DHX34 (NM_014681) Human Tagged ORF Clone

CAT#: RG213162

DHX34 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 34 (DHX34)

Product Images

Circular map for RG213162

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DHX34
• Synonyms: DDX34; HRH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213162 representing NM_014681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCTCCTAGAACAAGGGAGGGCAGGGATCGCCGAGACCACCACCGGGCTCCCAGCGAGGAAGAGG
  CCTTGGAGAAATGGGACTGGAATTGTCCAGAGACGCGTCGCCTCTTGGAAGATGCCTTCTTCCGTGAAGA
  GGATTACATCCGTCAGGGTTCTGAGGAATGTCAGAAGTTTTGGACCTTCTTTGAACGCCTGCAGAGATTC
  CAGAATCTCAAGACCTCCAGGAAGGAGGAGAAAGACCCTGGACAGCCCAAGCACAGCATCCCAGCGCTGG
  CCGACCTACCTCGCACTTACGACCCACGTTACCGCATCAACCTCTCTGTTCTTGGCCCTGCCACGCGGGG
  CTCTCAGGGACTGGGCAGGCACTTGCCCGCGGAGAGAGTGGCTGAGTTCCGCCGAGCCCTGTTGCACTAC
  CTGGACTTTGGCCAGAAGCAGGCATTTGGGCGTCTGGCCAAGCTGCAGCGTGAGCGGGCAGCCCTCCCCA
  TCGCCCAGTATGGGAACCGCATCCTGCAGACGCTGAAGGAGCACCAGGTGGTGGTAGTGGCCGGTGACAC
  CGGCTGTGGCAAGTCCACTCAGGTGCCCCAGTACCTGCTGGCTGCTGGCTTCAGTCATGTGGCGTGCACC
  CAGCCCCGGCGGATCGCCTGCATCTCACTGGCCAAGCGTGTGGGCTTTGAGAGCCTCAGTCAGTATGGCT
  CACAGGTCGGCTACCAGATCCGCTTTGAGAGCACACGTTCGGCGGCCACCAAGATTGTATTCCTGACAGT
  GGGGCTGCTCCTGCGACAAATCCAGCGGGAACCCAGCCTGCCCCAGTATGAGGTCCTGATTGTGGATGAA
  GTCCATGAGCGGCATCTCCACAACGATTTCCTCCTGGGCGTCCTCCAGCGCCTGTTGCCCACGCGGCCTG
  ACCTCAAGGTCATCCTCATGTCGGCCACCATCAACATCTCGCTCTTCTCCAGCTATTTCAGCAATGCCCC
  TGTGGTACAGGTGCCTGGGAGGCTGTTCCCCATCACGGTTGTGTACCAGCCGCAGGAGGCGGAGCCGACC
  ACGTCCAAGTCAGAGAAGCTGGACCCGCGGCCTTTCCTGAGGGTGCTGGAGTCCATTGACCACAAGTACC
  CGCCTGAGGAGCGGGGTGACCTCCTCGTCTTCCTCAGCGGCATGGCGGAGATCAGCGCCGTGCTGGAGGC
  TGCCCAGACCTATGCCAGCCACACCCAGCGCTGGGTGGTACTGCCACTGCACAGCGCCCTGTCTGTGGCC
  GACCAGGACAAGGTATTTGATGTGGCACCCCCTGGAGTCCGGAAATGCATCCTCTCCACCAACATTGCTG
  AGACCTCAGTCACCATTGACGGGATCCGCTTCGTAGTAGATTCCGGAAAGGTGAAGGAGATGAGCTACGA
  TCCGCAGGCCAAGCTGCAACGGCTGCAGGAGTTCTGGATTAGTCAGGCCAGCGCAGAGCAGCGGAAGGGC
  CGGGCGGGCCGCACGGGCCCCGGAGTCTGCTTCCGCCTCTATGCCGAATCGGACTATGATGCCTTCGCCC
  CCTACCCCGTCCCAGAAATTCGGAGGGTGGCCCTGGACTCGTTGGTGCTGCAGATGAAGAGCATGAGTGT
