SPCA2 (ATP2C2) (NM_014861) Human Tagged ORF Clone

CAT#: RG212990

ATP2C2 (tGFP-tagged) - Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2)

Product Images

Circular map for RG212990

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPCA2
• Synonyms: SPCA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212990 representing NM_014861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCATTTCCATTTGTTGAAATTTAAGACCAGAGTCATCTTTTCTGCTGTAATTATAATGGTCACTG
  GCTTGTGCCTTTTCCTCCTCTCTCTGCCCCATCTGCACGGGGTCTTTGAACAAGTCCCAGCACCTTGGTG
  GACAAGCCTGTGTCCCTGGCCCATCATGGAAGCCGCTGCCTTTCAGAGTGGGAGTCTGTACCCTGTTGCC
  TCATTCCTTGCTGCGCCCATGAGTGAGCTTGTGCCTGACCTCTCCTTCCAGGTGGACTTACACACTGGGC
  TGTCGGAGTTCTCGGTGACGCAGCGCCGGCTGGCCCATGGCTGGAATGAGTTTGTTGCTGACAACAGCGA
  ACCTGTGTGGAAGAAATACCTGGATCAGTTTAAGAACCCCCTGATCCTGCTGCTGCTGGGCTCTGCCCTG
  GTGAGTGTCCTCACCAAGGAGTATGAGGACGCCGTCAGCATCGCCACGGCAGTGCTTGTCGTGGTCACTG
  TCGCCTTCATCCAGGAGTACAGGTCGGAGAAATCTCTGGAAGAGCTGACCAAGCTGGTTCCTCCAGAATG
  TAACTGCCTAAGAGAAGGAAAACTCCAGCACCTGCTTGCTCGAGAACTGGTTCCTGGTGATGTCGTATCT
  CTCTCGATCGGAGACCGGATCCCTGCAGACATCCGACTCACTGAGGTCACGGACCTCTTGGTGGATGAAT
  CCAGTTTCACCGGGGAAGCCGAGCCATGTAGTAAAACAGACAGCCCCTTGACAGGCGGTGGGGACCTCAC
  CACCCTCAGCAACATCGTCTTCATGGGGACCCTGGTGCAGTATGGGAGGGGCCAGGGGGTCGTGATTGGA
  ACAGGGGAAAGCTCTCAGTTCGGAGAAGTGTTTAAGATGATGCAGGCTGAAGAGACACCTAAAACTCCTT
  TGCAGAAAAGCATGGACAGGCTAGGAAAGCAACTGACACTCTTCTCCTTTGGCATAATCGGTCTCATCAT
  GCTCATTGGCTGGTCGCAAGGGAAACAACTCCTGAGTATGTTCACGATCGGGGTCAGCCTGGCTGTGGCG
  GCTATTCCAGAGGGTCTGCCCATCGTCGTCATGGTGACGCTGGTCCTGGGAGTGCTGCGGATGGCCAAGA
  AGCGGGTCATCGTGAAGAAGTTACCCATCGTGGAGACTTTAGGTTGCTGCAGCGTTCTCTGTTCTGACAA
  GACGGGGACTCTGACTGCCAATGAAATGACAGTGACCCAGCTTGTAACGTCAGATGGGCTTCGTGCCGAG
  GTCAGCGGAGTTGGGTATGACGGTCAAGGGACTGTGTGTCTTCTACCATCCAAGGAAGTCATTAAGGAAT
  TTTCCAATGTCTCAGTGGGAAAGTTAGTGGAGGCGGGCTGTGTTGCCAACAATGCGGTCATCAGAAAGAA
  CGCCGTGATGGGGCAGCCCACCGAGGGTGCATTGATGGCCCTGGCGATGAAGATGGACTTAAGTGATATT
  AAAAATTCATATATAAGAAAAAAAGAGATTCCATTCAGTTCAGAGCAGAAGTGGATGGCGGTGAAATGCA
  GTCTGAAGACTGAGGATCAGGAAGACATTTACTTCATGAAAGGGGCCTTGGAAGAGGTGATCCGCTACTG
  CACCATGTACAACAACGGGGGCATCCCCCTGCCGCTGACGCCCCAGCAGAGGTCATTCTGCCTGCAGGAA
  GAGAAGAGGATGGGGTCGCTCGGTTTGCGGGTGCTGGCCCTGGCTTCTGGGCCCGAGCTGGGGCGGCTGA
  CGTTTCTAGGTCTTGTGGGCATCATTGACCCCCCGAGAGTTGGCGTGAAGGAAGCAGTCCAGGTTCTCTC
  CGAGTCTGGTGTGTCTGTGAAGATGATAACGGGGGATGCCCTGGAGACGGCCTTGGCCATAGGAAGAAAC
  ATCGGCCTGTGCAACGGGAAGCTGCAAGCCATGTCCGGGGAGGAGGTGGACAGCGTGGAGAAGGGCGAGC
  TGGCCGACCGCGTGGGGAAGGTGTCCGTGTTCTTCAGGACCAGCCCAAAGCACAAGCTCAAAATCATCAA
  GGCTCTGCAGGAGTCAGGGGCGATCGTGGCCATGACTGGGGATGGGGTGAACGACGCAGTGGCCCTGAAG
  TCTGCAGACATTGGGATCGCCATGGGGCAGACAGGGACGGACGTCAGCAAAGAGGCCGCCAACATGATCC
  TGGTGGATGATGACTTCTCAGCCATCATGAATGCAGTGGAGGAAGGCAAGGGTATTTTTTACAACATCAA
  AAACTTTGTCCGATTCCAGCTGAGCACGAGCATCTCCGCCCTGAGTCTCATCACTCTGTCCACCGTGTTC
  AACCTGCCCAGCCCCCTCAACGCCATGCAGATCCTATGGATCAACATCATCATGGATGGGCCACCGGCGC
  AGAGCTTGGGGGTAGAGCCCGTTGACAAAGACGCCTTCAGGCAGCCACCACGGAGTGTGCGGGACACCAT
  CCTCAGCAGAGCCCTCATCCTGAAGATCCTCATGTCCGCGGCCATCATCATCAGCGGGACCCTCTTTATC
  TTCTGGAAGGAGATGCCTGAAGACAGAGCAAGCACTCCCCGCACCACGACGATGACGTTCACTTGTTTTG
  TGTTTTTCGATCTCTTCAACGCCTTGACCTGCCGCTCTCAGACCAAGCTGATATTTGAGATCGGCTTTCT
  CAGGAACCACATGTTCCTCTACTCCGTCCTGGGGTCCATCCTGGGGCAGCTGGCGGTCATTTACATCCCC
  CCGCTGCAGAGGGTCTTCCAGACGGAGAACCTGGGAGCGCTTGATTTGCTGTTTTTAACTGGATTGGCCT
  CATCCGTCTTCATTTTGTCAGAGCTCCTCAAACTATGTGAAAAATACTGTTGCAGCCCCAAGAGAGTCCA
  GATGCACCCTGAAGATGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212990 representing NM_014861
  Red=Cloning site Green=Tags(s)
  
