NALP5 (NLRP5) (NM_153447) Human Tagged ORF Clone

CAT#: RG223877

NLRP5 (tGFP-tagged) - Human NLR family, pyrin domain containing 5 (NLRP5)

Product Images

Circular map for RG223877

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NALP5
• Synonyms: CLR19.8; MATER; NALP5; PAN11; PYPAF8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223877 representing NM_153447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTTGCAGGAGGACTTGAACTTGGAGCTGCTGCTCTGCTCTCAGCATCACCACGTGCTCTTGTCA
  CTCTTTCCACAGGTCCTACTTGCTCTATATTACCAAAGAATCCACTTTTCCCCCAAAACCTGAGCTCTCA
  GCCTTGTATCAAGATGGAAGGAGACAAATCGCTCACCTTTTCCAGCTACGGGCTGCAATGGTGTCTCTAT
  GAGCTAGACAAGGAAGAATTTCAGACATTCAAGGAATTACTAAAGAAGAAATCTTCAGAATCGACCACAT
  GCTCTATTCCACAGTTTGAAATCGAGAATGCCAACGTGGAATGTCTGGCACTCCTCTTGCATGAGTATTA
  TGGAGCATCGCTGGCCTGGGCTACGTCCATTAGCATCTTTGAAAACATGAACCTGCGAACCCTCTCGGAG
  AAGGCACGGGATGACATGAAAAGACATTCACCAGAAGATCCTGAAGCAACGATGACTGACCAAGGACCAA
  GCAAGGAAAAAGTGCCAGGAATTTCACAAGCTGTGCAACAAGATAGTGCCACAGCTGCAGAGACAAAAGA
  ACAAGAAATTTCACAAGCTATGGAACAAGAAGGTGCCACAGCAGCAGAGACAGAAGAACAAGAAATTTCA
  CAAGCTATGGAACAAGAAGGTGCCACAGCAGCAGAGACAGAAGAACAAGGACATGGAGGTGACACATGGG
  ACTACAAGAGTCACGTGATGACCAAATTCGCTGAGGAGGAGGATGTACGTCGTAGTTTTGAAAACACTGC
  TGCTGACTGGCCGGAAATGCAAACGTTGGCTGGTGCTTTTGATTCAGACCGGTGGGGCTTCCGGCCTCGC
  ACGGTGGTTCTGCACGGAAAGTCAGGAATTGGGAAATCGGCTCTAGCCAGAAGGATCGTGCTGTGCTGGG
  CGCAAGGTGGACTCTACCAGGGAATGTTCTCCTACGTCTTCTTCCTCCCCGTTAGAGAGATGCAGCGGAA
  GAAGGAGAGCAGTGTCACAGAGTTCATCTCCAGGGAGTGGCCAGACTCCCAGGCTCCGGTGACGGAGATC
  ATGTCCCGACCAGAAAGGCTGTTGTTCATCATTGACGGTTTCGATGACCTGGGCTCTGTCCTCAACAATG
  ACACAAAGCTCTGCAAAGACTGGGCTGAGAAGCAGCCTCCGTTCACCCTCATACGCAGTCTGCTGAGGAA
  GGTCCTGCTCCCTGAGTCCTTCCTGATCGTCACCGTCAGAGACGTGGGCACAGAGAAGCTCAAGTCAGAG
  GTCGTGTCTCCCCGTTACCTGTTAGTTAGAGGAATCTCCGGGGAACAAAGAATCCACTTGCTCCTTGAGC
  GCGGGATTGGTGAGCATCAGAAGACACAAGGGTTGCGTGCGATCATGAACAACCGTGAGCTGCTCGACCA
  GTGCCAGGTGCCCGCCGTGGGCTCTCTCATCTGCGTGGCCCTGCAGCTGCAGGACGTGGTGGGGGAGAGC
  GTCGCCCCCTTCAACCAAACGCTCACAGGCCTGCACGCCGCTTTTGTGTTTCATCAGCTCACCCCTCGAG
  GCGTGGTCCGGCGCTGTCTCAATCTGGAGGAAAGAGTTGTCCTGAAGCGCTTCTGCCGTATGGCTGTGGA
  GGGAGTGTGGAATAGGAAGTCAGTGTTTGACGGTGACGACCTCATGGTTCAAGGACTCGGGGAGTCTGAG
