TMEM150C (NM_001080506) Human Tagged ORF Clone

CAT#: RG219737

TMEM150C (tGFP-tagged) - Human transmembrane protein 150C (TMEM150C)

Product Images

Circular map for RG219737

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM150C
• Synonyms: TTN3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219737 representing NM_001080506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGGAAGAAATGCAGCGTATGGATGTTCCTACCTCTTGTATTTACTTTGTTTACTTCAGCTGGAT
  TGTGGATAGTATACTTCATAGCTGTGGAAGATGACAAAATTTTACCATTAAATTCAGCTGAAAGGAAACC
  TGGTGTGAAGCATGCACCATATATAAGCATTGCAGGTGATGATCCTCCTGCAAGCTGTGTGTTTAGTCAA
  GTTATGAACATGGCAGCCTTCCTAGCCCTTGTGGTAGCTGTTCTGCGCTTCATACAACTGAAACCGAAGG
  TTTTAAACCCGTGGCTGAATATTAGTGGATTGGTGGCTCTGTGTCTGGCTTCCTTCGGAATGACCTTACT
  TGGTAATTTTCAGCTCACAAATGATGAAGAAATCCATAACGTCGGAACTTCCTTGACCTTTGGATTTGGC
  ACATTGACCTGCTGGATCCAGGCTGCGCTGACACTCAAGGTCAACATCAAGAATGAAGGACGGAGAGTTG
  GAATTCCACGGGTTATTCTGTCGGCATCTATCACTCTCTGTGTGGTCCTCTACTTCATCCTCATGGCCCA
  AAGCATCCACATGTATGCAGCCAGGGTCCAGTGGGGCCTGGTCATGTGCTTCCTGTCTTATTTTGGCACC
  TTTGCCGTGGAGTTCCGGCATTACCGCTATGAGATTGTTTGCTCTGAGTACCAGGAGAATTTCCTAAGCT
  TCTCAGAAAGCCTGTCAGAAGCTTCTGAATATCAGACTGACCAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219737 representing NM_001080506
  Red=Cloning site Green=Tags(s)
  
  MDGKKCSVWMFLPLVFTLFTSAGLWIVYFIAVEDDKILPLNSAERKPGVKHAPYISIAGDDPPASCVFSQ
  VMNMAAFLALVVAVLRFIQLKPKVLNPWLNISGLVALCLASFGMTLLGNFQLTNDEEIHNVGTSLTFGFG
  TLTCWIQAALTLKVNIKNEGRRVGIPRVILSASITLCVVLYFILMAQSIHMYAARVQWGLVMCFLSYFGT
  FAVEFRHYRYEIVCSEYQENFLSFSESLSEASEYQTDQV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080506
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080506.3
• RefSeq Size: 1888 bp
• RefSeq ORF: 750 bp
• Locus ID: 441027
• UniProt ID: B9EJG8
• Cytogenetics: 4q21.22
• Gene Summary: This gene encodes a transmembrane protein component of a mechanosensitve ion channel that is activated by mechanical stimuli in various cell types and confers slowly adapting, mechanically activated currents in dorsal root ganglion neurons. Mechanically activated ion channels are sensors that are critical for hearing, touch, pain, and blood pressure regulation. [provided by RefSeq, Jul 2017]