APOBEC3D (NM_152426) Human Tagged ORF Clone

CAT#: RG229185

APOBEC3D (tGFP-tagged) - Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D (APOBEC3D)

Product Images

Circular map for RG229185

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: APOBEC3D
• Synonyms: A3D; A3DE; APOBEC3DE; APOBEC3E; ARP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229185 representing NM_152426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCACAGATCAGAAATCCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACG
  AACCCATCCTCTATGGTCGGAGCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAA
  TCTCCTTTGGGACACAGGGGTCTTTCGAGGCCCGGTACTACCCAAACGTCAGTCGAATCACAGGCAGGAG
  GTGTATTTCCGGTTTGAGAACCACGCAGAAATGTGCTTCTTATCTTGGTTCTGTGGCAACCGACTGCCTG
  CTAACAGGCGCTTCCAGATCACCTGGTTTGTATCATGGAACCCCTGCCTGCCCTGTGTGGTGAAGGTGAC
  CAAATTCTTGGCTGAGCACCCCAATGTCACCCTGACCATCTCTGCCGCCCGCCTCTACTACTACCGGGAT
  AGAGATTGGCGGTGGGTGCTCCTCAGGCTGCATAAGGCAGGGGCCCGTGTGAAGATCATGGACTATGAAG
  ACTTTGCATACTGCTGGGAAAACTTTGTGTGCAATGAAGGTCAGCCATTCATGCCTTGGTACAAATTCGA
  TGACAATTATGCATCCCTGCACCGCACGCTAAAGGAGATTCTCAGAAACCCGATGGAGGCAATGTACCCA
  CACATATTCTACTTCCACTTTAAAAACCTACTGAAAGCCTGTGGTCGGAACGAAAGCTGGCTGTGCTTCA
  CCATGGAAGTTACAAAGCACCACTCAGCTGTCTTCCGGAAGAGGGGCGTCTTCCGAAACCAGGTGGATCC
  TGAGACCCATTGTCATGCAGAAAGGTGCTTCCTCTCTTGGTTCTGTGACGACATACTGTCTCCTAACACA
  AACTACGAGGTCACCTGGTACACATCTTGGAGCCCTTGCCCAGAGTGTGCAGGGGAGGTGGCCGAGTTCC
  TGGCCAGGCACAGCAACGTGAATCTCACCATCTTCACCGCCCGCCTCTGCTACTTCTGGGATACAGATTA
  CCAGGAGGGGCTCTGCAGCCTGAGTCAGGAAGGGGCCTCCGTGAAGATCATGGGCTACAAAGATTTTGTA
  TCTTGTTGGAAAAACTTTGTGTACAGTGATGATGAGCCATTCAAGCCTTGGAAGGGACTACAAACCAACT
  TTCGACTTCTGAAAAGAAGGCTACGGGAGATTCTCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229185 representing NM_152426
  Red=Cloning site Green=Tags(s)
  
  MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGPVLPKRQSNHRQE
  VYFRFENHAEMCFLSWFCGNRLPANRRFQITWFVSWNPCLPCVVKVTKFLAEHPNVTLTISAARLYYYRD
  RDWRWVLLRLHKAGARVKIMDYEDFAYCWENFVCNEGQPFMPWYKFDDNYASLHRTLKEILRNPMEAMYP
  HIFYFHFKNLLKACGRNESWLCFTMEVTKHHSAVFRKRGVFRNQVDPETHCHAERCFLSWFCDDILSPNT
  NYEVTWYTSWSPCPECAGEVAEFLARHSNVNLTIFTARLCYFWDTDYQEGLCSLSQEGASVKIMGYKDFV
  SCWKNFVYSDDEPFKPWKGLQTNFRLLKRRLREILQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152426
• ORF Size: 1158 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152426.4
• RefSeq Size: 2519 bp
• RefSeq ORF: 1161 bp
• Locus ID: 140564
• Cytogenetics: 22q13.1
• Gene Summary: This gene is a member of the cytidine deaminase gene family. It is one of a group of related genes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1 and inhibit retroviruses, such as HIV, by deaminating cytosine residues in nascent retroviral cDNA. [provided by RefSeq, Jul 2008]