PHD finger protein 6 (PHF6) (NM_001015877) Human Tagged ORF Clone
CAT#: RG218127
- TrueORF®
PHF6 (tGFP-tagged) - Human PHD finger protein 6 (PHF6), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001015877" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PHD finger protein 6 |
Synonyms | BFLS; BORJ; CENP-31 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG218127 representing NM_001015877
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAGCTCAGTTGAACAGAAAAAAGGGCCTACAAGACAGCGCAAATGTGGCTTTTGTAAGTCAAATA GAGACAAGGAATGTGGACAGTTACTAATATCTGAAAACCAGAAGGTGGCAGCGCACCATAAGTGCATGCT CTTTTCATCTGCTTTGGTATCATCACACTCTGATAATGAAAGTCTTGGTGGATTTTCTATTGAAGATGTC CAAAAGGAAATTAAAAGAGGCACGAAGCTGATGTGTTCTTTGTGCCATTGTCCTGGAGCAACAATTGGTT GTGATGTGAAAACATGTCACAGGACATACCACTACCACTGTGCATTGCATGATAAAGCTCAAATACGAGA GAAACCTTCACAAGGAATTTACATGGTCTATTGCCGAAAACACAAGAAAACTGCACATAACTCCGAAGCT GATTTAGAAGAAAGTTTTAATGAACATGAACTGGAGCCCTCATCACCTAAAAGTAAAAAGAAAAGTCGCA AAGGAAGGCCAAGAAAAACTAATTTTAAAGGGCTGTCAGAAGATACCAGGTCCACATCCTCCCATGGAAC AGATGAAATGGAAAGTAGTTCCTATAGAGATAGGTCTCCACACAGAAGCAGCCCTAGTGACACCAGGCCT AAATGTGGATTTTGCCATGTAGGGGAGGAAGAAAATGAAGCACGAGGAAAACTGCATATATTTAATGCCA AGAAGGCAGCTGCCCATTATAAGTGCATGTTGTTTTCTTCTGGCACAGTCCAGCTCACAACAACATCAAG AGCAGAATTTGGAGACTTTGATATTAAAACTGTACTTCAGGAGATTAAACGAGGAAAAAGAATGAAATGT ACACTTTGCAGTCAGCCTGGTGCTACTATTGGATGTGAAATAAAAGCCTGTGTTAAGACTTACCATTACC ACTGTGGAGTACAAGACAAAGCTAAATACATTGAAAATATGTCACGAGGAATTTACAAACTATACTGTAA AAATCATAGTGGAAATGATGAGAGAGATGAAGAAGATGAGGAACGAGAGAGTAAAAGCCGAGGAAAAGTA GAAATTGATCAGCAACAACTAACTCAGCAGCAACTTAATGGAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218127 representing NM_001015877
Red=Cloning site Green=Tags(s) MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDV QKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMVYCRKHKKTAHNSEA DLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRP KCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMKC TLCSQPGATIGCEIKACVKTYHYHCGVQDKAKYIENMSRGIYKLYCKNHSGNDERDEEDEERESKSRGKV EIDQQQLTQQQLNGN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001015877 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001015877.2 |
RefSeq Size | 4442 bp |
RefSeq ORF | 1098 bp |
Locus ID | 84295 |
UniProt ID | Q8IWS0 |
Cytogenetics | Xq26.2 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by cognitive disability, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate splicing results in multiple transcript variants, encoding different isoforms. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218127 | PHF6 (Myc-DDK-tagged)-Human PHD finger protein 6 (PHF6), transcript variant 1 |
USD 457.00 |
|
RC218127L3 | Lenti ORF clone of Human PHD finger protein 6 (PHF6), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC218127L4 | Lenti ORF clone of Human PHD finger protein 6 (PHF6), transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC302002 | PHF6 (untagged)-Human PHD finger protein 6 (PHF6), transcript variant 1 |
USD 503.00 |
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