SATB1 (NM_001195470) Human Tagged ORF Clone

CAT#: RG231415

SATB1 (tGFP-tagged) - Human SATB homeobox 1 (SATB1), transcript variant 3

Product Images

Circular map for RG231415

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SATB1
• Synonyms: DEFDA; KTZSL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231415 representing NM_001195470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGTGATCCGA
  AGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTAGGAAGAGGAAGGCTTGGGAG
  TACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCGGGCCATCTGATGAAAACCAACCTTAGGAAA
  GGAACCATGCTGCCAGTTTTCTGTGTGGTGGAACATTATGAAAACGCCATTGAATATGATTGCAAGGAGG
  AGCATGCAGAATTTGTGCTGGTGAGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTC
  TCTAGGTTATTCACATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTT
  CCACTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTGTATCATGTGG
  TCACATTGAAAATTCAGTTACACAGTTGCCCCAAACTAGAAGACTTGCCTCCCGAACAATGGTCGCACAC
  CACAGTGAGGAATGCTCTGAAGGACTTACTGAAAGATATGAATCAGAGTTCATTGGCCAAGGAGTGCCCC
  CTTTCACAGAGTATGATTTCTTCCATTGTGAACAGTACTTACTATGCAAATGTCTCAGCAGCAAAATGTC
  AAGAATTTGGAAGGTGGTACAAACATTTCAAGAAGACAAAAGATATGATGGTTGAAATGGATAGTCTTTC
  TGAGCTATCCCAGCAAGGCGCCAATCATGTCAATTTTGGCCAGCAACCAGTTCCAGGGAACACAGCCGAG
  CAGCCTCCATCCCCTGCGCAGCTCTCCCATGGCAGCCAGCCCTCTGTCCGGACACCTCTTCCAAACCTGC
  ACCCTGGGCTCGTATCAACACCTATCAGTCCTCAATTGGTCAACCAGCAGCTGGTGATGGCTCAGCTGCT
  GAACCAGCAGTATGCAGTGAATAGACTTTTAGCCCAGCAGTCCTTAAACCAACAATACTTGAACCACCCT
  CCCCCTGTCAGTAGATCTATGAATAAGCCTTTGGAGCAACAGGTTTCGACCAACACAGAGGTGTCTTCCG
  AAATCTACCAGTGGGTACGCGATGAACTGAAACGAGCAGGAATCTCCCAGGCGGTATTTGCACGTGTGGC
  TTTTAACAGAACTCAGGGCTTGCTTTCAGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCCAG
  TCTTTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTGCAGTTACCGGAAGCTGAAAGAGACCGAATAT
  ACCAGGACGAAAGGGAAAGGAGCTTGAATGCTGCCTCGGCCATGGGTCCTGCCCCCCTCATCAGCACACC
  ACCCAGCCGTCCTCCCCAGGTGAAAACAGCTACTATTGCCACTGAAAGGAATGGGAAACCAGAGAACAAT
  ACCATGAACATTAATGCTTCCATTTATGATGAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCTCAAG
  CACTGTTTGCAAAGGTTGCAGCAACCAAAAGCCAGGGATGGTTGTGCGAGCTGTTACGCTGGAAAGAAGA
  TCCTTCTCCAGAAAACAGAACCCTGTGGGAGAACCTCTCCATGATCCGAAGGTTCCTCAGTCTTCCTCAG
  CCAGAACGTGATGCCATTTATGAACAGGAGAGCAACGCGGTGCATCACCATGGCGACAGGCCGCCCCACA
  TTATCCATGTTCCAGCAGAGCAGATTCAGAGCCCCTCTCCCACCACCCTTGGGAAAGGAGAGTCTAGAGG
  CGTTTTCTTACCAGGCCTGCCGACCCCTGCACCATGGCTCGGTGCTGCTCCTCAGCAACAGCAGCAGCAA
  CAGCAACAGCAGCAGCAGCAGCAGCAGGCACCGCCGCCTCCACAGCCACAGCAGCAGCCACAGACAGGCC
  CTCGGCTCCCCCCACGGCAACCCACGGTGGCCTCTCCAGCAGAGTCAGATGAGGAAAACCGACAGAAGAC
  CCGGCCACGAACAAAAATTTCAGTGGAAGCCTTGGGAATCCTCCAGAGTTTCATACAAGACGTGGGCCTG
  TACCCTGACGAAGAGGCCATCCAGACTCTGTCTGCCCAGCTCGACCTTCCCAAGTACACCATCATCAAGT
  TCTTTCAGAACCAGCGGTACTATCTCAAGCACCACGGCAAACTGAAGGACAATTCCGGTTTAGAGGTCGA
  TGTGGCAGAATATAAAGAAGAGGAGCTGCTGAAGGATTTGGAAGAGAGTGTCCAAGATAAAAATACTAAC
  ACCCTTTTTTCAGTGAAACTAGAAGAAGAGCTGTCAGTGGAAGGAAACACAGACATTAATACTGATTTGA
  AAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231415 representing NM_001195470
  Red=Cloning site Green=Tags(s)
  
  MDHLNEATQGKEHSEMSNNVSDPKGPPAKIARLEQNGSPLGRGRLGSTGAKMQGVPLKHSGHLMKTNLRK
  GTMLPVFCVVEHYENAIEYDCKEEHAEFVLVRKDMLFNQLIEMALLSLGYSHSSAAQAKGLIQVGKWNPV
  PLSYVTDAPDATVADMLQDVYHVVTLKIQLHSCPKLEDLPPEQWSHTTVRNALKDLLKDMNQSSLAKECP
  LSQSMISSIVNSTYYANVSAAKCQEFGRWYKHFKKTKDMMVEMDSLSELSQQGANHVNFGQQPVPGNTAE
  QPPSPAQLSHGSQPSVRTPLPNLHPGLVSTPISPQLVNQQLVMAQLLNQQYAVNRLLAQQSLNQQYLNHP
  PPVSRSMNKPLEQQVSTNTEVSSEIYQWVRDELKRAGISQAVFARVAFNRTQGLLSEILRKEEDPKTASQ
  SLLVNLRAMQNFLQLPEAERDRIYQDERERSLNAASAMGPAPLISTPPSRPPQVKTATIATERNGKPENN
  TMNINASIYDEIQQEMKRAKVSQALFAKVAATKSQGWLCELLRWKEDPSPENRTLWENLSMIRRFLSLPQ
  PERDAIYEQESNAVHHHGDRPPHIIHVPAEQIQSPSPTTLGKGESRGVFLPGLPTPAPWLGAAPQQQQQQ
  QQQQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQSFIQDVGL
  YPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYKEEELLKDLEESVQDKNTN
  TLFSVKLEEELSVEGNTDINTDLKD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195470
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195470.3
• RefSeq Size: 5652 bp
• RefSeq ORF: 2388 bp
• Locus ID: 6304
• UniProt ID: Q01826
• Cytogenetics: 3p24.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. [provided by RefSeq, Apr 2016]