ZNF281 (NM_001281294) Human Tagged ORF Clone

CAT#: RG239697

ZNF281 (tGFP-tagged) - Human zinc finger protein 281 (ZNF281), transcript variant 3

Product Images

Circular map for RG239697

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF281
• Synonyms: GZP1; ZBP-99; ZBP99; ZNP-99
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239697 representing NM_001281294.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAATCGGCAGTGGGTTCCTGAGTGGCGGCGGAGGTACCGGCAGTAGCGGTGGTAGCGGCTCCGGC
  GGCGGTGGTAGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGGAGGGCAGAGATGGAACCCACCTTT
  CCCCAGGCCCCGGCCGCTGAGCCCCCCCCTCCGCCAGCCCCGGACATGACTTTCAAGAAGGAGCCGGCG
  GCGTCAGCCGCGGCCTTCCCCTCGCAGAGGACCTCCTGGGGGTTCTTGCAGTCTTTGGTTAGCATCAAA
  CAGGAGAAACCCGCGGATCCTGAGGAGCAGCAGTCCCACCACCACCATCACCACCACCACTATGGGGGG
  CTGTTCGCTGGAGCTGAAGAGAGGTCTCCAGGCCTAGGAGGCGGTGAAGGGGGGAGTCACGGCGTCATC
  CAGGACCTCAGTATTCTCCACCAGCATGTCCAGCAGCAACCAGCCCAGCACCACCGTGACGTATTACTC
  AGCAGCAGTAGCAGGACTGATGACCACCATGGCACTGAGGAGCCAAAGCAGGACACTAATGTCAAAAAG
  GCAAAAAGGCCAAAGCCAGAATCTCAGGGAATCAAAGCCAAGAGGAAGCCAAGTGCATCTTCCAAACCT
  TCTTTGGTTGGAGATGGAGAAGGTGCCATCCTCTCCCCAAGTCAGAAACCTCATATCTGTGATCACTGT
  AGTGCTGCTTTCCGAAGCTCCTATCACCTGCGGAGACATGTCCTCATTCATACAGGAGAAAGACCTTTC
  CAGTGCAGCCAGTGTAGTATGGGTTTCATTCAGAAATACCTACTACAGAGACATGAGAAAATTCATAGT
  AGAGAGAAGCCATTTGGATGTGATCAGTGCAGCATGAAGTTTATTCAGAAGTACCATATGGAGAGACAC
  AAGAGGACACATAGTGGAGAAAAGCCATATAAGTGTGACACTTGCCAACAGTATTTTTCAAGGACTGAT
  AGATTGTTGAAGCACAGGCGCACATGTGGTGAAGTCATAGTTAAAGGAGCCACTAGTGCAGAACCTGGG
  TCATCAAACCATACCAATATGGGTAATCTGGCTGTGTTGTCTCAGGGAAATACAAGTTCTTCAAGGAGA
  AAAACAAAGTCAAAAAGCATAGCTATTGAAAATAAGGAACAGAAGACCGGTAAAACAAATGAATCGCAA
  ATTTCAAATAATATAAACATGCAGAGTTACTCAGTAGAAATGCCTACCGTGTCTTCCAGTGGAGGCATA
  ATTGGCACTGGAATAGATGAACTGCAGAAGAGGGTGCCAAAATTGATCTTTAAGAAAGGAAGCAGAAAG
  AATACAGATAAAAACTACCTTAACTTTGTGTCACCATTACCAGACATAGTAGGACAGAAATCCTTGTCT
  GGAAAACCAAGTGGCTCACTTGGCATAGTATCAAATAATAGTGTGGAGACCATTGGTCTTCTCCAAAGT
  ACAAGTGGCAAACAAGGTCAGATAAGTAGTAATTATGATGATGCCATGCAGTTTTCAAAGAAAAGAAGA
  TATTTACCAACTGCCAGCAGCAACAGTGCCTTTTCTATAAACGTAGGACACATGGTCTCCCAACAGTCT
  GTCATTCAGTCTGCAGGTGTCAGTGTTTTGGACAATGAGGCACCATTGTCACTTATTGACTCCTCAGCT
  CTAAATGCTGAAATTAAATCTTGTCATGACAAGTCTGGAATTCCTGATGAGGTTTTACAAAGTATTTTG
  GATCAATACTCCAACAAATCAGAAAGCCAGAAAGAGGATCCTTTCAATATTGCAGAACCACGAGTGGAT
  TTACACACCTCAGGAGAACACTCAGAATTGGTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACACCT
  TCAAATGATAAAGCAAGTATGTTGCAAGAATACTCCAAATACCTCCAACAGGCTTTTGAAAAATCCACT
  AATGCAAGTTTTACTCTTGGACACGGTTTCCAATTTGTCAGTTTGTCTTCACCTCTCCACAACCACACT
  TTGTTTCCAGAAAAACAAATATACACTACGTCTCCTTTGGAGTGTGGTTTCGGCCAATCTGTTACCTCA
  GTGTTGCCATCTTCATTGCCAAAGCCTCCTTTTGGGATGTTGTTTGGATCTCAGCCAGGTCTTTATTTG
  TCTGCTTTGGATGCTACACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAG
  AAGAACTTAGAGACTTCATCAGCCTTCCAGTCCTCATCTCAGAAATTGACTAGCCAGAAGGAACAGAAA
  AACTTAGAGTCTTCAACAGGCTTTCAGATTCCATCTCAGGAGTTAGCTAGCCAGATAGATCCTCAGAAA
  GACATAGAGCCTAGAACAACGTATCAGATTGAGAACTTTGCACAAGCGTTTGGTTCTCAGTTTAAGTCG
  GGCAGCAGGGTGCCAATGACCTTTATCACTAACTCTAATGGAGAAGTGGACCATAGAGTAAGGACTTCA
  GTGTCAGATTTCTCAGGGTATACAAATATGATGTCTGATGTAAGTGAGCCATGTAGTACAAGAGTAAAG
  ACACCCACCAGCCAGAGTTACAGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239697
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKIGSGFLSGGGGTGSSGGSGSGGGGSGGGGGGGSSGRRAEMEPTFPQAPAAEPPPPPAPDMTFKKEPA
  ASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQQSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVI
  QDLSILHQHVQQQPAQHHRDVLLSSSSRTDDHHGTEEPKQDTNVKKAKRPKPESQGIKAKRKPSASSKP
  SLVGDGEGAILSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHS
  REKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEVIVKGATSAEPG
  SSNHTNMGNLAVLSQGNTSSSRRKTKSKSIAIENKEQKTGKTNESQISNNINMQSYSVEMPTVSSSGGI
  IGTGIDELQKRVPKLIFKKGSRKNTDKNYLNFVSPLPDIVGQKSLSGKPSGSLGIVSNNSVETIGLLQS
  TSGKQGQISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSA
  LNAEIKSCHDKSGIPDEVLQSILDQYSNKSESQKEDPFNIAEPRVDLHTSGEHSELVQEENLSPGTQTP
  SNDKASMLQEYSKYLQQAFEKSTNASFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTS
  VLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQK
  NLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTS
  VSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281294
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001281294.2
• RefSeq Size: 4800 bp
• RefSeq ORF: 2580 bp
• Locus ID: 23528
• UniProt ID: Q9Y2X9
• Cytogenetics: 1q32.1
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors
• MW: 93.7 kDa
• Gene Summary: Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function]