C6ORF182 (CEP57L1) (NM_001083535) Human Tagged ORF Clone

CAT#: RG224184

CEP57L1 (tGFP-tagged) - Human centrosomal protein 57kDa-like 1 (CEP57L1), transcript variant 1

Product Images

Circular map for RG224184

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C6ORF182
• Synonyms: bA487F23.2; C6orf182; cep57R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224184 representing NM_001083535
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTGAATTAATGCATAGTATAGTAGGAAGCTATCATAAACCTCCAGAAAGAGTATTTGTTCCCT
  CATTCACCCAGAATGAACCATCTCAGAATTGCCATCCTGCAAACTTAGAAGTTACCTCTCCTAAGATACT
  TCATAGCCCAAATAGCCAAGCTCTTATTTTAGCCTTAAAAACTCTTCAGGAAAAAATTCATCGTTTAGAG
  CTGGAGAGAACACAAGCTGAAGATAACCTGAACATTCTTTCCAGAGAAGCAGCACAGTATAAGAAGGCCT
  TAGAGAATGAAACAAATGAGAGAAATCTGGCACACCAGGAGCTGATAAAGCAGAAAAAAGATATAAGTAT
  ACAGTTAAGCTCAGCCCAGTCTCGTTGTACTCTTCTAGAGAAGCAACTAGAATATACAAAGAGAATGGTT
  CTCAACGTAGAGCGAGAAAAGAACATGATCCTAGAACAACAGGCCCAGCTTCAGAGGGAAAAAGAACAAG
  ATCAGATGAAGCTGTATGCAAAACTTGAAAAGCTTGATGTCTTAGAAAAAGAGTGTTTCAGACTTACAAC
  AACTCAGAAAACTGCTGAGGACAAGATTAAACATTTAGAAGAAAAACTTAAGGAAGAAGAACATCAGCGT
  AAGCTATTTCAAGACAAAGCTTCTGAGCTTCAAACTGGACTTGAAATCAGTAAAATTATAATGTCTTCAG
  TTTCAAATCTAAAGCACTCCAAGGAAAAGAAGAAATCTTCAAAGAAAACTAAATGTATAAAGAGACGACC
  ACCTTGGCAAATTTGTTCAAAGTTTGGAGCACTGCCTTTTGTGGCTGAAAAGATGAGGCAACATCGTGAC
  CCACATATCCTTCAGAAACCTTTTAACGTGACTGAGACTAGATGTCTCCCCAAGCCTTCTAGAACAACTT
  CCTGGTGTAAAGCTATTCCTCCTGACTCAGAAAAGTCCATTTCCATTTGTGACAATTTATCTGAACTTTT
  GATGGCAATGCAAGATGAGCTGGACCAAATGAGCATGGAGCACCAAGAACTACTGAAACAAATGAAGGAA
  ACTGAAAGTCATTCAGTCTGTGACGACATAGAATGTGAACTAGAGTGTTTACTCAAGAAAATGGAAATTA
  AAGGAGAACAAATCTCCAAACTGAAGAAGCATCAAGACAGTGTCTGTAAACTGCAGCAAAAAGTTCAAAA
  CTCAAAGATGAGTGAAGCTTCAGGTATTCAGCAAGAAGACAGCTACCCTAAAGGATCAAAGAACATAAAA
  AATAGCCCCAGAAAATGTTTGACTGACACTAACCTTTTTCAGAAAAACAGCAGCTTTCATCCAATACGAG
  TTCATAATCTTCAAATGAAATTGAGAAGAGATGATATCATGTGGGAACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224184 representing NM_001083535
  Red=Cloning site Green=Tags(s)
  
  MDSELMHSIVGSYHKPPERVFVPSFTQNEPSQNCHPANLEVTSPKILHSPNSQALILALKTLQEKIHRLE
  LERTQAEDNLNILSREAAQYKKALENETNERNLAHQELIKQKKDISIQLSSAQSRCTLLEKQLEYTKRMV
  LNVEREKNMILEQQAQLQREKEQDQMKLYAKLEKLDVLEKECFRLTTTQKTAEDKIKHLEEKLKEEEHQR
  KLFQDKASELQTGLEISKIIMSSVSNLKHSKEKKKSSKKTKCIKRRPPWQICSKFGALPFVAEKMRQHRD
  PHILQKPFNVTETRCLPKPSRTTSWCKAIPPDSEKSISICDNLSELLMAMQDELDQMSMEHQELLKQMKE
  TESHSVCDDIECELECLLKKMEIKGEQISKLKKHQDSVCKLQQKVQNSKMSEASGIQQEDSYPKGSKNIK
  NSPRKCLTDTNLFQKNSSFHPIRVHNLQMKLRRDDIMWEQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083535
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083535.3
• RefSeq Size: 2915 bp
• RefSeq ORF: 1383 bp
• Locus ID: 285753
• UniProt ID: Q8IYX8
• Cytogenetics: 6q21
• Gene Summary: Centrosomal protein which may be required for microtubule attachment to centrosomes.[UniProtKB/Swiss-Prot Function]