  CTCCGTGCTCTGTTTCACATGAACATCCTTCTCCCAGACAGCCACTGTGAGGAGTACTACACCTTCTTCC
  ACCTCAGTCTCCAGGACTTCTGTGCCGCCTTGTACTACGTGTTAGAGGGCCTGGAAATCGAGCCAGCTCT
  CTGCCCTCTGTACGTTGAGAAGACAAAGAGGTCCATGGAGCTTAAACAGGCAGGCTTCCATATCCACTCG
  CTTTGGATGAAGCGTTTCTTGTTTGGCCTCGTGAGCGAAGACGTAAGGAGGCCACTGGAGGTCCTGCTGG
  GCTGTCCCGTTCCCCTGGGGGTGAAGCAGAAGCTTCTGCACTGGGTCTCTCTGTTGGGTCAGCAGCCTAA
  TGCCACCACCCCAGGAGACACCCTGGACGCCTTCCACTGTCTTTTCGAGACTCAAGACAAAGAGTTTGTT
  CGCTTGGCATTAAACAGCTTCCAAGAAGTGTGGCTTCCGATTAACCAGAACCTGGACTTGATAGCATCTT
  CCTTCTGCCTCCAGCACTGTCCGTATTTGCGGAAAATTCGGGTGGATGTCAAAGGGATCTTCCCAAGAGA
  TGAGTCCGCTGAGGCATGTCCTGTGGTCCCTCTATGGATGCGGGATAAGACCCTCATTGAGGAGCAGTGG
  GAAGATTTCTGCTCCATGCTTGGCACCCACCCACACCTGCGGCAGCTGGACCTGGGCAGCAGCATCCTGA
  CAGAGCGGGCCATGAAGACCCTGTGTGCCAAGCTGAGGCATCCCACCTGCAAGATACAGACCCTGATGTT
  TAGAAATGCACAGATTACCCCTGGTGTGCAGCACCTCTGGAGAATCGTCATGGCCAACCGTAACCTAAGA
  TCCCTCAACTTGGGAGGCACCCACCTGAAGGAAGAGGATGTAAGGATGGCGTGTGAAGCCTTAAAACACC
  CAAAATGTTTGTTGGAGTCTTTGAGGCTGGATTGCTGTGGATTGACCCATGCCTGTTACCTGAAGATCTC
  CCAAATCCTTACGACCTCCCCCAGCCTGAAATCTCTGAGCCTGGCAGGAAACAAGGTGACAGACCAGGGA
  GTAATGCCTCTCAGTGATGCCTTGAGAGTCTCCCAGTGCGCCCTGCAGAAGCTGATACTGGAGGACTGTG
  GCATCACAGCCACGGGTTGCCAGAGTCTGGCCTCAGCCCTCGTCAGCAACCGGAGCTTGACACACCTGTG
  CCTATCCAACAACAGCCTGGGGAACGAAGGTGTAAATCTACTGTGTCGATCCATGAGGCTTCCCCACTGT
  AGTCTGCAGAGGCTGATGCTGAATCAGTGCCACCTGGACACGGCTGGCTGTGGTTTTCTTGCACTTGCGC
  TTATGGGTAACTCATGGCTGACGCACCTGAGCCTTAGCATGAACCCTGTGGAAGACAATGGCGTGAAGCT
  TCTGTGCGAGGTCATGAGAGAACCATCTTGTCATCTCCAGGACCTGGAGTTGGTAAAGTGTCATCTCACC
  GCCGCGTGCTGTGAGAGTCTGTCCTGTGTGATCTCGAGGAGCAGACACCTGAAGAGCCTGGATCTCACGG
  ACAATGCCCTGGGTGACGGTGGGGTTGCTGCGCTGTGCGAGGGACTGAAGCAAAAGAACAGTGTTCTGGC
  GAGACTCGGGTTGAAGGCATGTGGACTGACTTCTGATTGCTGTGAGGCACTCTCCTTGGCCCTTTCCTGC
  AACCGGCATCTGACCAGTCTAAACCTGGTGCAGAATAACTTCAGTCCCAAAGGAATGATGAAGCTGTGTT
  CGGCCTTTGCCTGTCCCACGTCTAACTTACAGATAATTGGGCTGTGGAAATGGCAGTACCCTGTGCAAAT
  AAGGAAGCTGCTGGAGGAAGTGCAGCTACTCAAGCCCCGAGTCGTAATTGACGGTAGTTGGCATTCTTTT
  GATGAAGATGACCGGTACTGGTGGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223877 representing NM_153447
  Red=Cloning site Green=Tags(s)
  
