JIP3 (MAPK8IP3) (NM_001040439) Human Tagged ORF Clone

CAT#: RG213840

MAPK8IP3 (tGFP-tagged) - Human mitogen-activated protein kinase 8 interacting protein 3 (MAPK8IP3), transcript variant 2

Product Images

Circular map for RG213840

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: JIP3
• Synonyms: JIP-3; JIP3; JSAP1; NEDBA; syd; SYD2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213840 representing NM_001040439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGATCCAGATGGACGAGGGCGGCGGCGTGGTGGTGTACCAGGACGACTACTGCTCCGGCTCGG
  TGATGTCGGAGCGGGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATCCACTGCTA
  CGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTAGACTCGGTGCTC
  AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTGCTGCGCGAGGACAACGAGCAGCTGCTCACCCAGT
  ACGAGCGTGAGAAGGCGCTGCGCAGGCAGGCGGAGGAGAAATTCATTGAGTTTGAAGATGCTCTGGAACA
  AGAGAAGAAAGAGCTGCAAATCCAGGTGGAGCACTACGAGTTCCAGACGCGCCAGCTGGAGCTGAAGGCC
  AAGAACTATGCCGATCAGATTTCCCGGTTGGAGGAGCGGGAGTCGGAGATGAAGAAGGAGTACAATGCCC
  TGCACCAGCGGCACACAGAGATGATACAGACCTACGTGGAGCACATTGAGAGGTCCAAGATGCAGCAGGT
  CGGAGGAAACAGCCAGACCGAGAGCAGCCTGCCGGGGCGGAGGAAGGAGCGCCCCACCTCCCTGAACGTG
  TTCCCCCTGGCTGACGGCACGGTACGTGCACAGATCGGGGGCAAGCTCGTGCCTGCGGGGGACCACTGGC
  ACCTGAGTGACCTCGGCCAGCTGCAGTCCAGCTCCAGCTACCAGTGTCCACAGGATGAAATGTCCGAGTC
  AGGCCAGTCCTCGGCGGCCGCCACACCCAGCACCACAGGCACCAAGTCCAACACGCCCACATCCTCCGTG
  CCCTCGGCCGCCGTCACACCCCTCAACGAGAGCCTGCAGCCCCTGGGGGACTATGGCGTGGGCTCCAAGA
  ACAGCAAGCGTGCCCGGGAGAAGCGCGACAGCCGCAACATGGAAGTACAGGTCACCCAGGAGATGCGCAA
  CGTCAGTATAGGCATGGGCAGCAGTGACGAGTGGTCTGATGTTCAAGACATTATTGACTCCACGCCAGAG
  CTGGACATGTGTCCAGAGACCCGCCTGGACCGCACAGGAAGCAGCCCAACCCAGGGCATCGTGAACAAAG
  CTTTCGGCATCAACACCGACTCCCTGTACCATGAGCTGTCGACGGCAGGGTCTGAGGTCATCGGGGATGT
  GGACGAAGGGGCCGACCTCCTAGGGGAGTTCTCAGGAATGGGCAAAGAAGTGGGGAATCTGCTACTGGAA
  AACTCACAGCTTCTGGAAACCAAAAACGCCTTGAATGTGGTGAAGAATGACCTGATTGCCAAGGTCGACC
  AGCTGTCCGGGGAGCAGGAGGTGCTGAGGGGCGAGTTGGAGGCTGCTAAGCAGGCCAAAGTCAAGCTGGA
  AAACCGTATCAAGGAGCTGGAAGAGGAACTGAAAAGAGTGAAGTCCGAGGCCATCATCGCCCGCCGTGAA
  CCCAAAGAAGAGGCGGAGGATGTAAGCAGCTATCTCTGTACAGAATCGGACAAAATCCCCATGGCCCAGC
  GCCGCCGCTTCACGCGGGTGGAGATGGCCCGTGTGCTCATGGAGCGGAACCAGTACAAGGAGCGGCTGAT
  GGAGCTGCAGGAGGCTGTGCGGTGGACTGAGATGATCAGAGCGTCCCGAGAGCACCCATCCGTCCAGGAG
  AAGAAGAAGTCGACCATCTGGCAGTTCTTCAGCCGCCTCTTCAGCTCTTCCTCCAGCCCCCCTCCGGCCA
  AGCGCCCCTATCCCTCGGTGAACATCCACTACAAGTCACCCACCACTGCCGGCTTCAGCCAGCGCCGCAA
