POTEC (NM_001137671) Human Tagged ORF Clone

CAT#: RG227037

POTEC (tGFP-tagged) - Human POTE ankyrin domain family, member C (POTEC)

Product Images

Circular map for RG227037

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POTEC
• Synonyms: A26B2; CT104.6; POTE-18; POTE18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227037 representing NM_001137671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTGAGGTTTGTTCAATGCCCGCTGCCTCTGCTGTGAAGAAGCCATTCGATCTCAGGAGCAAGA
  TGGGCAAGTGGTTTCACCACCGCTTCCCCTGCTGCAAGGGGAGCGGCAAGAGCAACATGGGCACTTCTGG
  AGACCACGACGACTCCTTTATGAAGATGCTCAGGAGCAAGATGGGCAAGTGTTGCCACCACTGCTTCCCC
  TGCTGCAGGGGGAGCGGCACGAGCAACGTGGGCACTTCTGGAGACCATGACAACTCCTTTATGAAGACGC
  TCAGGAGCAAGATGGGCAAGTGGTGCTGTCACTGCTTCCCCTGCTGCAGGGGGAGCGGCAAGAGCAACGT
  GGGCGCTTGGGGAGACTACGACGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATCTG
  GACAAGCTCCACAGAGCTGCCTGGTGGGGTAAGGTCCCCAGAAAGGATCTCATCGTCATGCTCAGGGACA
  CGGACATGAACAAGAGGGACAAGCAAAAGAGGACTGCTCTACATTTGGCCTCTGCCAATGGAAATTCAGA
  AGTAGTACAACTCCTGCTGGACAGACGATGTCAACTTAATGTCCTTGACAACAAAAAAAGGACAGCTCTG
  ATAAAGGCCGTACAATGCCAGGAAGATGAATGTGTGTTAATGTTGCTGGAACATGGCGCTGATCAAAATA
  TTCCAGATGAGTATGGAAATACCACTCTACACTATGCTGTCCACAATGAAGATAAATTAATGGCCAAAGC
  ACTGCTCTTATATGGTGCTGATATTGAATCAAAAAACAAGTGTGGCCTCACACCACTTTTGCTTGGCGTA
  CATGAACAAAAACAGCAAGTGGTGAAATTTTTAATCAAGAAAAAAGCTAATTTAAATGCACTTGATAGAT
  ATGGAAGAACTGCCCTCATACTTGCTGTATGTTGTGGATCAGCAAGTATAGTCAATCTTCTCCTTGAGCA
  AAATGTTGATGTATCTTCTCAAGATCTATCTGGACAGACGGCCAGAGAGTATGCTGTTTCTAGTCATCAT
  CATGTAATTTGTGAATTACTTTCTGACTATAAAGAAAAACAGATGCTAAAAATCTCTTCTGAAAACAGCA
  ATCCAGAACAAGACTTAAAGCTAACATCAGAGGAAGAGTCACAAAGGCTTAAAGTCAGTGAAAATAGCCA
  GCCAGAGAAAATGTCTCAAGAACCAGAAATAAATAAGGACTGTGATAGAGAGGTTGAAGAAGAAATAAAG
  AAGCATGGAAGTAATCCTGTGGGATTACCAGAAAACCTGACTAATGGTGCCAGTGCTGGCAATGGTGATG
  ATGGATTAATTCCACAAAGGAGGAGCAGAAAACCTGAAAATCAGCAATTTCCTGACACTGAGAATGAAGA
  GTATCACAGTGACGAACAAAATGATACCCGGAAACAACTTTCTGAAGAACAGAACACTGGAATATCACAA
  GATGAGATTCTGACTAATAAACAAAAGCAGATAGAAGTGGCTGAAAAGAAAATGAATTCTGAGCTTTCTC
  TTAGTCATAAGAAAGAAGAAGATCTCTTGCGTGAAAACAGCATGTTGCAGGAAGAAATTGCCATGCTAAT
  TTCTGGAGACTGGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227037 representing NM_001137671
  Red=Cloning site Green=Tags(s)
  
  MVTEVCSMPAASAVKKPFDLRSKMGKWFHHRFPCCKGSGKSNMGTSGDHDDSFMKMLRSKMGKCCHHCFP
  CCRGSGTSNVGTSGDHDNSFMKTLRSKMGKWCCHCFPCCRGSGKSNVGAWGDYDDSAFMEPRYHVRREDL
  DKLHRAAWWGKVPRKDLIVMLRDTDMNKRDKQKRTALHLASANGNSEVVQLLLDRRCQLNVLDNKKRTAL
  IKAVQCQEDECVLMLLEHGADQNIPDEYGNTTLHYAVHNEDKLMAKALLLYGADIESKNKCGLTPLLLGV
  HEQKQQVVKFLIKKKANLNALDRYGRTALILAVCCGSASIVNLLLEQNVDVSSQDLSGQTAREYAVSSHH
  HVICELLSDYKEKQMLKISSENSNPEQDLKLTSEEESQRLKVSENSQPEKMSQEPEINKDCDREVEEEIK
  KHGSNPVGLPENLTNGASAGNGDDGLIPQRRSRKPENQQFPDTENEEYHSDEQNDTRKQLSEEQNTGISQ
  DEILTNKQKQIEVAEKKMNSELSLSHKKEEDLLRENSMLQEEIAMLISGDWN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001137671
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001137671.1, NP_001131143.1
• RefSeq Size: 2243 bp
• RefSeq ORF: 1629 bp
• Locus ID: 388468
• UniProt ID: B2RU33
• Cytogenetics: 18p11.21