NSP3 (SH2D3C) (NM_001142533) Human Tagged ORF Clone

CAT#: RG227430

SH2D3C (tGFP-tagged) - Human SH2 domain containing 3C (SH2D3C), transcript variant 5

Product Images

Circular map for RG227430

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NSP3
• Synonyms: CHAT; NSP3; PRO34088; SHEP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227430 representing NM_001142533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGCGGTGCAGCCTGTGGAGTGCCCTGTCGGCCGCCGCCTGCTGCTTCTACCGCGGCTCCTTCG
  TGCAGGTGCAGTTCTCCAAGGAGAAGTACATCCTGGACTCATCGCCAGAGAAACTCCACAAGGAATTGGA
  GGAGGAGCTCAAACTCAGCAGCACGGATCTCCGCAGCCATGCCTGGTACCATGGCCGCATCCCCCGAGAG
  GTCTCGGAGACCTTGGTACAACGCAACGGCGACTTCCTCATCCGGGACTCACTCACCAGCCTGGGCGACT
  ATGTGCTCACGTGCCGCTGGCGCAACCAGGCCTTGCACTTCAAGATCAACAAGGTGGTGGTGAAGGCAGG
  CGAGAGCTACACACACATCCAGTACCTGTTTGAGCAGGAGAGCTTTGACCACGTGCCCGCCCTCGTGCGC
  TATCATGTGGGCAGCCGCAAGGCTGTGTCAGAGCAGAGTGGTGCCATCATCTACTGCCCGGTGAACCGCA
  CCTTCCCACTGCGCTACCTCGAGGCCAGCTATGGCCTGGGACAGGGGAGTAGCAAGCCTGCTAGCCCCGT
  CAGCCCCTCAGGCCCCAAGGGCAGCCACATGAAGCGGCGCAGCGTCACCATGACCGATGGGCTCACTGCT
  GACAAGGTCACCCGCAGCGATGGCTGCCCCACCAGTACGTCGCTGCCCCGCCCTCGGGACTCCATCCGCA
  GCTGTGCCCTCAGCATGGACCAGATCCCAGACCTGCACTCACCCATGTCGCCCATCTCCGAGAGCCCTAG
  CTCCCCTGCCTACAGCACTGTAACCCGTGTCCATGCCGCCCCTGCAGCCCCTTCTGCCACAGCATTGCCT
  GCCTCCCCTGTCGCCCGCCGTTCCAGTGAGCCCCAGCTGTGTCCCGGAAGTGCCCCAAAGACCCATGGGG
  AGTCAGACAAGGGCCCCCACACCAGCCCCTCCCACACCCTTGGCAAGGCCTCCCCGTCACCATCACTCAG
  CAGCTACAGTGACCCGGACTCTGGCCACTACTGCCAGCTCCAGCCTCCCGTGCGTGGCAGCCGAGAGTGG
  GCAGCGACTGAGACCTCCAGCCAGCAGGCCAGGAGCTATGGGGAGAGGCTAAAGGAACTGTCAGAAAATG
  GGGCCCCTGAAGGGGACTGGGGCAAGACCTTCACAGTCCCCATCGTGGAAGTCACTTCTTCCTTCAACCC
  GGCCACCTTCCAGTCACTACTGATCCCCAGGGATAACCGGCCACTGGAGGTGGGCCTTCTGCGCAAGGTC
  AAGGAGCTGCTGGCAGAAGTGGATGCCCGGACGCTGGCCCGGCATGTCACCAAGGTGGACTGCCTGGTTG
  CTAGGATACTGGGCGTTACCAAGGAGATGCAGACCCTAATGGGAGTCCGCTGGGGCATGGAACTGCTCAC
  CCTCCCCCATGGCCGGCAGCTACGCCTAGACCTGCTGGAAAGGTTCCACACCATGTCCATCATGCTGGCC
  GTGGACATCCTGGGCTGCACCGGCTCTGCGGAGGAGCGGGCAGCGCTGCTGCACAAGACCATTCAGCTGG
  CGGCCGAGCTGCGGGGGACTATGGGCAACATGTTCAGCTTCGCGGCGGTCATGGGTGCCCTGGACATGGC
  CCAGATTTCTCGGCTGGAGCAGACATGGGTGACCCTGCGGCAGCGACACACAGAGGGTGCCATCCTGTAC
  GAGAAGAAGCTCAAGCCTTTTCTCAAGAGCCTCAACGAGGGCAAAGAAGGCCCGCCGCTGAGCAACACCA
  CGTTTCCTCATGTGCTGCCCCTCATCACCCTGCTGGAGTGTGACTCGGCCCCACCAGAGGGCCCTGAGCC
  CTGGGGCAGCACGGAGCACGGCGTGGAGGTGGTGCTGGCTCACCTGGAGGCCGCCCGCACAGTGGCACAC
  CACGGAGGCCTGTACCACACCAATGCTGAAGTCAAGCTGCAGGGGTTCCAGGCCCGGCCGGAGCTCCTGG
  AGGTGTTCAGCACGGAGTTCCAGATGCGCCTTCTCTGGGGCAGTCAGGGTGCCAGCAGCAGCCAGGCCCG
  GCGCTATGAGAAGTTCGACAAGGTCCTCACTGCCCTGTCCCACAAGCTGGAACCTGCTGTCCGCTCCAGC
  GAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227430 representing NM_001142533
  Red=Cloning site Green=Tags(s)
  
  MTERCSLWSALSAAACCFYRGSFVQVQFSKEKYILDSSPEKLHKELEEELKLSSTDLRSHAWYHGRIPRE
  VSETLVQRNGDFLIRDSLTSLGDYVLTCRWRNQALHFKINKVVVKAGESYTHIQYLFEQESFDHVPALVR
  YHVGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGLGQGSSKPASPVSPSGPKGSHMKRRSVTMTDGLTA
  DKVTRSDGCPTSTSLPRPRDSIRSCALSMDQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPSATALP
  ASPVARRSSEPQLCPGSAPKTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPDSGHYCQLQPPVRGSREW
  AATETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRKV
  KELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLA
  VDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILY
  EKKLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLEAARTVAH
  HGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVRSS
  EL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142533
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142533.1, NP_001136005.1
• RefSeq Size: 2694 bp
• RefSeq ORF: 2109 bp
• Locus ID: 10044
• UniProt ID: Q8N5H7
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein contains a putative Src homology 2 (SH2) domain and guanine nucleotide exchange factor-like domain which allows this signaling protein to form a complex with scaffolding protein Crk-associated substrate. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]