  GGGGGACCCCCGAACCTTCCCCTTCATCGAGCCCCCACCACCAGCCAGCCTGGAAACCGCCATCCTCTAC
  CTCCGGGACCAGGGGGCCCTGGACAGCTCAGAGGCCCTCACACCCATTGGGTCCCTGCTAGCCCAGCTGC
  CTGTGGACGTTGTGATTGGGAAGATGCTGATCCTGGGCTCCATGTTCAGCCTGGTGGAGCCTGTGCTCAC
  CATCGCAGCCGCACTTAGCGTCCAGTCGCCCTTCACCCGCAGCGCCCAGAGCAGCCCAGAGTGCGCGGCA
  GCACGGCGGCCGCTGGAGAGCGACCAGGGTGACCCCTTCACGCTCTTCAACGTCTTCAACGCCTGGGTGC
  AGGTGAAATCTGAACGGAGCAGAAACTCTCGCAAGTGGTGCCGCCGCCGGGGCATAGAGGAGCATCGACT
  GTACGAAATGGCCAACCTTCGGCGCCAGTTCAAGGAGCTGTTGGAGGACCACGGGCTGCTGGCTGGGGCC
  CAGGCCGCGCAGGTAGGGGACAGCTACAGTCGGTTGCAGCAGCGCCGGGAGCGCCGGGCCCTGCACCAGC
  TGAAACGCCAGCACGAGGAGGGCGCGGGGCGCAGGCGCAAGGTGCTGCGGCTGCAGGAGGAGCAGGACGG
  CGGCTCCAGTGACGAGGACAGGGCTGGCCCAGCCCCCCCAGGGGCCAGTGATGGCGTGGACATCCAGGAT
  GTGAAGTTCAAGCTTCGGCATGACCTGGCGCAGCTGCAGGCCGCTGCCAGCTCAGCCCAGGACCTGAGCC
  GCGAGCAGCTGGCTCTGCTGAAGCTGGTGCTGGGCCGGGGCCTGTACCCACAGCTGGCCGTCCCCGACGC
  CTTCAACAGCAGCCGAAAGGACTCAGACCAGATTTTCCACACGCAGGCCAAGCAGGGCGCCGTGCTGCAC
  CCCACCTGCGTCTTCGCTGGCAGCCCCGAGGTGCTGCACGCACAGGAGCTGGAGGCCAGCAACTGCGACG
  GAAGCCGAGACGACAAGGACAAGATGAGCAGCAAACACCAGCTCCTCAGCTTCGTGTCCCTGCTGGAGAC
  CAACAAGCCGTACCTGGTGAACTGCGTCCGCATCCCTGCCCTCCAGTCCCTCCTGCTTTTTAGCCGGTCT
  TTGGACACCAATGGTGACTGCTCCCGCCTGGTGGCCGATGGCTGGCTGGAGCTGCAGCTAGCAGACAGTG
  AAAGTGCCATCCGACTCCTGGCGGCTTCCCTGCGGCTCCGTGCCCGCTGGGAAAGTGCCCTGGACCGGCA
  GCTGGCGCACCAGGCCCAGCAGCAGCTGGAGGAGGAGGAGGAGGATACGCCAGTCAGCCCCAAGGAGGTG
  GCCACCCTGAGCAAGGAACTCCTGCAATTCACGGCATCCAAGATTCCTTACAGCCTCCGGCGGCTCACAG
  GGCTAGAAGTCCAGAACATGTATGTGGGACCCCAGACCATCCCAGCCACCCCCCATCTTCCTGGCCTCTT
  TGGCAGCTCCACCCTGTCCCCCCACCCCACAAAGGGGGGCTACGCAGTCACTGACTTCCTCACCTACAAC
  TGCCTCACGAATGACACAGACCTGTACAGCGACTGTCTCCGAACCTTCTGGACCTGCCCCCACTGTGGCC
  TGCATGCGCCCCTCACGCCCCTGGAGCGCATCGCCCATGAGAACACCTGCCCCCAGGCCCCACAGGATGG
  GCCCCCAGGGGCTGAGGAAGCTGCCCTCGAAACCCTCCAGAAGACATCTGTCCTGCAGAGGCCCTACCAC
  TGCGAGGCCTGCGGGAAGGACTTCCTCTTTACACCCACAGAGGTGCTGCGCCACCGGAAGCAGCACGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213162 representing NM_014681
  Red=Cloning site Green=Tags(s)
  
