SFI1 (NM_014775) Human Tagged ORF Clone

CAT#: RG224072

SFI1 (tGFP-tagged) - Human Sfi1 homolog, spindle assembly associated (yeast) (SFI1), transcript variant 2

Product Images

Circular map for RG224072

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SFI1
• Synonyms: hSfi1p; PISD; PPP1R139
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224072 representing NM_014775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATCTGCTCACTGAGAAGTGTATATCAAGCCACAATTTCCATCAAAAAGTGATTAAGCAGAGAA
  TGGAGAAGAAGGTTGATTCCAGGTATTTTAAGGATGGTGCAGTTAAGAAACCTTATTCTGCAAAGACACT
  GTCCAACAAGAAGTCTTCTGCATCCTTTGGGATCCGGAGGGAGTTACCTAGTACCAGTCATCTAGTGCAG
  TATCGTGGCACACATACTTGTACCCGACAGGGCCGGTTAAGAGAACTGCGCATCAGATGCGTGGCCAGAA
  AGTTCTTATATTTATGGATTCGAATGACTTTTGGAAGAGTATTTCCCTCTAAAGCCAGATTTTACTATGA
  GCAGCGATTACTACGGAAGGTCTTCGAAGAATGGAAAGAGGAGTGGTGGGTTTTCCAGCACGAGTGGAAA
  CTCTGTGTTCGAGCTGACTGTCACTACAGGTATTACCTGTACAACCTGATGTTCCAGACGTGGAAGACCT
  ATGTGCGTCAGCAGCAGGAGATGAGGAACAAGTACATTAGAGCCGAGGTTCATGATGCAAAGCAAAAGAT
  GCGACAGGCCTGGAAGTCCTGGTTGATCTACGTGGTTGTTCGTAGGACCAAACTTCAGATGCAGACTACA
  GCTCTGGAGTTTAGGCAACGGATTATCTTACGGGTGTGGTGGAGCACGTGGAGGCAGCGACTAGGACAGG
  TCCGTGTGAGCCGTGCCCTCCATGCCTCTGCTTTGAAGCACAGGGCCCTGAGCCTCCAGGTGCAGGCTTG
  GTCACAGTGGCGGGAACAGCTCCTGTATGTCCAGAAGGAGAAACAAAAGGTTGTCTCTGCAGTGAAACAT
  CATCAGCACTGGCAAAAACGGAGATTTCTAAAGGCCTGGCTTGAATACCTGCAAGTCCGCAGAGTGAAGA
  GACAGCAGAATGAGATGGCTGAGCGATTCCATCATGTCACTGTGCTCCAGATATACTTCTGTGACTGGCA
  GCAGGCCTGGGAGCGGAGGGAGAGCTTGTACGCTCACCATGCCCAGGTGGAGAAACTGGCCAGGAAGATG
  GCCCTGCGGCGCGCCTTTACTCACTGGAAACACTACATGCTGCTGTGTGCAGAAGAAGCTGCCCAGTTTG
  AGATGGCAGAAGAGCACCACAGGCACAGCCAGCTGCTGCTGCACAGGTTCTGGAACCTCTGGCGGTCTCA
  GATTGAGCAGAAAAAGGAAAGAGAGCTGCTCCCCTTACTGCATGCTGCCTGGGACCACTACAGAATAGCA
  CTGCTGTGCAAATGTATCGAATTGTGGCTACAGTATACTCAGAAGAGGCGGTACAAGCAGCTGCTACAGG
  CCAGAGCGGATGGTCATTTCCAGCAGAGAGCCCTGCCTGCTGCCTTCCACACATGGAACAGACTCTGGCG
  ATGGCGCCACCAGGAAAATGTCCTCAGTGCAAGAGCAACACGTTTCCACAGGGAGACATTAGAGAAGCAA
  GTATTTTCTCTCTGGAGGCAGAAGATGTTTCAGCATCGAGAAAACCGCCTGGCAGAGAGAATGGCCATCC
  TTCACGCAGAGCGACAGCTTCTGTATAGGTCTTGGTTCATGTGGCACCAGCAGGCAGCAGCACGTCACCA
  GGAGCAGGAGTGGCAAACAGTGGCCTGTGCCCACCACCGCCACGGGCGGCTCAAGAAAGCTTTCTGCCTC
  TGGAGGGAAAGTGCCCAAGGGCTCAGAACAGAGAGGACGGGCAGGGTGCGGGCAGCAGAATTCCACATGG
  CCCAGCTCCTGCGTTGGGCCTGGAGCCAGTGGAGGGAGTGCCTGGCCCTGCGGGGAGCGGAGCGGCAGAA
  GCTGATGCGAGCAGACCTGCACCACCAGCACAGCGTGCTGCACAGGGCGCTGCAGGCATGGGTGACTTAC
  CAGGGCAGGGTGCGAAGCATCCTCCGGGAGGTGGCAGCCAGGGAGAGCCAGCACAACAGGCAGCTGCTGC
  GCGGGGCATTACGTCGCTGGAAAGAGAACACCATGGCCCGAGTGGATGAAGCCAAAAAAACCTTTCAAGC
  AAGTACTCATTACAGAAGGACCATATGTTCCAAGGTCCTGGTCCAGTGGCGGGAAGCTGTGTCAGTGCAG
  ATGTATTACCGACAGCAGGAGGACTGTGCCATCTGGGAGGCCCAGAAGGTGCTGGACAGGGGCTGTCTGC
  GGACCTGGTTTCAGCGCTGGTGGGACTGCAGCCGGAGGTCAGCCCAGCAGAGACTGCAGCTGGAGAGGGC
  AGTGCAACACCACCACCGGCAGCTGCTGCTGGAGGGGCTGGCCCGGTGGAAGACGCACCATCTGCAGTGT
  GTCAGGAAGAGGCTCCTGCACAGGCAGAGCACCCAACTGCTGGCACAGAGACTCAGCCGGACCTGCTTCC
  GCCAGTGGAGACAACAGCTGGCAGCCAGGAGGCAGGAGCAGCGGGCGACAGTGCGGGCCCTGTGGTTCTG
  GGCCTTCTCGCTGCAGGCAAAGGTGTGGGCCACGTGGCTGGCCTTTGTACTGGAAAGGAGGAGAAAGAAG
  GCGCGGCTGCAGTGGGCGCTCCAGGCCTACCAGGGGCAGCTCCTCCAGGAGGGTGCCACGCGGCTCCTGC
  GCTTTGCAGCCAGCATGAAGGCCTCCCGGCAGCAGCTGCAGGCCCAGCAGCAGGTCCAGGCGGCTCACAG
  TCTCCATCGTGCCGTCCGCCGCTGTGCCACGCTCTGGAAACAGAAAGTGCTGGGCCGGGGCGGGAAGCCT
  CAGCCCCTGGCAGCCATCGCACCCAGCAGGAAAGTGACGTTTGAGGGTCCCCTTCTCAACCGCATTGCTG
  CTGGGGCTGGGGATGGCACCCTTGAGACCAAGAGGCCACAGGCTTCTCGGCCTCTGGGAGCTCTGGGCCG
  CCTGGCTGCTGAGGAGCCCCACGCCCTGGAGCTCAACACTGCCCACTCAGCGAGGAAGCAGCCGCGACGC
  CCACACTTCCTGTTGGAGCCTGCGCAGAGCCAGAGGCCTCAGAAGCCACAGGAACATGGCCTAGGCATGG
  CTCAGCCAGCAGCCCCCTCCCTGACGCGGCCCTTCCTGGCAGAGGCCCCGACAGCACTGGTCCCACACAG
  CCCCCTGCCTGGGGCCCTGTCAAGCGCCCCTGGCCCGAAGCAGCCCCCGACGGCAAGCACAGGCCCGGAG
  CTGCTGCTGCTGCCTCTTTCCTCCTTCATGCCCTGCGGGGCGGCTGCACCAGCCAGGGTGTCAGCACAGC
  GGGCTACTCCTAGGGATAAGCCCCCGGTCCCCTCATCCCTGGCCAGTGTCCCTGACCCCCATCTACTCCT
  TCCTGGGGACTTCTCAGCCACCAGGGCTGGGCCTGGACTTTCAACTGCAGGCAGCCTGGACCTTGAGGCT
  GAACTTGAGGAGATCCAGCAGCAACTACTGCACTACCAGACCACCAAGCAGAACCTCTGGTCCTGTCGGC
  GGCAAGCGAGCAGCCTGCGCAGGTGGCTGGAGCTGAACAGAGAGGAGCCGGGGCCTGAGGACCAGGAAGT
  AGAGCAGCAGGTGCAGAAAGAGCTGGAACAGGTGGAAATGCAGATCCAGCTGCTGGCAGAGGAGCTCCAG
  GCTCAGCGCCAGCCCATTGGCGCCTGCGTTGCCCGCATCCAGGCCCTGCGGCAGGCCCTGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224072 representing NM_014775
  Red=Cloning site Green=Tags(s)
  
