LRIT1 (NM_015613) Human Tagged ORF Clone

CAT#: RG224949

LRIT1 (tGFP-tagged) - Human leucine-rich repeat, immunoglobulin-like and transmembrane domains 1 (LRIT1)

Product Images

Circular map for RG224949

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRIT1
• Synonyms: FIGLER9; LRRC21; PAL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224949 representing NM_015613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTGGCATTAGGCATGCTCTGGCTCTTGGCCCTTGCGTGGCCCCCCCAGGCCCGGGGCTTCTGCC
  CCTCTCAATGCAGCTGCAGCCTCCATATCATGGGTGATGGCAGCAAGGCCAGGACAGTAGTGTGCAACGA
  CCCTGACATGACCCTGCCCCCGGCGTCCATCCCCCCGGACACCTCCAGACTGCGCCTGGAGCGGACGGCC
  ATACGCAGGGTTCCTGGCGAGGCCTTCAGGCCCCTGGGCCGCCTGGAGCAGCTGTGGCTGCCTTACAACG
  CCCTCAGCGAGCTCAACGCCCTCATGCTGCGGGGTCTGCGACGCCTGCGGGAGCTGCGGCTGCCCGGGAA
  CCGCCTGGCCGCCTTCCCCTGGGCGGCGCTCAGGGACGCCCCCAAGCTGCGGCTGCTGGACCTGCAGGCC
  AACCGCCTCTCGGCTGTGCCCGCTGAGGCCGCGCGCTTCCTGGAGAACCTCACCTTCCTCGACCTCTCCA
  GCAACCAGCTGATGAGGCTCCCGCAGGAGCTCATCGTCTCCTGGGCTCACCTGGAGACCGGTATCTTTCC
  TCCCGGCCACCACCCCAGGCGGGTCCTAGGGCTACAGGACAACCCCTGGGCATGTGACTGCCGACTCTAT
  GACCTGGTTCATCTTTTGGATGGCTGGGCCCCAAACTTGGCCTTCATTGAGACTGAACTGAGATGTGCCA
  GCCCACGTAGCCTGGCCGGAGTGGCCTTCAGCCAGCTTGAACTGAGGAAGTGCCAGGGCCCAGAGCTCCA
  TCCAGGAGTGGCCAGCATCAGGTCCCTTTTGGGTGGCACAGCATTGCTACGCTGTGGAGCTACTGGAGTC
  CCTGGGCCCGAGATGAGCTGGAGGAGGGCCAATGGCAGGCCCCTTAACGGTACAGTGCACCAGGAAGTCT
  CCAGTGACGGCACGAGCTGGACTCTGCTGGGCCTGCCTGCAGTGTCCCACCTTGACTCCGGAGACTACAT
  CTGCCAAGCCAAGAACTTCCTGGGAGCCTCTGAAACTGTTATCTCCTTGATTGTCACTGAGCCGCCGACT
  TCCACAGAACACAGTGGGAGCCCAGGGGCACTATGGGCAAGGACAGGTGGCGGAGGGGAAGCTGCTGCTT
  ACAACAACAAGCTGGTGGCCAGGCATGTCCCCCAGATTCCCAAACCCGCTGTCCTGGCCACTGGACCCTC
  TGTGCCCAGCACAAAGGAGGAGCTGACCCTTGAGCACTTCCAGATGGATGCCCTGGGAGAGCTCTCTGAT
  GGGCGGGCAGGACCCTCAGAGGCACGAATGGTGAGGTCTGTGAAGGTGGTGGGGGACACTTACCACAGCG
  TGTCCTTGGTGTGGAAGGCACCCCAGGCTAAGAACACAACTGCCTTCAGTGTCCTCTACGCGGTCTTTGG
  GCAGCACAGCATGCGGCGGGTGATTGTGCAGCCTGGGAAGACCAGAGTGACCATCACTGGGCTGTTGCCC
  AAGACCAAGTATGTGGCGTGTGTCTGTGTGCAGGGCCTGGTGCCCCGGAAGGAGCAGTGTGTTATTTTCT
  CCACCAATGAAGTGGTGGATGCTGAGAACACTCAGCAGCTTATCAATGTGGTGGTGATCAGTGTGGCCAT
  CGTCATTGCCCTGCCTCTCACGCTGCTTGTCTGCTGCAGTGCTCTTCAGAAGCGCTGCCGAAAGTGCTTC
  AACAAGGACTCCACTGAGGCCACAGTTACCTACGTCAACCTAGAGAGACTGGGCTACAGCGAGGACGGCT
  TGGAGGAGCTGTCCCGGCACAGTGTCAGCGAGGCTGACAGGCTTCTCTCAGCTCGTTCCAGTGTGGACTT
  TCAGGCCTTTGGAGTCAAAGGGGGCAGGAGAATCAATGAGTACTTCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224949 representing NM_015613
  Red=Cloning site Green=Tags(s)
  
  MRVALGMLWLLALAWPPQARGFCPSQCSCSLHIMGDGSKARTVVCNDPDMTLPPASIPPDTSRLRLERTA
  IRRVPGEAFRPLGRLEQLWLPYNALSELNALMLRGLRRLRELRLPGNRLAAFPWAALRDAPKLRLLDLQA
  NRLSAVPAEAARFLENLTFLDLSSNQLMRLPQELIVSWAHLETGIFPPGHHPRRVLGLQDNPWACDCRLY
  DLVHLLDGWAPNLAFIETELRCASPRSLAGVAFSQLELRKCQGPELHPGVASIRSLLGGTALLRCGATGV
  PGPEMSWRRANGRPLNGTVHQEVSSDGTSWTLLGLPAVSHLDSGDYICQAKNFLGASETVISLIVTEPPT
  STEHSGSPGALWARTGGGGEAAAYNNKLVARHVPQIPKPAVLATGPSVPSTKEELTLEHFQMDALGELSD
  GRAGPSEARMVRSVKVVGDTYHSVSLVWKAPQAKNTTAFSVLYAVFGQHSMRRVIVQPGKTRVTITGLLP
  KTKYVACVCVQGLVPRKEQCVIFSTNEVVDAENTQQLINVVVISVAIVIALPLTLLVCCSALQKRCRKCF
  NKDSTEATVTYVNLERLGYSEDGLEELSRHSVSEADRLLSARSSVDFQAFGVKGGRRINEYFC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015613
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_015613.1, NP_056428.1
• RefSeq Size: 2100 bp
• RefSeq ORF: 1872 bp
• Locus ID: 26103
• UniProt ID: Q9P2V4
• Cytogenetics: 10q23.1
• Protein Families: Transmembrane
• Gene Summary: Possible role in phototransduction.[UniProtKB/Swiss-Prot Function]