PEPP2 (PLEKHA5) (NM_019012) Human Tagged ORF Clone

CAT#: RG212944

PLEKHA5 (tGFP-tagged) - Human pleckstrin homology domain containing, family A member 5 (PLEKHA5), transcript variant 1

Product Images

Circular map for RG212944

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PEPP2
• Synonyms: PEPP-2; PEPP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212944 representing NM_019012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGATCTAAACCTGGAGTGGATCTCCCTGCCCCGGTCCTGGACTTACGGGATCACCAGGGGCG
  GCCGAGTCTTCTTCATCAACGAGGAGGCCAAGAGCACCACCTGGCTGCACCCCGTCACCGGCGAGGCGGT
  GGTCACCGGACACCGGCGGCAGAGCACAGATTTGCCTACTGGCTGGGAAGAAGCATATACTTTTGAAGGT
  GCAAGATACTATATAAACCATAATGAAAGGAAAGTGACCTGCAAACATCCAGTCACAGGACAACCATCAC
  AGGACAATTGTATTTTTGTAGTGAATGAACAGACTGTTGCAACCATGACATCTGAAGAAAAGAAGGAACG
  GCCAATAAGTATGATAAATGAAGCTTCTAACTATAACGTGACTTCAGATTATGCAGTGCATCCAATGAGC
  CCTGTAGGCAGAACTTCACGAGCTTCAAAAAAAGTTCATAATTTTGGAAAGAGGTCAAATTCAATTAAAA
  GGAATCCTAATGCACCGGTTGTCAGACGAGGTTGGCTTTATAAACAGGACAGTACTGGCATGAAATTGTG
  GAAGAAACGCTGGTTTGTGCTTTCTGACCTTTGCCTCTTTTATTATAGAGATGAGAAAGAAGAGGGTATC
  CTGGGAAGCATACTGTTACCTAGTTTTCAGATAGCTTTGCTTACCTCTGAAGATCACATTAATCGCAAAT
  ATGCTTTTAAGGCAGCCCATCCAAACATGCGGACCTATTATTTCTGCACTGATACAGGAAAGGAAATGGA
  GTTGTGGATGAAAGCCATGTTAGATGCTGCCCTAGTACAGACAGAACCTGTGAAAAGAGTGGACAAGATT
  ACATCTGAAAATGCACCAACTAAAGAAACCAATAACATTCCCAACCATAGAGTGCTAATTAAACCAGAGA
  TCCAAAACAATCAAAAAAACAAGGAAATGAGCAAAATTGAAGAAAAAAAGGCATTAGAAGCTGAAAAATA
  TGGATTTCAGAAGGATGGTCAAGATAGACCCTTAACAAAAATTAATAGTGTAAAGCTGAATTCTCTGCCA
  TCTGAATATGAGAGTGGGTCAGCATGCCCTGCTCAGACTGTGCACTACAGACCAATCAACTTGAGCAGTT
  CAGAGAACAAAATAGTCAATGTTAGCCTGGCAGATCTTAGAGGTGGAAATCGCCCCAATACAGGGCCCTT
  ATACACAGAGGCCGATCGAGTCATACAGAGAACAAATTCAATGCAGCAGTTGGAACAGTGGATTAAAATC
  CAGAAGGGGAGGGGTCATGAAGAAGAAACCAGGGGAGTAATTTCTTACCAAACATTACCAAGAAATATGC
  CAAGTCACAGAGCCCAGATTATGGCCCGCTACCCTGAAGGTTATAGAACACTCCCAAGAAACAGCAAGAC
  AAGGCCTGAAAGTATCTGCAGTGTAACCCCTTCCACTCATGACAAGACATTAGGACCCGGAGCGGAGGAG
  AAACGGAGGTCCATGAGAGATGACACAATGTGGCAGCTCTACGAATGGCAGCAGCGTCAGTTTTATAACA
  AACAGAGCACCCTCCCTCGACACAGTACTTTGAGTAGTCCCAAAACCATGGTAAATATTTCTGACCAGAC
  AATGCACTCTATTCCCACATCACCTTCCCACGGGTCAATAGCTGCTTATCAGGGATACTCCCCTCAACGA
  ACTTACAGATCGGAAGTGTCTTCACCAATTCAGAGAGGAGATGTGACAATAGACCGCAGACACAGGGCCC
  ATCACCCTAAGCATGTCTATGTGCCTGACAGAAGGTCAGTGCCAGCTGGCCTGACTTTACAGTCTGTTAG
  TCCCCAGAGCCTCCAAGGGAAAACGCTGTCACAAGATGAAGGTAGAGGCACATTATACAAATACAGACCT
  GAAGAAGTAGATATTGATGCCAAGTTAAGCCGATTATGTGAACAAGATAAAGTGGTGCATGCTCTGGAAG
  AGAAACTTCAGCAACTCCACAAGGAGAAATACACGCTTGAGCAAGCTTTGCTATCAGCCAGCCAAGAGAT
  AGAAATGCATGCAGATAACCCAGCAGCCATTCAGACAGTGGTGTTACAAAGGGATGATTTACAAAATGGA
  CTGCTTAGTACGTGTCGAGAACTTTCTCGAGCCACTGCCGAATTGGAACGAGCATGGAGAGAATATGATA
  AGTTAGAATACGATGTAACTGTTACCAGGAACCAGATGCAAGAGCAGCTGGATCACCTTGGTGAAGTTCA
  GACGGAATCAGCAGGAATTCAGCGTGCACAGATTCAGAAAGAACTTTGGCGAATTCAGGATGTCATGGAA
  GGGCTGAGTAAACATAAGCAGCAAAGAGGTACTACAGAAATAGGTATGATAGGATCAAAGCCTTTCTCAA
  CAGTTAAGTACAAAAATGAGGGTCCAGATTATAGACTCTACAAGAGTGAACCAGAGTTAACAACAGTGGC
  AGAAGTTGATGAATCTAATGGAGAAGAAAAATCAGAACCTGTTTCAGAGATAGAAACTTCAGTTGTTAAA
  GGTTCCCACTTTCCTGTTGGAGTAGTCCCTCCAAGAGCAAAATCACCAACACCCGAATCTTCGACAATAG
  CTTCCTATGTAACCTTGAGGAAAACTAAGAAGATGATGGATCTAAGAACGGAAAGACCAAGAAGTGCAGT
  GGAACAGCTCTGTTTGGCTGAAAGTACTCGACCAAGGATGACTGTGGAAGAGCAAATGGAAAGAATAAGA
  AGACATCAACAAGCGTGCCTGAGGGAGAAGAAAAAAGGGTTAAATGTTATCGGTGCTTCAGACCAGTCAC
  CCTTACAAAGCCCTTCAAATTTAAGGGATAATCCATTTAGGACTACTCAGACTCGAAGGAGGGATGATAA
  GGAACTGGACACTGCCATTAGAGAAAATGATGTAAAGCCAGACCATGAAACTCCTGCAACAGAAATTGTT
  CAACTAAAAGAAACCGAACCCCAAAATGTGGACTTCAGCAAAGAGTTAAAAAAAACTGAAAACATTTCAT
  ATGAAATGCTTTTTGAACCTGAGCCAAATGGAGTAAATTCTGTGGAAATGATGGATAAAGAAAGAAACAA
  AGACAAAATGCCTGAGGATGTTACATTCAGCCCTCAAGATGAAACACAGACCGCAAATCATAAACCAGAA
  GAGCATCCTGAAGAAAATACAAAGAACAGTGTTGACGAACAGGAAGAAACTGTTATTTCTTACGAATCAA
  CTCCTGAGGTTTCTAGAGGAAATCAAACAATGGCAGTGAAAAGTCTGTCCCCATCTCCTGAGTCCTCGGC
  ATCGCCAGTTCCATCCACTCAGCCGCAGCTCACAGAAGGATCACATTTCATGTGTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212944 representing NM_019012
  Red=Cloning site Green=Tags(s)
  
