DATF1 (DIDO1) (NM_001193370) Human Tagged ORF Clone
CAT#: RG233206
- TrueORF®
DIDO1 (tGFP-tagged) - Homo sapiens death inducer-obliterator 1 (DIDO1), transcript variant 6
ORF Plasmid: DDK
"NM_001193370" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | DATF1 |
Synonyms | BYE1; C20orf158; DATF-1; DATF1; DIDO2; DIDO3; DIO-1; DIO1; dJ885L7.8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233206 representing NM_001193370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACAAAGGCGACCCGAGCAATGAGGAGGCACCTAAGGCCATCAAACCCACCAGCAAAGAGTTCA GGAAAACATGGGGTTTTCGAAGGACCACTATCGCCAAGCGAGAGGGCGCAGGGGACGCGGAGGCTGACCC ACTGGAGCCGCCACCCCCACAGCAGCAGCTGGGCCTGTCCCTGCGGCGCAGTGGGAGGCAGCCCAAGCGC ACTGAGCGCGTGGAGCAGTTCCTGACCATTGCGCGGCGCCGCGGCAGGAGGAGCATGCCTGTCTCCCTGG AGGATTCTGGTGAGCCCACGTCCTGCCCCGCCACAGACGCCGAGACAGCCTCCGAGGGCAGCGTGGAAAG CGCTTCTGAGACCAGAAGCGGCCCCCAGTCTGCTTCCACAGCTGTGAAGGAACGACCAGCCTCTTCTGAA AAGGTGAAAGGAGGGGATGACCACGATGACACCTCCGATAGTGACAGCGATGGCCTGACCTTGAAAGAGC TTCAGAATCGCCTTCGCAGGAAGCGGGAACAGGAGCCCACTGAGAGGCCCCTGAAAGGGATCCAGAGTCG CCTGCGGAAGAAGCGCCGGGAGGAGGGTCCCGCCGAGACTGTGGGCTCCGAGGCCAGTGACACTGTGGAG GGCGTCCTGCCCAGTAAGCAGGAGCCCGAGAACGATCAGGGGGTTGTGTCCCAGGCTGGGAAAGATGACA GAGAGAGTAAGTTGGAGGGAAAGGCGGCTCAGGACATCAAAGATGAGGAGCCTGGAGACTTGGGCCGACC GAAGCCTGAATGTGAGGGTTACGACCCCAACGCCCTGTATTGCATTTGCCGCCAGCCTCACAACAACAGG TTTATGATTTGCTGTGACCGCTGTGAAGAATGGTTTCATGGCGATTGTGTGGGCATTTCTGAGGCTCGAG GGAGGCTTTTGGAAAGGAATGGGGAAGACTATATCTGCCCAAACTGCACCATTCTGCAAGTGCAGGATGA GACTCATTCAGAAACGGCAGATCAGCAGGAAGCTAAATGGAGACCTGGAGATGCTGATGGCACCGATTGT ACAAGTATAGGAACAATAGAGCAGAAGTCTAGCGAAGACCAAGGGATAAAGGGTAGAATTGAGAAAGCTG CAAATCCAAGTGGCAAGAAGAAACTCAAGATCTTCCAGCCTGTGATAGAGGCGCCTGGTGCCTCAAAATG TATTGGCCCCGGGTGCTGTCACGTGGCGCAGCCCGACTCGGTGTACTGCAGTAATGACTGTATCCTCAAA CACGCCGCAGCGACAATGAAGTTTCTAAGCTCAGGTAAAGAACAGAAGCCAAAGCCTAAAGAAAAGATGA AGATGAAGCCAGAGAAGCCCAGTCTTCCGAAATGCGGTGCTCAGGCAGGTATTAAAATCTCTTCTGTGCA CAAGAGACCAGCTCCAGAAAAAAAAGAGACCACAGTGAAGAAGGCAGTGGTGGTCCCTGCGCGGAGTGAA GCACTCGGGAAGGAAGCAGCTTGTGAGAGCAGCACGCCGTCGTGGGCGAGCGATCACAATTACAATGCAG TAAAGCCAGAAAAGACTGCTGCTCCCTCGCCGTCACTGTTGTATAAATCCACGAAGGAAGACAGGAGGTC CGAGGAGAAAGCGGCAGCCATGGCAGCCTCAAAGAAAACAGCCCCTCCAGGCTCCGCGGTGGGCAAGCAG CCTGCACCTAGAAACCTCGTGCCAAAGAAGTCTTCTTTTGCTAATGTGGCAGCAGCCACACCAGCCATTA AAAAGCCACCCTCAGGTTTCAAGGGCACCATCCCCAAGAGGCCATGGCTCTCCGCTACCCCATCGAGTGG TGCTTCAGCTGCCAGGCAGGCCGGACCGGCACCTGCAGCGGCAACGGCTGCCTCCAAGAAGTTCCCTGGC TCCGCTGCTTTGGTGGGAGCCGTAAGGAAGCCAGTGGTACCTTCTGTTCCAATGGCCTCGCCAGCCCCAG GACGCCTTGGGGCTATGAGTGCTGCACCATCGCAGCCAAATTCACAAATTCGGCAAAATATCAGACGCTC CTTAAAAGAGATTTTGTGGAAAAGAGTCAATGACAGCGATGACTTAATCATGACAGAAAACGAAGTAGGA AAAATTGCCCTCCATATTGAGAAGGAGATGTTTAACTTGTTTCAAGTTACAGATAATCGCTACAAGAGTA AATATCGCAGCATCATGTTCAACCTGAAGGACCCTAAAAATCAGGGACTCTTCCATCGTGTTCTGCGTGA GGAAATCTCTTTGGCGAAACTTGTGAGACTGAAGCCAGAAGAACTTGTATCTAAAGAGCTTTCCACGTGG AAAGAGAGGCCAGCGAGATCTGTGATGGAGTCCAGAACTAAACTGCACAATGAAAGCAAGAAGACGGCCC