SLC12A6 (NM_001042496) Human Tagged ORF Clone

CAT#: RG215055

SLC12A6 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 5

Product Images

Circular map for RG215055

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC12A6
• Synonyms: ACCPN; KCC3; KCC3A; KCC3B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215055 representing NM_001042496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAGTTCGGTTCATGGTGACACCGACAAAGATCGATGACATTCCAGGTTTGTCAGACACCAGTC
  CGGACCTCAGCTCTCGATCTAGTTCCCGAGTAAGATTTAGCTCCCGGGAAAGCGTGCCTGAAACAAGCCG
  GAGTGAGCCTATGAGTGAGATGTCTGGGGCCACCACTTCGCTGGCAACTGTTGCACTGGATCCACCCAGT
  GACCGGACTTCTCACCCCCAGGATGTCATCGAGGACCTGAGTCAGAACTCCATCACAGGGGAACACAGCC
  AACTGTTAGACGACGGACATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAATTATGAAGAAGGAGA
  TGAATATTTTGATAAAAATTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGAAGGTGTCTTCCCTC
  CTCAACCGCATGGCCAATTACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGAGGCAGAAAACATCA
  CTGAAGGGAAAAAGAAGCCCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTCTACCTCCCATGTCT
  ACAAAATATTTTTGGAGTGATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAGCTGGAGTTCTTCAG
  GCTTTTGCAATTGTCCTTATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCATGAGTGCCATTGCCA
  CTAATGGAGTGGTGCCAGCTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGCCCAGAGTTTGGTGG
  GGCTGTTGGCCTCTGCTTTTATCTTGGTACCACATTTGCAGCAGCCATGTACATCCTTGGTGCCATTGAA
  ATCTTTCTGGTCTATATCGTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACTCAAGGAATCAGCAG
  CCATGCTAAATAACATGCGTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTAGTGGTATTTATCGG
  CGTACGCTATGTGAACAAGTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCATCTTGGCCATCTAT
  GCTGGAGCCATCAAGTCTTCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGGTAACCGCACCCTTT
  CATCAAGACACATTGACGTTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTCCCATCAAAGTTATG
  GGGATTCTTCTGTAACTCGAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTGTTCACAATAACGTC
  ACTTCAATCCAGGGCATTCCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTGGAGTAATTACCTAC
  CCAAGGGAGAGATCATCGAAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGCTTAAACCATGAATA
  TGTTCTTGTTGACATCACCACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCTCTGTTACAGGTATC
  ATGGCTGGATCAAACAGATCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGATTGGTACTATCCTTG
  CCATCCTGACCACCTCCTTTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGGGTTGT
  TCTCAGAGACAAGTTCGGTGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTATCTTGGCCATCCCCA
  TGGGTGATTGTTATTGGCTCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCTCACAGGTGCACCGA
  GGCTGCTACAAGCTATTGCCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGCCACAGCAAAGCCAA
  TGGGGAACCTACCTGGGCTTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATACTCATTGCCTCCCTG
  GATCTTGTGGCCCCAATTCTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTAAACTTGGCATGTGCCT
  TGCAAACATTACTTCGAACACCCAACTGGAGACCCCGATTCCGCTACTACCATTGGGCCCTTTCTTTCAT
  GGGAATGAGTATCTGTCTGGCTCTGATGTTCATTTCTTCCTGGTATTATGCCATTGTAGCCATGGTAATA
  GCTGGTATGATCTACAAGTACATTGAATACCAAGGAGCTGAGAAAGAATGGGGTGATGGTATCCGTGGGC
  TGTCCCTCAGTGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGAAGGACCTCCACACACTAAAAACTGGAG
  GCCTCAGTTGCTTGTATTACTGAAACTAGATGAAGACTTACATGTCAAGCATCCTCGCCTCCTCACCTTT
  GCCTCACAGCTCAAAGCAGGAAAAGGTCTCACTATTGTGGGCTCTGTCATCGTGGGGAACTTCCTAGAGA
  ACTACGGTGAAGCTTTAGCTGCTGAGCAGACCATAAAGCACCTAATGGAGGCAGAGAAGGTAAAAGGATT
  CTGCCAGCTGGTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCCCACCTCATCCAGTCATGTGGCCTTGGG
  GGCATGAAGCACAACACGGTGGTGATGGGCTGGCCTAATGGCTGGCGTCAAAGCGAAGATGCCCGCGCTT
  GGAAGACTTTTATTGGCACAGTTCGAGTGACAACTGCTGCCCATCTTGCACTGCTGGTGGCTAAAAACAT
  CTCCTTCTTTCCCAGCAATGTGGAGCAATTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGAT
  GGGGGGATGCTTATGCTACTACCATTCCTACTGAAACAGCACAAGGTGTGGCGAAAGTGCAGCATACGGA
  TCTTCACAGTAGCCCAATTAGAAGACAACAGTATCCAAATGAAGAAGGACCTAGCCACCTTCCTATATCA
  CTTACGCATTGAGGCGGAGGTAGAAGTGGTGGAGATGCATGACAGTGATATATCAGCATATACTTACGAG
  CGCACTTTGATGATGGAACAAAGGTCCCAGATGCTCCGGCACATGCGGCTATCCAAAACAGAGCGAGACA
  GAGAGGCACAATTGGTGAAAGACCGAAACTCAATGCTACGATTGACCAGCATTGGCTCTGATGAGGACGA
  AGAGACAGAAACCTATCAGGAGAAGGTGCACATGACTTGGACAAAAGACAAGTACATGGCATCCCGGGGA
  CAAAAAGCGAAGTCAATGGAAGGATTCCAGGACCTGCTTAACATGCGTCCGGACCAGTCCAATGTGAGGC
  GGATGCATACAGCAGTGAAACTCAACGAGGTTATAGTTAACAAGTCCCATGAAGCAAAGCTGGTTTTATT
  GAATATGCCAGGGCCACCCCGAAACCCTGAGGGTGATGAAAACTACATGGAGTTCCTAGAGGTGCTTACC
  GAGGGACTAGAGCGAGTCCTACTTGTCCGGGGTGGTGGCAGTGAAGTGATCACCATTTATTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215055 representing NM_001042496
  Red=Cloning site Green=Tags(s)
  
  MASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDPPS
  DRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSL
  LNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQ
  AFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIE
  IFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIY
  AGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNV
  TSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGI
  MAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSP
  WVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASL
  DLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVI
  AGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTF
  ASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLG
  GMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHD
  GGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYE
  RTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRG
  QKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLT
  EGLERVLLVRGGGSEVITIYS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042496
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042496.2
• RefSeq Size: 7754 bp
• RefSeq ORF: 3426 bp
• Locus ID: 9990
• UniProt ID: Q9UHW9
• Cytogenetics: 15q14
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]