OSBPL6 (NM_001201480) Human Tagged ORF Clone
CAT#: RG235080
- TrueORF®
OSBPL6 (tGFP-tagged) - Homo sapiens oxysterol binding protein-like 6 (OSBPL6), transcript variant 3
ORF Plasmid: DDK
"NM_001201480" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | OSBPL6 |
Synonyms | ORP6 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235080 representing NM_001201480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGATGAGAAGGGCATTTCCCCTGCTCATAAAACATCCACTCCAACCCATAGAAGTGCCTCCT CTTCAACATCCTCCCAAAGGGACAGTAGGCAGAGTATTCACATACTGGAGAGGACTGCTTCCTCTAGCAC CGAGCCCTCTGTAAGTCGGCAATTGCTAGAACCGGAGCCAGTCCCCCTCTCCAAGGAAGCTGACAGCTGG GAAATTATAGAAGGGCTGAAAATAGGCCAAACCAATGTCCAGAAACCAGACAAACATGAGGGCTTTATGC TGAAGAAAAGAAAATGGCCTTTAAAAGGCTGGCACAAGCGTTTTTTTGTCCTGGATAATGGAATGTTAAA GTATTCAAAGGCACCACTCGATATTCAGAAAGGAAAGGTCCATGGGAGCATAGATGTGGGACTCTCAGTC ATGTCAATTAAAAAGAAAGCTCGAAGAATAGACCTTGACACCGAAGAGCACATCTATCATTTGAAGGTGA AATCCCAGGACTGGTTTGATGCATGGGTCTCCAAACTGCGACATCATCGGTTGTATCGTCAGAATGAAAT TGTGAGATCACCAAGAGATGCTAGTTTTCACATATTTCCTTCAACGTCCACAGCTGAATCCTCACCAGCT GCTAATGTTTCTGTAATGGATGGAAAGATGCAACCAAACAGCTTTCCGTGGCAGTCCCCTTTACCATGCA GCAATAGCCTCCCTGCAACGTGCACAACTGGCCAGAGTAAAGTGGCAGCCTGGTTACAGGACTCGGAAGA GATGGACAGGTGTGCAGAAGATCTTGCACATTGCCAGTCAAACCTTGTGGAACTTAGCAAACTCCTGCAA AATTTGGAAATACTTCAGAGAACTCAGTCGGCACCTAACTTTACTGACATGCAGGCTAACTGTGTAGATA TTTCAAAGAAAGACAAGCGGGTCACAAGACGATGGAGAACAAAAAGTGTCAGCAAAGATACAAAAATACA ACTGCAGGAAGGGCCACCCGCGAAGGGCCAGTTCAGCACAACTCGGCGCCGGCAGAGGCTAGCGGCAGCA GTGGCTACAACAGTTCCTTTCAGTGCTACCATGTCACCAGTTCGCTTGCATTCCTCCAACCCCAACCTTT GTGCAGATATTGAATTTCAGACTCCCCCTAGCCACCTCACTGACCCTCTGGAAAGTTCAACAGATTATAC AAAGCTGCAAGAAGAATTTTGTCTAATCGCACAGAAAGTGCATTCTCTTTTGAAGTCTGCATTTAATAGC ATAGCTATAGAGAAGGAGAAGCTGAAGCAGATGGTTTCCGAGCAGGATCACAGTAAAGGCCACAGCACGC AGATGGCACGGCTCCGACAGTCACTGTCTCAGGCACTCAACCAGAATGCTGAACTAAGGAGTCGGTTGAA CAGAATACATTCAGAGTCTATTATTTGTGATCAGGTTGTCAGTGTAAATATTATTCCTAGCCCTGATGAG GCTGGTGAGCAAATCCATGTCAGTCTCCCCTTATCACAGCAAGTAGCCAATGAGAGCCGCCTCTCCATGT CAGAGTCTGTTTCTGAGTTCTTTGATGCCCAAGAGGTGCTCCTCTCTGCAAGTTCGTCAGAGAATGAGGC TTCAGATGATGAGTCTTACATCAGTGATGTGAGTGATAATATATCTGAAGACAACACCAGTGTTGCAGAC AATATTTCTCGGCAAATCCTGAATGGGGAGCTTACAGGAGGGGCCTTCCGAAATGGGCGTCGAGCATGCC TGCCAGCTCCTTGTCCTGACACCAGTAACATTAACCTGTGGAATATCTTGAGGAACAACATTGGTAAAGA CCTGTCTAAAGTCTCTATGCCTGTGGAGCTAAACGAGCCGCTCAACACCCTGCAGCACCTCTGTGAGGAA ATGGAATACAGCGAGCTCCTGGACAAGGCTTCGGAAACTGATGATCCATATGAGCGCATGGTTCTCGTTG