ADAM29 (NM_001130704) Human Tagged ORF Clone
CAT#: RG226193
- TrueORF®
ADAM29 (tGFP-tagged) - Human ADAM metallopeptidase domain 29 (ADAM29), transcript variant 3
ORF Plasmid: DDK
"NM_001130704" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ADAM29 |
Synonyms | CT73; svph1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226193 representing NM_001130704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGTTACTCCTGCTGCATTGCCTTGGGGTGTTTCTGTCCTGTTCTGGACACATCCAGGATGAGC ACCCCCAATATCACAGCCCTCCGGATGTGGTGATTCCTGTGAGGATAACTGGCACCACCAGAGGCATGAC ACCTCCAGGCTGGCTCTCCTATATCCTGCCCTTTGGAGGCCAGAAACACATTATCCACATAAAGGTCAAG AAGCTTTTGTTTTCCAAACACCTCCCTGTGTTCACCTACACAGACCAGGGTGCTATCCTTGAGGACCAGC CATTTGTCCAGAATAACTGCTACTATCATGGTTATGTGGAAGGGGACCCAGAATCCCTGGTTTCCCTCAG TACCTGTTTTGGGGGTTTTCAAGGAATATTACAGATAAATGACTTTGCTTATGAAATCAAGCCCCTAGCA TTTTCTACCACGTTTGAACATCTGGTATACAAGATGGACAGTGAGGAGAAACAATTTTCAACCATGAGAT CCGGATTTATGCAAAATGAAATAACATGCCGAATGGAATTTGAAGAAATTGATAATTCCACTCAGAAGCA AAGTTCTTATGTGGGCTGGTGGATCCATTTTAGGATTGTTGAAATTGTAGTCGTCATTGATAATTATCTG TACATTCGTTATGAAAGGAACGACTCAAAGTTGCTGGAGGATCTATATGTTATTGTTAATATAGTGGATT CCATTTTGGATGTCATTGGTGTTAAGGTGTTATTATTTGGTTTGGAGATCTGGACCAATAAAAACCTCAT TGTAGTAGATGATGTAAGGAAATCTGTGCACCTGTATTGCAAGTGGAAGTCGGAGAACATTACGCCCCGG ATGCAACATGACACCTCACATCTTTTCACAACTCTAGGATTAAGAGGGTTAAGTGGCATAGGAGCTTTTA GAGGAATGTGTACACCACACCGTAGTTGTGCAATTGTTACTTTCATGAACAAAACTTTGGGCACTTTTTC AATTGCAGTGGCTCATCATCTAGGTCATAATTTGGGCATGAACCATGATGAGGATACATGTCGTTGTTCA CAACCTAGATGCATAATGCATGAAGGCAACCCACCAATAACTAAATTTAGCAATTGTAGTTATGGTGATT TTTGGGAATATACTGTAGAGAGGACAAAGTGTTTGCTTGAAACAGTACACACAAAGGACATCTTTAATGT GAAGCGCTGTGGGAATGGTGTTGTTGAAGAAGGAGAAGAGTGTGACTGTGGACCTTTAAAGCATTGTGCA AAAGATCCCTGCTGTCTGTCAAATTGCACTCTGACTGATGGTTCTACTTGTGCTTTTGGGCTTTGTTGCA AAGACTGCAAGTTCCTACCATCAGGGAAAGTGTGTAGAAAGGAGGTCAATGAATGTGATCTTCCAGAGTG GTGCAATGGTACTTCCCATAAGTGCCCAGATGACTTTTATGTGGAAGATGGAATTCCCTGTAAGGAGAGG GGCTACTGCTATGAAAAGAGCTGTCATGACCGCAATGAACAGTGTAGGAGGATTTTTGGTGCAGGCGCAA ATACTGCAAGTGAGACTTGCTACAAAGAATTGAACACCTTAGGTGACCGTGTTGGTCACTGTGGTATCAA AAATGCTACATATATAAAGTGTAATATCTCAGATGTCCAGTGTGGAAGAATTCAGTGTGAGAATGTGACA GAAATTCCCAATATGAGTGATCATACTACTGTGCATTGGGCTCGCTTCAATGACATAATGTGCTGGAGTA