SPCA2 (ATP2C2) (NM_014861) Human Tagged ORF Clone

CAT#: RC212990

ATP2C2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2)

Product Images

Circular map for RC212990

• 

  Western blot validation of overexpression lysate (Cat# LY414989) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212990 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPCA2
• Synonyms: SPCA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212990 representing NM_014861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGAGGGACGCGTCTCCGAGTTCCTGAAGAAACTCGGCTTCTCGGGCGGGGGCCGCCAGTACCAGG
  CGCTGGAGAAGGACGAAGAGGAAGCCTTGATTGATGAACAGAGTGAGCTGAAAGCCATCGAGAAAGAGAA
  GAAGGTGACAGCCCTGCCCCCCAAGGAAGCGTGCAAATGCCAGAAAGAGGATTTGGCCAGAGCGTTTTGT
  GTGGACTTACACACTGGGCTGTCGGAGTTCTCGGTGACGCAGCGCCGGCTGGCCCATGGCTGGAATGAGT
  TTGTTGCTGACAACAGCGAACCTGTGTGGAAGAAATACCTGGATCAGTTTAAGAACCCCCTGATCCTGCT
  GCTGCTGGGCTCTGCCCTGGTGAGTGTCCTCACCAAGGAGTATGAGGACGCCGTCAGCATCGCCACGGCA
  GTGCTTGTCGTGGTCACTGTCGCCTTCATCCAGGAGTACAGGTCGGAGAAATCTCTGGAAGAGCTGACCA
  AGATGGTTCCTCCAGAATGTAACTGCCTAAGAGAAGGAAAACTCCAGCACCTGCTTGCTCGAGAACTGGT
  TCCTGGTGATGTCGTATCTCTCTCGATCGGAGACCGGATCCCTGCAGACATCCGACTCACTGAGGTCACG
  GACCTCTTGGTGGATGAATCCAGTTTCACCGGGGAAGCCGAGCCATGTAGTAAAACAGACAGCCCCTTGA
  CAGGCGGTGGGGACCTCACCACCCTCAGCAACATCGTCTTCATGGGGACCCTGGTGCAGTATGGGAGGGG
  CCAGGGGGTCGTGATTGGAACAGGGGAAAGCTCTCAGTTCGGAGAAGTGTTTAAGATGATGCAGGCTGAA
  GAGACACCTAAAACTCCTTTGCAGAAAAGCATGGACAGGCTAGGAAAGCAACTGACACTCTTCTCCTTTG
  GCATAATCGGTCTCATCATGCTCATTGGCTGGTCGCAAGGGAAACAACTCCTGAGTATGTTCACGATCGG
  GGTCAGCCTGGCTGTGGCGGCCATTCCAGAGGGTCTGCCCATCGTCGTCATGGTGACGCTGGTCCTGGGA
  GTGCTGCGGATGGCCAAGAAGCGGGTCATCGTGAAGAAGTTACCCATCGTGGAGACTTTAGGTTGCTGCA
  GCGTTCTCTGTTCTGACAAGACGGGGACTCTGACTGCCAATGAAATGACAGTGACCCAGCTTGTAACGTC
  AGATGGGCTTCGTGCCGAGGTCAGCGGAGTTGGGTATGACGGTCAAGGGACTGTGTGTCTTCTACCATCC
  AAGGAAGTCATTAAGGAATTTTCCAATGTCTCAGTGGGAAAGTTAGTGGAGGCGGGCTGTGTTGCCAACA
  ATGCGGTCATCAGAAAGAACGCCGTGATGGGGCAGCCCACCGAGGGTGCATTGATGGCCCTGGCGATGAA
  GATGGACTTAAGTGATATTAAAAATTCATATATAAGAAAAAAAGAGATTCCATTCAGTTCAGAGCAGAAG
  TGGATGGCGGTGAAATGCAGTCTGAAGACTGAGGATCAGGAAGACATTTACTTCATGAAAGGGGCCTTGG
  AAGAGGTGATCCGCTACTGCACCATGTACAACAACGGGGGCATCCCCCTGCCGCTGACGCCCCAGCAGAG
  GTCATTCTGCCTGCAGGAAGAGAAGAGGATGGGGTCGCTCGGTTTGCGGGTGCTGGCCCTGGCTTCTGGG
  CCCGAGCTGGGGCGGCTGACGTTTCTCGGTCTTGTGGGCATCATTGACCCCCCGAGAGTTGGCGTGAAGG
  AAGCAGTCCAGGTTCTCTCCGAGTCTGGTGTGTCTGTGAAGATGATAACGGGGGATGCCCTGGAGACGGC
  CTTGGCCATAGGAAGAAACATCGGCCTGTGCAACGGGAAGCTGCAAGCCATGTCCGGGGAGGAGGTGGAC
  AGCGTGGAGAAGGGCGAGCTGGCCGACCGCGTGGGGAAGGTGTCCGTGTTCTTCAGGACCAGCCCAAAGC
  ACAAGCTCAAAATCATCAAGGCTCTGCAGGAGTCAGGGGCGATCGTGGCCATGACTGGGGATGGGGTGAA
  CGACGCAGTGGCCCTGAAGTCTGCAGACATTGGGATCGCCATGGGGCAGACAGGGACGGACGTCAGCAAA
  GAGGCCGCCAACATGATCCTGGTGGATGATGACTTCTCAGCCATCATGAATGCAGTGGAGGAAGGCAAGG
  GTATTTTTTACAACATCAAAAACTTTGTCCGATTCCAGCTGAGCACGAGCATCTCCGCCCTGAGTCTCAT
  CACTCTGTCCACCGTGTTCAACCTGCCCAGCCCCCTCAACGCCATGCAGATCCTATGGATCAACATCATC
  ATGGATGGGCCACCGGCGCAGAGCTTGGGGGTAGAGCCCGTTGACAAAGACGCCTTCAGGCAGCCACCAC
  GGAGTGTGCGGGACACCATCCTCAGCAGAGCCCTCATCCTGAAGATCCTCATGTCCGCGGCCATCATCAT
  CAGCGGGACCCTCTTTATCTTCTGGAAGGAGATGCCTGAAGACAGAGCAAGCACTCCCCGCACCACGACG
  ATGACGTTCACTTGTTTTGTGTTTTTCGATCTCTTCAACGCCTTGACCTGCCGCTCTCAGACCAAGCTGA
  TATTTGAGATCGGCTTTCTCAGGAACCACATGTTCCTCTACTCCGTCCTGGGGTCCATCCTGGGGCAGCT
  GGCGGTCATTTACATCCCCCCGCTGCAGAGGGTCTTCCAGACGGAGAACCTGGGAGCGCTTGATTTGCTG
  TTTTTAACTGGATTGGCCTCATCCGTCTTCATTTTGTCAGAGCTCCTCAAACTATGTGAAAAATACTGTT
  GCAGCCCCAAGAGAGTCCAGATGCACCCTGAAGATGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212990 representing NM_014861
  Red=Cloning site Green=Tags(s)
  