  MPPPRTREGRDRRDHHRAPSEEEALEKWDWNCPETRRLLEDAFFREEDYIRQGSEECQKFWTFFERLQRF
  QNLKTSRKEEKDPGQPKHSIPALADLPRTYDPRYRINLSVLGPATRGSQGLGRHLPAERVAEFRRALLHY
  LDFGQKQAFGRLAKLQRERAALPIAQYGNRILQTLKEHQVVVVAGDTGCGKSTQVPQYLLAAGFSHVACT
  QPRRIACISLAKRVGFESLSQYGSQVGYQIRFESTRSAATKIVFLTVGLLLRQIQREPSLPQYEVLIVDE
  VHERHLHNDFLLGVLQRLLPTRPDLKVILMSATINISLFSSYFSNAPVVQVPGRLFPITVVYQPQEAEPT
  TSKSEKLDPRPFLRVLESIDHKYPPEERGDLLVFLSGMAEISAVLEAAQTYASHTQRWVVLPLHSALSVA
  DQDKVFDVAPPGVRKCILSTNIAETSVTIDGIRFVVDSGKVKEMSYDPQAKLQRLQEFWISQASAEQRKG
  RAGRTGPGVCFRLYAESDYDAFAPYPVPEIRRVALDSLVLQMKSMSVGDPRTFPFIEPPPPASLETAILY
  LRDQGALDSSEALTPIGSLLAQLPVDVVIGKMLILGSMFSLVEPVLTIAAALSVQSPFTRSAQSSPECAA
  ARRPLESDQGDPFTLFNVFNAWVQVKSERSRNSRKWCRRRGIEEHRLYEMANLRRQFKELLEDHGLLAGA
  QAAQVGDSYSRLQQRRERRALHQLKRQHEEGAGRRRKVLRLQEEQDGGSSDEDRAGPAPPGASDGVDIQD
  VKFKLRHDLAQLQAAASSAQDLSREQLALLKLVLGRGLYPQLAVPDAFNSSRKDSDQIFHTQAKQGAVLH
  PTCVFAGSPEVLHAQELEASNCDGSRDDKDKMSSKHQLLSFVSLLETNKPYLVNCVRIPALQSLLLFSRS
  LDTNGDCSRLVADGWLELQLADSESAIRLLAASLRLRARWESALDRQLAHQAQQQLEEEEEDTPVSPKEV
  ATLSKELLQFTASKIPYSLRRLTGLEVQNMYVGPQTIPATPHLPGLFGSSTLSPHPTKGGYAVTDFLTYN
  CLTNDTDLYSDCLRTFWTCPHCGLHAPLTPLERIAHENTCPQAPQDGPPGAEEAALETLQKTSVLQRPYH
  CEACGKDFLFTPTEVLRHRKQHV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014681
• ORF Size: 3429 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014681.6
• RefSeq Size: 4386 bp
• RefSeq ORF: 3432 bp
• Locus ID: 9704
• UniProt ID: Q14147
• Cytogenetics: 19q13.32
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. It is mapped to the glioma 19q tumor suppressor region and is a tumor suppressor candidate gene. [provided by RefSeq, Jul 2008]