CTGATTACCATTTGGGGATGAAGGGACCTGATATTGGTGAAGTGAAAGATGGAACAGAGTGTGGGATAGA TCATATATGCATCCACAGGCACTGTGTCCATATAACCATCTTGAATAGTAATTGCTCACCTGCATTTTGT AACAAGAGGGGCATCTGCAACAATAAACATCACTGCCATTGCAATTATCTGTGGGACCCTCCCAACTGCC TGATAAAAGGCTATGGAGGTAGTGTTGACAGTGGCCCACCCCCTAAGAGAAAGAAGAAAAAGAAGTTCTG TTATCTGTGTATATTGTTGCTTATTGTTTTGTTTATTTTATTATGTTGTCTTTATCGACTTTGTAAAAAA AGTAAACCAATAAAAAAGCAGCAAGATGTTCAAACTCCATCTGCAAAAGAAGAGGAAAAAATTCAGCGTC GACCTCATGAGTTACCTCCCCAGAGTCAACCTTGGGTGATGCCTTCCCAGAGTCAACCTCCTGTGACGCC TTCCCAGAGTCATCCTCAGGTGATGCCTTCCCAGAGTCAACCTCCTGTGACACCCTCCCAGAGTCAACCT CGGGTGATGCCTTCTCAGAGTCAACCTCCTGTGATGCCTTCCCAGAGTCATCCTCAGTTGACGCCTTCCC AGAGTCAACCTCCTGTGACACCCTCCCAGAGGCAACCTCAGTTGATGCCTTCCCAGAGTCAACCTCCTGT GACGCCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226193 representing NM_001130704
Red=Cloning site Green=Tags(s) MKMLLLLHCLGVFLSCSGHIQDEHPQYHSPPDVVIPVRITGTTRGMTPPGWLSYILPFGGQKHIIHIKVK KLLFSKHLPVFTYTDQGAILEDQPFVQNNCYYHGYVEGDPESLVSLSTCFGGFQGILQINDFAYEIKPLA FSTTFEHLVYKMDSEEKQFSTMRSGFMQNEITCRMEFEEIDNSTQKQSSYVGWWIHFRIVEIVVVIDNYL YIRYERNDSKLLEDLYVIVNIVDSILDVIGVKVLLFGLEIWTNKNLIVVDDVRKSVHLYCKWKSENITPR MQHDTSHLFTTLGLRGLSGIGAFRGMCTPHRSCAIVTFMNKTLGTFSIAVAHHLGHNLGMNHDEDTCRCS QPRCIMHEGNPPITKFSNCSYGDFWEYTVERTKCLLETVHTKDIFNVKRCGNGVVEEGEECDCGPLKHCA KDPCCLSNCTLTDGSTCAFGLCCKDCKFLPSGKVCRKEVNECDLPEWCNGTSHKCPDDFYVEDGIPCKER GYCYEKSCHDRNEQCRRIFGAGANTASETCYKELNTLGDRVGHCGIKNATYIKCNISDVQCGRIQCENVT EIPNMSDHTTVHWARFNDIMCWSTDYHLGMKGPDIGEVKDGTECGIDHICIHRHCVHITILNSNCSPAFC NKRGICNNKHHCHCNYLWDPPNCLIKGYGGSVDSGPPPKRKKKKKFCYLCILLLIVLFILLCCLYRLCKK SKPIKKQQDVQTPSAKEEEKIQRRPHELPPQSQPWVMPSQSQPPVTPSQSHPQVMPSQSQPPVTPSQSQP RVMPSQSQPPVMPSQSHPQLTPSQSQPPVTPSQRQPQLMPSQSQPPVTPS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130704 |
ORF Size | 2460 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130704.1, NP_001124176.1 |
RefSeq Size | 3219 bp |
RefSeq ORF | 2463 bp |
Locus ID | 11086 |
UniProt ID | Q9UKF5 |
Cytogenetics | 4q34.1 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is highly expressed in testis and may be involved in human spermatogenesis. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Jul 2008] |
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