  MKNLLTEKCISSHNFHQKVIKQRMEKKVDSRYFKDGAVKKPYSAKTLSNKKSSASFGIRRELPSTSHLVQ
  YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWK
  LCVRADCHYRYYLYNLMFQTWKTYVRQQQEMRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTT
  ALEFRQRIILRVWWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKH
  HQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKM
  ALRRAFTHWKHYMLLCAEEAAQFEMAEEHHRHSQLLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRIA
  LLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQ
  VFSLWRQKMFQHRENRLAERMAILHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCL
  WRESAQGLRTERTGRVRAAEFHMAQLLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTY
  QGRVRSILREVAARESQHNRQLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ
  MYYRQQEDCAIWEAQKVLDRGCLRTWFQRWWDCSRRSAQQRLQLERAVQHHHRQLLLEGLARWKTHHLQC
  VRKRLLHRQSTQLLAQRLSRTCFRQWRQQLAARRQEQRATVRALWFWAFSLQAKVWATWLAFVLERRRKK
  ARLQWALQAYQGQLLQEGATRLLRFAASMKASRQQLQAQQQVQAAHSLHRAVRRCATLWKQKVLGRGGKP
  QPLAAIAPSRKVTFEGPLLNRIAAGAGDGTLETKRPQASRPLGALGRLAAEEPHALELNTAHSARKQPRR
  PHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPE
  LLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEA
  ELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQ
  AQRQPIGACVARIQALRQALC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014775
• ORF Size: 3633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014775.4
• RefSeq Size: 4298 bp
• RefSeq ORF: 3636 bp
• Locus ID: 9814
• UniProt ID: A8K8P3
• Cytogenetics: 22q12.2
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]