RBM27 (NM_018989) Human Tagged ORF Clone

CAT#: RG226315

RBM27 (tGFP-tagged) - Human RNA binding motif protein 27 (RBM27)

Product Images

Circular map for RG226315

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBM27
• Synonyms: ARRS1; Psc1; ZC3H18; ZC3H20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226315 representing NM_018989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCATAGAGGATGTGGATGCCCTCAAGTCCTGGCTGGCCAAGTTACTGGAGCCGATATGTGATGCTG
  ATCCTTCAGCCTTAGCCAACTATGTTGTAGCACTGGTCAAGAAGGACAAACCTGAGAAAGAATTAAAAGC
  CTTTTGTGCTGATCAACTTGATGTCTTTTTACAAAAAGAAACTTCAGGTTTTGTGGACAAACTATTTGAA
  AGTCTCTATACTAAGAACTACCTTCCACTTTTGGAACCAGTAAAGCCTGAGCCAAAACCACTAGTCCAAG
  AAAAAGAAGAAATTAAAGAAGAGGTATTTCAGGAGCCAGCAGAGGAAGAACGAGATGGCAGAAAAAAGAA
  ATATCCTAGTCCCCAGAAGACTCGTTCAGAATCTAGTGAACGAAGGACACGTGAGAAAAAAAGAGAAGAC
  GGGAAATGGAGAGACTATGACCGGTACTATGAGCGGAATGAATTGTACCGTGAGAAGTATGACTGGAGAA
  GAGGCAGGAGTAAGAGTCGGAGTAAGAGTCGAGGCCTGAGTCGCAGTAGAAGCCGAAGTAGGGGGCGCAG
  CAAAGACCGGGATCCAAATAGGAATGTTGAGCACAGGGAAAGATCGAAGTTTAAGAGTGAAAGGAATGAC
  CTGGAGAGTTCCTATGTGCCTGTGTCTGCACCACCTCCAAACTCTTCTGAGCAGTATTCCTCTGGGGCAC
  AGTCTATTCCCAGCACTGTTACTGTGATCGCACCTGCTCACCACTCTGAAAACACAACTGAGAGTTGGTC
  TAATTACTATAACAATCATAGCTCTTCCAATTCTTTTGGTCGAAACCTACCACCAAAGAGGCGATGCAGA
  GATTATGATGAAAGAGGATTTTGTGTACTTGGTGACCTTTGTCAGTTTGATCATGGAAATGATCCCCTAG
  TTGTTGATGAAGTTGCTCTGCCAAGTATGATTCCTTTCCCACCCCCTCCTCCTGGGCTTCCTCCTCCACC
  ACCTCCTGGAATGTTAATGCCTCCAATGCCAGGTCCAGGCCCAGGCCCGGGCCCAGGTCCAGGCCCAGGC
  CCGGGCCCAGGTCCAGGTCCTGGCCATAGTATGAGACTTCCTGTTCCCCAAGGACATGGTCAGCCTCCAC
  CATCCGTTGTGCTTCCCATACCAAGACCACCTATAACACAATCAAGCTTGATAAACAGCCGTGACCAGCC
  TGGGACAAGTGCAGTGCCCAATCTTGCATCAGTGGGAACAAGACTACCTCCTCCTTTACCCCAGAACCTC
  CTTTACACAGTATCAGAACGACAGCCCATGTACTCTCGTGAACATGGTGCTGCTGCATCTGAGCGACTTC
  AGTTGGGGACACCGCCTCCTCTGTTGGCAGCTCGTTTGGTGCCACCTCGAAACCTCATGGGATCCTCCAT
  TGGATACCATACCTCAGTCTCCAGCCCTACCCCTCTGGTTCCAGATACATATGAACCAGATGGTTACAAC
  CCAGAAGCTCCTAGTATTACTAGTTCTGGTAGATCTCAGTACAGACAGTTCTTTTCAAGAACTCAGACAC
  AGCGTCCCAATCTGATTGGCCTAACATCTGGAGATATGGATGTAAATCCAAGAGCTGCTAACATTGTGAT
  CCAGACTGAACCACCAGTTCCTGTTTCGATTAATAGCAACATAACCAGAGTAGTTCTTGAACCAGATAGT
  CGAAAAAGAGCTATGAGTGGTTTGGAAGGGCCACTCACAAAGAAACCTTGGCTGGGAAAGCAAGGAAATA
  ACAATCAAAATAAACCAGGGTTCTTACGAAAGAATCAGTATACAAACACCAAATTAGAAGTCAAGAAAAT
  CCCTCAGGAATTGAACAACATTACCAAGCTCAATGAACACTTCAGCAAATTTGGAACTATTGTTAATATC
  CAGGTTGCTTTTAAGGGTGACCCAGAAGCAGCCCTAATCCAATATCTTACCAATGAGGAGGCCAGGAAAG
  CCATTTCTAGCACAGAAGCAGTTCTAAACAACCGATTCATTCGAGTCTTGTGGCATAGGGAAAATAATGA
  GCAACCGACACTACAGTCCTCAGCACAGCTGCTCCTGCAACAACAGCAAACACTTAGTCACCTCTCACAG
  CAGCACCATCACCTGCCACAGCATCTACATCAGCAGCAGGTGCTAGTGGCCCAGTCTGCTCCTTCAACAG
  TGCACGGAGGTATCCAGAAGATGATGAGCAAACCACAGACATCAGGTGCATATGTTCTTAACAAAGTTCC
  TGTTAAACATCGTCTTGGACATGCAGGTGGTAACCAGAGTGATGCATCACATTTGTTGAATCAGTCTGGT
  GGTGCTGGAGAAGATTGCCAGATATTTTCAACTCCAGGCCATCCAAAAATGATTTACAGCTCCTCAAACT
  TAAAGACACCTTCAAAGCTCTGTTCAGGGTCTAAATCTCATGATGTTCAAGAAGTGCTTAAAAAAAAACA
  GGAAGCAATGAAGTTACAACAAGATATGAGGAAAAAAAGACAGGAAGTGTTAGAAAAGCAAATAGAATGC
  CAAAAGATGTTAATATCCAAGTTAGAAAAAAACAAAAACATGAAACCAGAAGAAAGAGCAAATATAATGA
  AGACTTTGAAAGAGCTTGGAGAGAAGATCTCACAATTAAAAGATGAATTAAAAACATCTTCTGCAGTCTC
  CACACCATCTAAAGTGAAGACAAAAACGGAGGCCCAGAAGGAGTTATTAGATACTGAACTGGACCTCCAC
  AAGAGGCTGTCCTCAGGAGAAGACACCACAGAATTACGGAAAAAACTCAGTCAGTTACAGGTTGAGGCTG
  CACGGTTAGGTATTTTACCTGTGGGTCGAGGAAAGACCATGTCCTCTCAAGGTCGAGGAAGAGGCCGAGG
  GCGTGGAGGAAGAGGAAGGGGCTCACTAAATCACATGGTGGTGGACCATCGTCCCAAAGCACTAACAGTT
  GGAGGATTCATTGAGGAAGAAAAAGAAGACTTGCTTCAGCATTTCTCAACCGCAAACCAAGGGCCAAAAT
  TTAAAGACCGTCGGCTACAGATATCATGGCACAAGCCCAAGGTACCATCTATATCCACTGAGACTGAAGA
  AGAAGAAGTCAAGGAGGAGGAAACAGAAACCTCAGATTTGTTTTTGCCTGATGATGACGATGAAGATGAA
  GATGAATATGAGTCTCGCTCATGGCGAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226315 representing NM_018989
  Red=Cloning site Green=Tags(s)
  