  MAADLNLEWISLPRSWTYGITRGGRVFFINEEAKSTTWLHPVTGEAVVTGHRRQSTDLPTGWEEAYTFEG
  ARYYINHNERKVTCKHPVTGQPSQDNCIFVVNEQTVATMTSEEKKERPISMINEASNYNVTSDYAVHPMS
  PVGRTSRASKKVHNFGKRSNSIKRNPNAPVVRRGWLYKQDSTGMKLWKKRWFVLSDLCLFYYRDEKEEGI
  LGSILLPSFQIALLTSEDHINRKYAFKAAHPNMRTYYFCTDTGKEMELWMKAMLDAALVQTEPVKRVDKI
  TSENAPTKETNNIPNHRVLIKPEIQNNQKNKEMSKIEEKKALEAEKYGFQKDGQDRPLTKINSVKLNSLP
  SEYESGSACPAQTVHYRPINLSSSENKIVNVSLADLRGGNRPNTGPLYTEADRVIQRTNSMQQLEQWIKI
  QKGRGHEEETRGVISYQTLPRNMPSHRAQIMARYPEGYRTLPRNSKTRPESICSVTPSTHDKTLGPGAEE
  KRRSMRDDTMWQLYEWQQRQFYNKQSTLPRHSTLSSPKTMVNISDQTMHSIPTSPSHGSIAAYQGYSPQR
  TYRSEVSSPIQRGDVTIDRRHRAHHPKHVYVPDRRSVPAGLTLQSVSPQSLQGKTLSQDEGRGTLYKYRP
  EEVDIDAKLSRLCEQDKVVHALEEKLQQLHKEKYTLEQALLSASQEIEMHADNPAAIQTVVLQRDDLQNG
  LLSTCRELSRATAELERAWREYDKLEYDVTVTRNQMQEQLDHLGEVQTESAGIQRAQIQKELWRIQDVME
  GLSKHKQQRGTTEIGMIGSKPFSTVKYKNEGPDYRLYKSEPELTTVAEVDESNGEEKSEPVSEIETSVVK
  GSHFPVGVVPPRAKSPTPESSTIASYVTLRKTKKMMDLRTERPRSAVEQLCLAESTRPRMTVEEQMERIR
  RHQQACLREKKKGLNVIGASDQSPLQSPSNLRDNPFRTTQTRRRDDKELDTAIRENDVKPDHETPATEIV
  QLKETEPQNVDFSKELKKTENISYEMLFEPEPNGVNSVEMMDKERNKDKMPEDVTFSPQDETQTANHKPE
  EHPEENTKNSVDEQEETVISYESTPEVSRGNQTMAVKSLSPSPESSASPVPSTQPQLTEGSHFMCV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019012
• ORF Size: 3348 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019012.2, NP_061885.2
• RefSeq Size: 4253 bp
• RefSeq ORF: 3351 bp
• Locus ID: 54477
• UniProt ID: Q9HAU0
• Cytogenetics: 12p12.3
• Domains: WW, PH