  MKVAGGLELGAAALLSASPRALVTLSTGPTCSILPKNPLFPQNLSSQPCIKMEGDKSLTFSSYGLQWCLY
  ELDKEEFQTFKELLKKKSSESTTCSIPQFEIENANVECLALLLHEYYGASLAWATSISIFENMNLRTLSE
  KARDDMKRHSPEDPEATMTDQGPSKEKVPGISQAVQQDSATAAETKEQEISQAMEQEGATAAETEEQEIS
  QAMEQEGATAAETEEQGHGGDTWDYKSHVMTKFAEEEDVRRSFENTAADWPEMQTLAGAFDSDRWGFRPR
  TVVLHGKSGIGKSALARRIVLCWAQGGLYQGMFSYVFFLPVREMQRKKESSVTEFISREWPDSQAPVTEI
  MSRPERLLFIIDGFDDLGSVLNNDTKLCKDWAEKQPPFTLIRSLLRKVLLPESFLIVTVRDVGTEKLKSE
  VVSPRYLLVRGISGEQRIHLLLERGIGEHQKTQGLRAIMNNRELLDQCQVPAVGSLICVALQLQDVVGES
  VAPFNQTLTGLHAAFVFHQLTPRGVVRRCLNLEERVVLKRFCRMAVEGVWNRKSVFDGDDLMVQGLGESE
  LRALFHMNILLPDSHCEEYYTFFHLSLQDFCAALYYVLEGLEIEPALCPLYVEKTKRSMELKQAGFHIHS
  LWMKRFLFGLVSEDVRRPLEVLLGCPVPLGVKQKLLHWVSLLGQQPNATTPGDTLDAFHCLFETQDKEFV
  RLALNSFQEVWLPINQNLDLIASSFCLQHCPYLRKIRVDVKGIFPRDESAEACPVVPLWMRDKTLIEEQW
  EDFCSMLGTHPHLRQLDLGSSILTERAMKTLCAKLRHPTCKIQTLMFRNAQITPGVQHLWRIVMANRNLR
  SLNLGGTHLKEEDVRMACEALKHPKCLLESLRLDCCGLTHACYLKISQILTTSPSLKSLSLAGNKVTDQG
  VMPLSDALRVSQCALQKLILEDCGITATGCQSLASALVSNRSLTHLCLSNNSLGNEGVNLLCRSMRLPHC
  SLQRLMLNQCHLDTAGCGFLALALMGNSWLTHLSLSMNPVEDNGVKLLCEVMREPSCHLQDLELVKCHLT
  AACCESLSCVISRSRHLKSLDLTDNALGDGGVAALCEGLKQKNSVLARLGLKACGLTSDCCEALSLALSC
  NRHLTSLNLVQNNFSPKGMMKLCSAFACPTSNLQIIGLWKWQYPVQIRKLLEEVQLLKPRVVIDGSWHSF
  DEDDRYWWKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153447
• ORF Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153447.4, NP_703148.4
• RefSeq Size: 3885 bp
• RefSeq ORF: 3603 bp
• Locus ID: 126206
• UniProt ID: P59047
• Cytogenetics: 19q13.43
• Gene Summary: The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). Expression of this gene is restricted to the oocyte. A mouse gene that encodes a maternal oocyte protein, similar to this encoded protein, is required for normal early embryogenesis. [provided by RefSeq, Jul 2008]