  MLIEDVDALKSWLAKLLEPICDADPSALANYVVALVKKDKPEKELKAFCADQLDVFLQKETSGFVDKLFE
  SLYTKNYLPLLEPVKPEPKPLVQEKEEIKEEVFQEPAEEERDGRKKKYPSPQKTRSESSERRTREKKRED
  GKWRDYDRYYERNELYREKYDWRRGRSKSRSKSRGLSRSRSRSRGRSKDRDPNRNVEHRERSKFKSERND
  LESSYVPVSAPPPNSSEQYSSGAQSIPSTVTVIAPAHHSENTTESWSNYYNNHSSSNSFGRNLPPKRRCR
  DYDERGFCVLGDLCQFDHGNDPLVVDEVALPSMIPFPPPPPGLPPPPPPGMLMPPMPGPGPGPGPGPGPG
  PGPGPGPGHSMRLPVPQGHGQPPPSVVLPIPRPPITQSSLINSRDQPGTSAVPNLASVGTRLPPPLPQNL
  LYTVSERQPMYSREHGAAASERLQLGTPPPLLAARLVPPRNLMGSSIGYHTSVSSPTPLVPDTYEPDGYN
  PEAPSITSSGRSQYRQFFSRTQTQRPNLIGLTSGDMDVNPRAANIVIQTEPPVPVSINSNITRVVLEPDS
  RKRAMSGLEGPLTKKPWLGKQGNNNQNKPGFLRKNQYTNTKLEVKKIPQELNNITKLNEHFSKFGTIVNI
  QVAFKGDPEAALIQYLTNEEARKAISSTEAVLNNRFIRVLWHRENNEQPTLQSSAQLLLQQQQTLSHLSQ
  QHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQTSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSG
  GAGEDCQIFSTPGHPKMIYSSSNLKTPSKLCSGSKSHDVQEVLKKKQEAMKLQQDMRKKRQEVLEKQIEC
  QKMLISKLEKNKNMKPEERANIMKTLKELGEKISQLKDELKTSSAVSTPSKVKTKTEAQKELLDTELDLH
  KRLSSGEDTTELRKKLSQLQVEAARLGILPVGRGKTMSSQGRGRGRGRGGRGRGSLNHMVVDHRPKALTV
  GGFIEEEKEDLLQHFSTANQGPKFKDRRLQISWHKPKVPSISTETEEEEVKEEETETSDLFLPDDDDEDE
  DEYESRSWRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018989
• ORF Size: 3180 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018989.2
• RefSeq Size: 6555 bp
• RefSeq ORF: 3183 bp
• Locus ID: 54439
• UniProt ID: Q9P2N5
• Cytogenetics: 5q32