METTL9 (NM_001288659) Human Tagged ORF Clone

CAT#: RG237119

METTL9 (tGFP-tagged) - Human methyltransferase like 9 (METTL9), transcript variant 3

Product Images

Circular map for RG237119

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: METTL9
• Synonyms: CGI-81; DREV; DREV1; PAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237119 representing NM_001288659.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATCGAAAGACCACGAGCTGGGATACCAAGTTCTATGAAAAGTGGTATGTGTGCAACAGAGAGAAA
  TTATGCGAATCACTCCAGGCTGTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAAC
  AACAGCATTGAGAAATCGGGCTGGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGC
  CTGTTTATGTCTAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGAT
  CAGTTTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGA
  GATGGAGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACT
  ATGATATGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTC
  CAGTATGATGTCATCAGCTGCCTGAACTTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGAT
  ATCAGAAGTGTCTTGGAGCCAACTAGAGGCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTAT
  GTGGAAAACGTAGGTGGCAAGTGGGAGAAACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAA
  GAACAAGTGAATAGTCTGCCTGAAGTTTTCAGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTA
  CCATACCTGTGTGAAGGCGACATGTATAATGACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAA
  CCAGTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237119
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDRKTTSWDTKFYEKWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFS
  LFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSET
  MIWQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPY
  VENVGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLK
  PV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288659
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001288659.1, NP_001275588.1
• RefSeq Size: 2924 bp
• RefSeq ORF: 837 bp
• Locus ID: 51108
• UniProt ID: Q9H1A3
• Cytogenetics: 16p12.2
• MW: 32.9 kDa
• Gene Summary: Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function]