TNIP1 (NM_001258456) Human Tagged ORF Clone

CAT#: RG234424

TNIP1 (tGFP-tagged) - Homo sapiens TNFAIP3 interacting protein 1 (TNIP1), transcript variant 10

Product Images

Circular map for RG234424

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TNIP1
• Synonyms: ABIN-1; NAF1; nip40-1; VAN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234424 representing NM_001258456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGAGAGGACCGTACCGGATCTACGACCCTGGGGGCAGCGTGCCCTCAGGAGAGGCATCCGCAG
  CTTTTGAGCGCCTAGTGAAGGAGAATTCCCGGCTGAAGGAAAAAATGCAAGGGATAAAGATGTTAGGGGA
  GCTTTTGGAAGAGTCCCAGATGGAAGCGACCAGGCTCCGGCAGAAGGCAGAGGAGCTAGTGAAGGACAAC
  GAGCTGCTCCCACCACCTTCTCCCTCCTTGGGCTCCTTCGACCCCCTGGCTGAGCTCACAGGAAAGGACT
  CAAATGTCACAGCATCTCCCACAGCCCCTGCATGCCCCAGTGACAAGCCAGCACCAGTCCAGAAGCCTCC
  ATCCAGTGGCACCTCCTCTGAATTTGAAGTGGTCACTCCTGAGGAGCAGAATTCACCAGAGAGCAGCAGC
  CATGCCAATGCGATGGCGCTGGGCCCCCTGCCCCGTGAGGACGGCAACCTGATGCTGCACCTGCAGCGCC
  TGGAGACCACGCTGAGTGTGTGTGCCGAGGAGCCGGACCACGGCCAGCTCTTCACCCACCTGGGCCGCAT
  GGCCCTGGAGTTCAACCGACTGGCATCCAAGGTGCACAAGAATGAGCAGCGCACCTCCATTCTGCAGACC
  CTGTGTGAGCAGCTTCGGAAGGAGAACGAGGCTCTGAAGGCCAAGTTGGATAAGGGCCTGGAACAGCGGG
  ATCAGGCTGCCGAGAGGCTGCGGGAGGAAAATTTGGAGCTCAAGAAGTTGTTGATGAGCAATGGCAACAA
  AGAGGGTGCGTCTGGGCGGCCAGGCTCACCGAAGATGGAAGGGACAGGCAAGAAGGCAGTGGCTGGACAG
  CAGCAGGCTAGTGTGACGGCAGGTAAGGTCCCAGAGGTGGTGGCCTTGGGCGCAGCCGAGAAGAAGGTGA
  AGATGCTGGAGCAGCAGCGCAGTGAGCTGCTGGAAGTGAACAAGCAGTGGGACCAGCATTTCCGGTCCAT
  GAAGCAGCAGTATGAGCAGAAGATCACTGAGCTGCGTCAGAAGCTGGCTGATTTGCAGAAGCAGGTGACT
  GACCTGGAGGCCGAGCGGGAGCAGAAGCAGCGTGACTTTGACCGCAAGCTCCTCCTGGCCAAGTCCAAGA
  TTGAAATGGAGGAGACCGACAAGGAGCAGCTGACAGCAGAGGCCAAGGAGCTGCGCCAAAAGGTCAAGTA
  CCTGCAGGATCAGCTGAGCCCACTCACCCGACAGCGTGAGTACCAGGAAAAGGAGATCCAGCGGCTCAAC
  AAGGCCCTGGAGGAAGCACTGAGCATCCAAACCCCGCCATCATCTCCACCAACAGCATTTGGGAGCCCAG
  AAGGAGCAGGGGCCCTCCTAAGGAAACAGGAGCTGGTCACGCAGAATGAGTTGCTGAAACAGCAGGTGAA
  GATCTTCGAGGAGGACTTCCAGAGGGAGCGCAGTGATCGTGAGCGCATGAATGAGGAGAAGGAAGAGCTG
  AAGAAGCAAGTGGAGAAGCTGCAGGCCCAGGTCACCCTGTCAAATGCCCAGCTAAAAGCATTCAAAGATG
  AGGAGAAGGCAAGAGAAGCCCTCAGACAGCAGAAGAGGAAAGCAAAGAGTCTCCAAAAAATGACCGTGAG
  GGGCCTCAGTGAGACCAGATTGTGTCATTTGGCTCCACCTTCATCTTGCAGAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234424 representing NM_001258456
  Red=Cloning site Green=Tags(s)
  
  MEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKMLGELLEESQMEATRLRQKAEELVKDN
  ELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPACPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSS
  HANAMALGPLPREDGNLMLHLQRLETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQT
  LCEQLRKENEALKAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQ
  QQASVTAGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVT
  DLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLN
  KALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEEL
  KKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKSLQKMTVRGLSETRLCHLAPPSSCRAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258456
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258456.1, NP_001245385.1
• RefSeq Size: 2428 bp
• RefSeq ORF: 1671 bp
• Locus ID: 10318
• UniProt ID: Q15025
• Cytogenetics: 5q33.1
• Gene Summary: This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]