CCGCATTTGCAGTTTCAGGATACTGCTCCACCTATTTCAGAGCAGGAAGTAAGCCATTCAACCCAGTCCT TGGGGAGACTTATGAATGCATTAGAGAAGACAAGGGATTCCGCTTTTTCTCAGAACAGGTTAGCCATCAT CCACCCATTTCTGCCTGTCACTGTGAATCAAAGAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAACA AGTTCTGGGGGAAGTCGATGGAAATCCTGCCTGTTGGAACACTGAATGTCATGCTTCCAAAGTATGGAGA TTACTATGTGTGGAATAAAGTCACCACTTGCATACACAACATCCTCAGTGGGAGAAGATGGATAGAACAT TATGGAGAAGTAACCATCAGAAATACCAAAAGCAGTGTTTGCATTTGCAAACTCACATTTGTCAAGGTGA ATTATTGGAATTCTAACATGAATGAAGTCCAGGGGGTGGTGATAGATCAGGAGGGGAAGGCGGTGTACCG GCTGTTTGGAAAGTGGCATGAAGGACTCTACTGTGGTGTGGCCCCCTCTGCAAAGTGCATTTGGAGACCA GGTTCCATGCCAACAAACTATGAGCTGTACTATGGCTTCACAAGGTTTGCTATTGAGCTCAATGAGTTAG ATCCAGTACTAAAAGATCTCCTTCCACCAACAGACGCCCGGTTCCGGCCAGATCAAAGATTTTTGGAAGA AGGAAATTTAGAAGCTGCAGCATCAGAGAAGCAAAGAGTAGAGGAACTCCAGAGATCTCGGAGACGATAT ATGGAAGAAAACAATCTTGAACATATACCAAAATTTTTTAAAAAAGTTATTGATGCCAATCAAAGAGAAG CCTGGGTTTCTAACGACACCTACTGGGAGCTTCGAAAGGACCCTGGGTTTAGCAAAGTAGACAGCCCTGT TCTTTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235080 representing NM_001201480
Red=Cloning site Green=Tags(s) MSSDEKGISPAHKTSTPTHRSASSSTSSQRDSRQSIHILERTASSSTEPSVSRQLLEPEPVPLSKEADSW EIIEGLKIGQTNVQKPDKHEGFMLKKRKWPLKGWHKRFFVLDNGMLKYSKAPLDIQKGKVHGSIDVGLSV MSIKKKARRIDLDTEEHIYHLKVKSQDWFDAWVSKLRHHRLYRQNEIVRSPRDASFHIFPSTSTAESSPA ANVSVMDGKMQPNSFPWQSPLPCSNSLPATCTTGQSKVAAWLQDSEEMDRCAEDLAHCQSNLVELSKLLQ NLEILQRTQSAPNFTDMQANCVDISKKDKRVTRRWRTKSVSKDTKIQLQEGPPAKGQFSTTRRRQRLAAA VATTVPFSATMSPVRLHSSNPNLCADIEFQTPPSHLTDPLESSTDYTKLQEEFCLIAQKVHSLLKSAFNS IAIEKEKLKQMVSEQDHSKGHSTQMARLRQSLSQALNQNAELRSRLNRIHSESIICDQVVSVNIIPSPDE AGEQIHVSLPLSQQVANESRLSMSESVSEFFDAQEVLLSASSSENEASDDESYISDVSDNISEDNTSVAD NISRQILNGELTGGAFRNGRRACLPAPCPDTSNINLWNILRNNIGKDLSKVSMPVELNEPLNTLQHLCEE MEYSELLDKASETDDPYERMVLVAAFAVSGYCSTYFRAGSKPFNPVLGETYECIREDKGFRFFSEQVSHH PPISACHCESKNFVFWQDIRWKNKFWGKSMEILPVGTLNVMLPKYGDYYVWNKVTTCIHNILSGRRWIEH YGEVTIRNTKSSVCICKLTFVKVNYWNSNMNEVQGVVIDQEGKAVYRLFGKWHEGLYCGVAPSAKCIWRP GSMPTNYELYYGFTRFAIELNELDPVLKDLLPPTDARFRPDQRFLEEGNLEAAASEKQRVEELQRSRRRY MEENNLEHIPKFFKKVIDANQREAWVSNDTYWELRKDPGFSKVDSPVLW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001201480 |
ORF Size | 2877 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001201480.2 |
RefSeq Size | 7315 bp |
RefSeq ORF | 2880 bp |
Locus ID | 114880 |
UniProt ID | Q9BZF3 |
Cytogenetics | 2q31.2 |
Gene Summary | This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
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