  CCATGCCATGTGCCCGATCTCGGCAGGCAGCCGGCCCCTGGAATTCTTCCCTGACGACGACTGCACGTCC
  TCCGCCCGTCGAGAGCAGAAGCGCGAGCAGTACCGCCAGGTGCGTGAGCACGTGCGTAACGACGACGGCC
  GTCTGCAGGCCTGCGGCTGGAGCCTGCCCGCCAAGTACAAGCAGCTGAGTCCCAACGGGGGCCAGGAGGA
  CACGCGGATGAAGAACGTGCCGGTGCCGGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCCACCATGAAG
  CTGTGGTGTGCCGCGGGCGTCAACCTGAGCGGGTGGAGGCCCAATGAGGACGACGCTGGGAATGGAGTCA
  AGCCAGCGCCAGGCCGCGATCCCCTGACCTGCGACCGCGAAGGAGACGGCGAGCCCAAGAGCGCCCACAC
  GTCTCCCGAGAAGAAGAAGGCCAAGGAGCTCCCTGAAATGGACGCCACCTCCAGCCGGGTGTGGATCCTG
  ACCAGCACCCTGACCACCAGCAAGGTGGTGATCATCGACGCCAACCAGCCGGGCACGGTGGTGGACCAGT
  TCACCGTCTGCAACGCGCACGTGCTGTGCATCTCCAGCATCCCCGCGGCCAGCGACAGCGACTACCCTCC
  CGGGGAGATGTTCCTGGACAGCGACGTGAACCCAGAGGACCCGGGCGCAGATGGCGTGCTGGCCGGTATC
  ACCCTGGTGGGCTGTGCCACCCGCTGCAACGTGCCGCGGAGCAACTGCTCCTCCCGAGGGGACACCCCAG
  TGCTAGACAAGGGGCAGGGGGAGGTGGCCACCATCGCCAACGGGAAGGTCAACCCGTCCCAGTCCACAGA
  GGAGGCCACAGAGGCCACGGAGGTGCCAGACCCTGGGCCCAGCGAGCCAGAGACAGCCACATTGCGGCCC
  GGGCCTCTCACAGAGCACGTCTTCACTGACCCAGCCCCGACCCCGTCCTCTGGCCCCCAGCCTGGCAGCG
  AGAACGGGCCAGAGCCTGACAGCAGCAGCACACGGCCAGAGCCAGAGCCCAGCGGGGACCCCACGGGAGC
  AGGCAGCAGTGCTGCACCCACCATGTGGCTGGGAGCCCAGAACGGCTGGCTCTATGTGCACTCGGCTGTG
  GCCAACTGGAAGAAGTGCCTGCACTCCATCAAGCTGAAGGATTCTGTGCTGAGCCTGGTGCATGTCAAAG
  GCCGTGTGCTGGTGGCTCTGGCGGACGGGACCCTGGCCATCTTCCACCGTGGTGAAGATGGCCAGTGGGA
  TCTGAGCAACTATCACCTAATGGACCTGGGCCACCCGCACCACTCCATCCGCTGCATGGCTGTTGTGTAC
  GACCGCGTGTGGTGTGGCTACAAGAACAAGGTGCACGTCATCCAGCCCAAGACCATGCAGATAGAGAAGT
  CATTTGACGCCCACCCGCGGCGGGAGAGCCAGGTGCGGCAGCTGGCGTGGATCGGCGATGGCGTATGGGT
  GTCCATCCGCCTGGACTCCACCCTGAGGCTCTACCATGCACACACGCACCAGCATCTACAGGACGTGGAC
  ATTGAGCCCTACGTCAGCAAGATGCTAGGCACTGGCAAGCTGGGTTTCTCCTTCGTACGCATCACGGCCC
  TGCTTGTCGCGGGCAGCCGGCTCTGGGTGGGCACCGGCAACGGAGTGGTCATCTCCATCCCCCTGACAGA
  GACTGTGGTCCTGCACCGAGGCCAGCTCCTGGGGCTCCGAGCCAATAAGACATCCCCCACCTCTGGGGAG
  GGCGCCCGTCCCGGGGGCATCATCCACGTGTATGGCGATGACAGCAGTGACAGGGCGGCCAGCAGCTTCA
  TCCCCTACTGCTCCATGGCCCAGGCCCAGCTATGCTTCCATGGGCACCGCGATGCCGTGAAGTTCTTTGT
  CTCGGTGCCAGGGAACGTGCTGGCCACCCTGAATGGGAGTGTGCTGGACAGCCCAGCCGAGGGCCCTGGG
  CCAGCTGCCCCTGCCTCGGAGGTCGAGGGCCAGAAGCTGCGGAACGTGCTGGTGCTGAGCGGCGGGGAGG
  GCTACATCGACTTCCGCATTGGAGACGGAGAGGACGACGAGACGGAGGAGGGCGCAGGGGACATGAGCCA
  GGTGAAGCCCGTGCTGTCCAAGGCAGAGCGCAGTCACATCATCGTGTGGCAGGTGTCCTACACCCCCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213840 representing NM_001040439
  Red=Cloning site Green=Tags(s)
  
  MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVL
  SENQEHEVELELLREDNEQLLTQYEREKALRRQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKA
  KNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGNSQTESSLPGRRKERPTSLNV
  FPLADGTVRAQIGGKLVPAGDHWHLSDLGQLQSSSSYQCPQDEMSESGQSSAAATPSTTGTKSNTPTSSV
  PSAAVTPLNESLQPLGDYGVGSKNSKRAREKRDSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTPE
  LDMCPETRLDRTGSSPTQGIVNKAFGINTDSLYHELSTAGSEVIGDVDEGADLLGEFSGMGKEVGNLLLE
  NSQLLETKNALNVVKNDLIAKVDQLSGEQEVLRGELEAAKQAKVKLENRIKELEEELKRVKSEAIIARRE
  PKEEAEDVSSYLCTESDKIPMAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQE
  KKKSTIWQFFSRLFSSSSSPPPAKRPYPSVNIHYKSPTTAGFSQRRNHAMCPISAGSRPLEFFPDDDCTS
  SARREQKREQYRQVREHVRNDDGRLQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPTMK
  LWCAAGVNLSGWRPNEDDAGNGVKPAPGRDPLTCDREGDGEPKSAHTSPEKKKAKELPEMDATSSRVWIL
  TSTLTTSKVVIIDANQPGTVVDQFTVCNAHVLCISSIPAASDSDYPPGEMFLDSDVNPEDPGADGVLAGI
  TLVGCATRCNVPRSNCSSRGDTPVLDKGQGEVATIANGKVNPSQSTEEATEATEVPDPGPSEPETATLRP
  GPLTEHVFTDPAPTPSSGPQPGSENGPEPDSSSTRPEPEPSGDPTGAGSSAAPTMWLGAQNGWLYVHSAV
  ANWKKCLHSIKLKDSVLSLVHVKGRVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVY
  DRVWCGYKNKVHVIQPKTMQIEKSFDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVD
  IEPYVSKMLGTGKLGFSFVRITALLVAGSRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGE
  GARPGGIIHVYGDDSSDRAASSFIPYCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPAEGPG
  PAAPASEVEGQKLRNVLVLSGGEGYIDFRIGDGEDDETEEGAGDMSQVKPVLSKAERSHIIVWQVSYTPE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040439
• ORF Size: 3990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040439.2
• RefSeq Size: 5635 bp
• RefSeq ORF: 3993 bp
• Locus ID: 23162
• UniProt ID: Q9UPT6
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]