  MVEGRVSEFLKKLGFSGGGRQYQALEKDEEEALIDEQSELKAIEKEKKVTALPPKEACKCQKEDLARAFC
  VDLHTGLSEFSVTQRRLAHGWNEFVADNSEPVWKKYLDQFKNPLILLLLGSALVSVLTKEYEDAVSIATA
  VLVVVTVAFIQEYRSEKSLEELTKMVPPECNCLREGKLQHLLARELVPGDVVSLSIGDRIPADIRLTEVT
  DLLVDESSFTGEAEPCSKTDSPLTGGGDLTTLSNIVFMGTLVQYGRGQGVVIGTGESSQFGEVFKMMQAE
  ETPKTPLQKSMDRLGKQLTLFSFGIIGLIMLIGWSQGKQLLSMFTIGVSLAVAAIPEGLPIVVMVTLVLG
  VLRMAKKRVIVKKLPIVETLGCCSVLCSDKTGTLTANEMTVTQLVTSDGLRAEVSGVGYDGQGTVCLLPS
  KEVIKEFSNVSVGKLVEAGCVANNAVIRKNAVMGQPTEGALMALAMKMDLSDIKNSYIRKKEIPFSSEQK
  WMAVKCSLKTEDQEDIYFMKGALEEVIRYCTMYNNGGIPLPLTPQQRSFCLQEEKRMGSLGLRVLALASG
  PELGRLTFLGLVGIIDPPRVGVKEAVQVLSESGVSVKMITGDALETALAIGRNIGLCNGKLQAMSGEEVD
  SVEKGELADRVGKVSVFFRTSPKHKLKIIKALQESGAIVAMTGDGVNDAVALKSADIGIAMGQTGTDVSK
  EAANMILVDDDFSAIMNAVEEGKGIFYNIKNFVRFQLSTSISALSLITLSTVFNLPSPLNAMQILWINII
  MDGPPAQSLGVEPVDKDAFRQPPRSVRDTILSRALILKILMSAAIIISGTLFIFWKEMPEDRASTPRTTT
  MTFTCFVFFDLFNALTCRSQTKLIFEIGFLRNHMFLYSVLGSILGQLAVIYIPPLQRVFQTENLGALDLL
  FLTGLASSVFILSELLKLCEKYCCSPKRVQMHPEDV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014861
• ORF Size: 2838 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014861.2
• RefSeq Size: 3600 bp
• RefSeq ORF: 2841 bp
• Locus ID: 9914
• UniProt ID: O75185
• Cytogenetics: 16q24.1
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• MW: 103 kDa
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.[UniProtKB/Swiss-Prot Function]