OSGIN1 (NM_182981) Human Tagged ORF Clone

CAT#: RG212870

OSGIN1 (tGFP-tagged) - Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 3

Product Images

Circular map for RG212870

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OSGIN1
• Synonyms: BDGI; OKL38
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212870 representing NM_182981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTCCAGAAAGGACCACCTCGGCGCCAGCAGCTCAGAGCCCCTCCCGGTCATCATTGTGGGTA
  ACGGCCCCTCTGGTATCTGCCTGTCCTACCTGCTCTCCGGCTACACACCCTACACGAAGCCAGATGCCAT
  CCACCCACACCCCCTGCTGCAGAGGAAGCTCACCGAGGCCCCGGGGGTCTCCATCCTGGACCAGGACCTG
  GACTACCTGTCCGAAGGCCTCGAAGGCCGATCCCAAAGCCCCGTGGCCCTGCTCTTTGATGCCCTTCTAC
  GCCCAGACACAGACTTTGGGGGAAACATGAAGTCGGTCCTCACCTGGAAGCACCGGAAGGAGCACGCCAT
  CCCCCACGTGGTTCTGGGCCGGAACCTCCCCGGGGGAGCCTGGCACTCCATCGAAGGCTCCATGGTGATC
  CTGAGCCAAGGCCAGTGGATGGGGCTCCCGGACCTGGAGGTCAAGGACTGGATGCAGAAGAAGCGAAGAG
  GTCTTCGCAACAGCCGGGCCACTGCCGGGGACATCGCCCACTACTACAGGGACTACGTGGTCAAGAAGGG
  TCTGGGGCATAACTTTGTGTCCGGTGCTGTAGTCACAGCCGTGGAGTGGGGGACCCCCGATCCCAGCAGC
  TGTGGGGCCCAGGACTCCAGCCCCCTCTTCCAGGTGAGCGGCTTCCTGACCAGGAACCAGGCCCAGCAGC
  CCTTCTCGCTGTGGGCCCGCAACGTGGTCCTCGCCACAGGCACGTTCGACAGCCCGGCCCGGCTGGGCAT
  CCCCGGGGAGGCCCTGCCCTTCATCCACCATGAGCTGTCTGCCCTGGAGGCCGCCACAAGGGTGGGTGCG
  GTGACCCCGGCCTCAGACCCTGTCCTCATCATTGGCGCGGGGCTGTCAGCGGCCGACGCGGTCCTCTACG
  CCCGCCACTACAACATCCCGGTGATCCATGCCTTCCGCCGGGCCGTGGACGACCCTGGCCTGGTGTTCAA
  CCAGCTGCCCAAGATGCTGTACCCCGAGTACCACAAGGTGCACCAGATGATGCGGGAGCAGTCCATCCTG
  TCGCCCAGCCCCTATGAGGGTTACCGCAGCCTCCCCAGGCACCAGCTGCTGTGCTTCAAGGAAGACTGCC
  AGGCCGTGTTCCAGGACCTCGAGGGTGTCGAGAAGGTGTTTGGGGTCTCCCTGGTGCTGGTCCTCATCGG
  CTCCCACCCCGACCTCTCCTTCCTGCCTGGGGCAGGGGCTGACTTTGCAGTGGATCCTGACCAGCCGCTG
  AGCGCCAAGAGGAACCCCATTGACGTGGACCCCTTCACCTACCAGAGCACCCGCCAGGAGGGCCTGTACG
  CCATGGGGCCGCTGGCCGGGGACAACTTCGTGAGGTTTGTGCAGGGGGGCGCCTTGGCTGTGGCCAGCTC
  CCTGCTAAGGAAGGAGACCAGGAAGCCACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212870 representing NM_182981
  Red=Cloning site Green=Tags(s)
  
  MSSSRKDHLGASSSEPLPVIIVGNGPSGICLSYLLSGYTPYTKPDAIHPHPLLQRKLTEAPGVSILDQDL
  DYLSEGLEGRSQSPVALLFDALLRPDTDFGGNMKSVLTWKHRKEHAIPHVVLGRNLPGGAWHSIEGSMVI
  LSQGQWMGLPDLEVKDWMQKKRRGLRNSRATAGDIAHYYRDYVVKKGLGHNFVSGAVVTAVEWGTPDPSS
  CGAQDSSPLFQVSGFLTRNQAQQPFSLWARNVVLATGTFDSPARLGIPGEALPFIHHELSALEAATRVGA
  VTPASDPVLIIGAGLSAADAVLYARHYNIPVIHAFRRAVDDPGLVFNQLPKMLYPEYHKVHQMMREQSIL
  SPSPYEGYRSLPRHQLLCFKEDCQAVFQDLEGVEKVFGVSLVLVLIGSHPDLSFLPGAGADFAVDPDQPL
  SAKRNPIDVDPFTYQSTRQEGLYAMGPLAGDNFVRFVQGGALAVASSLLRKETRKPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182981
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182981.3
• RefSeq Size: 1930 bp
• RefSeq ORF: 1434 bp
• Locus ID: 29948
• UniProt ID: Q9UJX0
• Cytogenetics: 16q23.3
• Gene Summary: This gene encodes an oxidative stress response protein that regulates cell death. Expression of the gene is regulated by p53 and is induced by DNA damage. The protein regulates apoptosis by inducing cytochrome c release from mitochondria. It also appears to be a key regulator of both inflammatory and anti-inflammatory molecules. The loss of this protein correlates with uncontrolled cell growth and tumor formation. Naturally occurring read-through transcription exists between this gene and the neighboring upstream malonyl-CoA decarboxylase (MLYCD) gene, but the read-through transcripts are unlikely to produce a protein product. [provided by RefSeq, Aug 2011]