  MLHFHLLKFKTRVIFSAVIIMVTGLCLFLLSLPHLHGVFEQVPAPWWTSLCPWPIMEAAAFQSGSLYPVA
  SFLAAPMSELVPDLSFQVDLHTGLSEFSVTQRRLAHGWNEFVADNSEPVWKKYLDQFKNPLILLLLGSAL
  VSVLTKEYEDAVSIATAVLVVVTVAFIQEYRSEKSLEELTKLVPPECNCLREGKLQHLLARELVPGDVVS
  LSIGDRIPADIRLTEVTDLLVDESSFTGEAEPCSKTDSPLTGGGDLTTLSNIVFMGTLVQYGRGQGVVIG
  TGESSQFGEVFKMMQAEETPKTPLQKSMDRLGKQLTLFSFGIIGLIMLIGWSQGKQLLSMFTIGVSLAVA
  AIPEGLPIVVMVTLVLGVLRMAKKRVIVKKLPIVETLGCCSVLCSDKTGTLTANEMTVTQLVTSDGLRAE
  VSGVGYDGQGTVCLLPSKEVIKEFSNVSVGKLVEAGCVANNAVIRKNAVMGQPTEGALMALAMKMDLSDI
  KNSYIRKKEIPFSSEQKWMAVKCSLKTEDQEDIYFMKGALEEVIRYCTMYNNGGIPLPLTPQQRSFCLQE
  EKRMGSLGLRVLALASGPELGRLTFLGLVGIIDPPRVGVKEAVQVLSESGVSVKMITGDALETALAIGRN
  IGLCNGKLQAMSGEEVDSVEKGELADRVGKVSVFFRTSPKHKLKIIKALQESGAIVAMTGDGVNDAVALK
  SADIGIAMGQTGTDVSKEAANMILVDDDFSAIMNAVEEGKGIFYNIKNFVRFQLSTSISALSLITLSTVF
  NLPSPLNAMQILWINIIMDGPPAQSLGVEPVDKDAFRQPPRSVRDTILSRALILKILMSAAIIISGTLFI
  FWKEMPEDRASTPRTTTMTFTCFVFFDLFNALTCRSQTKLIFEIGFLRNHMFLYSVLGSILGQLAVIYIP
  PLQRVFQTENLGALDLLFLTGLASSVFILSELLKLCEKYCCSPKRVQMHPEDV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014861
• ORF Size: 2889 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014861.1, NP_055676.1
• RefSeq Size: 3600 bp
• RefSeq ORF: 2841 bp
• Locus ID: 9914
• UniProt ID: O75185
• Cytogenetics: 16q24.1
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.[UniProtKB/Swiss-Prot Function]