CCAGGCAGGAGGCCATCCCCGATCTGGAGGACTCTCCGCCAGTGTCGGATTCAGAGGAACAGCAAGAGTC AGCACGTGCTGTCCCTGAGAAGAGCACAGCGCCGCTTCTCGACGTCTTCAGCAGCATGTTGAAAGACACC ACCAGTCAGCACCGCGCACACCTCTTCGATCTCAACTGTAAAATTTGCACAGGCCAGGTTCCCTCCGCAG AAGATGAGCCAGCTCCGAAAAAACAAAAATTGTCAGCTTCTGTTAAGAAAGAAGACTTAAAATCAAAGCA TGACAGCTCTGCACCTGACCCAGCTCCGGATTCAGCTGATGAGGTGATGCCGGAGGCTGTGCCTGAAGTT GCCTCTGAGCCAGGCCTAGAAAGTGCTTCTCATCCAAATGTGGACAGAACGTATTTCCCTGGGCCTCCAG GAGATGGCCATCCCGAGCCCTCCCCGCTGGAAGACCTGTCCCCCTGCCCAGCCTCCTGTGGGAGCGGGGT GGTCACCACCGTCACAGTGTCCGGCCGGGACCCCAGGACCGCTCCAAGCAGTTCATGCACAGCCGTGGCC TCCGCAGCATCCCGCCCAGACAGCACCCACATGGTGGAAGCCAGACAGGATGTGCCGAAGCCTGTCTTGA CTTCTGTGATGGTGCCCAAGTCCATACTAGCTAAGCCATCCTCATCTCCTGACCCAAGATACCTGTCAGT TCCTCCGTCACCAAATATCAGCACTTCAGAATCACGTTCCCCTCCAGAGGGAGACACGACCCTCTTTTTG TCTCGACTCAGCACCATTTGGAAAGGATTTATTAACATGCAGAGTGTGGCAAAATTTGTCACTAAGGCGT ATCCTGTCTCTGGGTGTTTTGATTACCTCAGTGAGGATTTGCCTGACACAATTCACATTGGTGGGAGGAT CGCACCGAAGACAGTTTGGGATTATGTTGGCAAACTCAAGTCTTCTGTGTCTAAGGAGCTCTGTCTGATC CGCTTCCACCCCGCCACAGAGGAAGAGGAGGTCGCCTATATCTCTCTCTACTCCTATTTCAGCAGCCGTG GCCGCTTTGGTGTTGTAGCTAATAACAACAGGCACGTCAAGGACCTCTACCTGATCCCGCTGAGCGCCCA GGACCCTGTTCCATCCAAACTCTTGCCCTTTGAGGGACCAGGTAAGCGCCGGCTTTCTGGGTGGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233206 representing NM_001193370
Red=Cloning site Green=Tags(s) MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEPPPPQQQLGLSLRRSGRQPKR TERVEQFLTIARRRGRRSMPVSLEDSGEPTSCPATDAETASEGSVESASETRSGPQSASTAVKERPASSE KVKGGDDHDDTSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAETVGSEASDTVE GVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEEPGDLGRPKPECEGYDPNALYCICRQPHNNR FMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDC TSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILK HAAATMKFLSSGKEQKPKPKEKMKMKPEKPSLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSE ALGKEAACESSTPSWASDHNYNAVKPEKTAAPSPSLLYKSTKEDRRSEEKAAAMAASKKTAPPGSAVGKQ PAPRNLVPKKSSFANVAAATPAIKKPPSGFKGTIPKRPWLSATPSSGASAARQAGPAPAAATAASKKFPG SAALVGAVRKPVVPSVPMASPAPGRLGAMSAAPSQPNSQIRQNIRRSLKEILWKRVNDSDDLIMTENEVG KIALHIEKEMFNLFQVTDNRYKSKYRSIMFNLKDPKNQGLFHRVLREEISLAKLVRLKPEELVSKELSTW KERPARSVMESRTKLHNESKKTAPRQEAIPDLEDSPPVSDSEEQQESARAVPEKSTAPLLDVFSSMLKDT TSQHRAHLFDLNCKICTGQVPSAEDEPAPKKQKLSASVKKEDLKSKHDSSAPDPAPDSADEVMPEAVPEV ASEPGLESASHPNVDRTYFPGPPGDGHPEPSPLEDLSPCPASCGSGVVTTVTVSGRDPRTAPSSSCTAVA SAASRPDSTHMVEARQDVPKPVLTSVMVPKSILAKPSSSPDPRYLSVPPSPNISTSESRSPPEGDTTLFL SRLSTIWKGFINMQSVAKFVTKAYPVSGCFDYLSEDLPDTIHIGGRIAPKTVWDYVGKLKSSVSKELCLI RFHPATEEEEVAYISLYSYFSSRGRFGVVANNNRHVKDLYLIPLSAQDPVPSKLLPFEGPGKRRLSGWR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001193370 |
ORF Size | 3567 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193370.2 |
RefSeq Size | 7613 bp |
RefSeq ORF | 3570 bp |
Locus ID | 11083 |
UniProt ID | Q9BTC0 |
Cytogenetics | 20q13.33 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. [provided by RefSeq, Jul 2008] |
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