGCGCTC
  CAGTGGCCCCACTGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042354
• Insert Size: 1770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001042354.1, NP_001035813.1
• RefSeq Size: 4005 bp
• RefSeq ORF: 1770 bp
• Locus ID: 24245
• Cytogenetics: 14q21
• Gene Summary: beta subunit of calcium/calmodulin-dependent protein kinase II; may be involved in retinal development [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).