TMPRSS11A (NM_182606) Human Tagged ORF Clone

CAT#: RG221395

TMPRSS11A (tGFP-tagged) - Human transmembrane protease, serine 11A (TMPRSS11A), transcript variant 1

Product Images

Circular map for RG221395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMPRSS11A
• Synonyms: ECRG1; HATL1; HESP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221395 representing NM_182606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTATCGGACAGTAGGATTTGGCACCCGAAGCAGAAATCTGAAGCCATGGATGATTGCCGTTCTCA
  TTGTGTTGTCCCTGACAGTGGTGGCAGTGACCATAGGTCTCCTGGTTCACTTCCTAGTATTTGACCAAAA
  AAAGGAGTACTATCATGGCTCCTTTAAAATTTTAGATCCACAAATCAATAACAATTTCGGACAAAGCAAC
  ACATATCAACTTAAGGACTTACGAGAGACGACCGAAAATTTGGTGAGTCAGGTGGATGAGATATTTATAG
  ATTCAGCCTGGAAGAAAAATTATATCAAGAACCAAGTAGTCAGACTGACTCCAGAGGAAGATGGTGTGAA
  AGTAGATGTCATTATGGTGTTCCAGTTCCCCTCTACTGAACAAAGGGCAGTAAGAGAGAAGAAAATCCAA
  AGCATCTTAAATCAGAAGATAAGGAATTTAAGAGCCTTGCCAATAAATGCCTCATCAGTTCAAGTTAATG
  CAATGAGCTCATCAACAGGGGAGTTAACTGTCCAAGCAAGTTGTGGTAAACGAGTTGTTCCATTAAACGT
  CAACAGAATAGCATCTGGAGTCATTGCACCCAAGGCGGCCTGGCCTTGGCAAGCTTCCCTTCAGTATGAT
  AACATCCATCAGTGTGGGGCCACCTTGATTAGTAACACATGGCTTGTCACTGCAGCACACTGCTTCCAGA
  AGTATAAAAATCCACATCAATGGACTGTTAGTTTTGGAACAAAAATCAACCCTCCCTTAATGAAAAGAAA
  TGTCAGAAGATTTATTATCCATGAGAAGTACCGCTCTGCAGCAAGAGAGTACGACATTGCTGTTGTGCAG
  GTCTCTTCCAGAGTCACCTTTTCGGATGACATACGCCGGATTTGTTTGCCAGAAGCCTCTGCATCCTTCC
  AACCAAATTTGACTGTCCACATCACAGGATTTGGAGCACTTTACTATGGTGGGGAATCCCAAAATGATCT
  CCGAGAAGCCAGAGTGAAAATCATAAGTGACGATGTCTGCAAGCAACCACAGGTGTATGGCAATGATATA
  AAACCTGGAATGTTCTGTGCCGGATATATGGAAGGAATTTATGATGCCTGCAGGGGTGATTCTGGGGGAC
  CTTTAGTCACAAGGGATCTGAAAGATACGTGGTATCTCATTGGAATTGTAAGCTGGGGAGATAACTGTGG
  TCAAAAGGACAAGCCTGGAGTCTACACACAAGTGACTTATTACCGAAACTGGATTGCTTCAAAAACAGGC
  ATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221395 representing NM_182606
  Red=Cloning site Green=Tags(s)
  
  MMYRTVGFGTRSRNLKPWMIAVLIVLSLTVVAVTIGLLVHFLVFDQKKEYYHGSFKILDPQINNNFGQSN
  TYQLKDLRETTENLVSQVDEIFIDSAWKKNYIKNQVVRLTPEEDGVKVDVIMVFQFPSTEQRAVREKKIQ
  SILNQKIRNLRALPINASSVQVNAMSSSTGELTVQASCGKRVVPLNVNRIASGVIAPKAAWPWQASLQYD
  NIHQCGATLISNTWLVTAAHCFQKYKNPHQWTVSFGTKINPPLMKRNVRRFIIHEKYRSAAREYDIAVVQ
  VSSRVTFSDDIRRICLPEASASFQPNLTVHITGFGALYYGGESQNDLREARVKIISDDVCKQPQVYGNDI
  KPGMFCAGYMEGIYDACRGDSGGPLVTRDLKDTWYLIGIVSWGDNCGQKDKPGVYTQVTYYRNWIASKTG
  I
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182606
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182606.2, NP_872412.2
• RefSeq Size: 2429 bp
• RefSeq ORF: 1266 bp
• Locus ID: 339967
• UniProt ID: Q6ZMR5
• Cytogenetics: 4q13.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Probable serine protease which may play a role in cellular senescence. Overexpression inhibits cell growth and induce G1 cell cycle arrest.[UniProtKB/Swiss-Prot Function]