SPC24 (NM_182513) Human Tagged ORF Clone

CAT#: RG211241

SPC24 (tGFP-tagged) - Human SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (SPC24)

Product Images

Circular map for RG211241

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPC24
• Synonyms: SPBC24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211241 representing NM_182513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCTTCCGCGACATAGAGGAGGTGAGCCAGGGGCTGCTCAGCCTGCTGGGCGCCAACCGCGCGG
  AGGCGCAGCAGCGACGGCTGCTGGGGCGCCACGAGCAGGTGGTGGAGCGGCTGCTGGAAACGCAAGACGG
  TGCCGAGAAGCAGCTGCGAGAGATCCTCACCATGGAGAAGGAAGTGGCCCAGAGCCTTCTCAATGCGAAG
  GAGCAGGTGCACCAGGGAGGCGTGGAGCTGCAGCAGCTGGAAGCTGGGCTTCAGGAGGCTGGGGAGGAGG
  ACACCCGTCTGAAGGCCAGCCTCCTTCAGCTCACCAGAGAGCTGGAAGAGCTCAAGGAGATTGAGGCGGA
  TCTGGAGCGACAGGAGAAGGAGGTCGACGAGGACACGACAGTCACAATCCCCTCGGCCGTGTACGTGGCT
  CAACTTTACCACCAAGTTAGTAAAATTGAGTGGGATTATGAGTGTGAGCCAGGGATGGTCAAAGGCATCC
  ATCATGGCCCCAGTGTGGCCCAGCCCATCCACCTGGACAGCACCCAGCTCTCCAGGAAATTCATCAGCGA
  CTACCTCTGGAGTCTGGTGGACACCGAGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211241 representing NM_182513
  Red=Cloning site Green=Tags(s)
  
  MAAFRDIEEVSQGLLSLLGANRAEAQQRRLLGRHEQVVERLLETQDGAEKQLREILTMEKEVAQSLLNAK
  EQVHQGGVELQQLEAGLQEAGEEDTRLKASLLQLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVA
  QLYHQVSKIEWDYECEPGMVKGIHHGPSVAQPIHLDSTQLSRKFISDYLWSLVDTEW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182513
• ORF Size: 591 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182513.3, NP_872319.1
• RefSeq Size: 2294 bp
• RefSeq ORF: 594 bp
• Locus ID: 147841
• UniProt ID: Q8NBT2
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• Gene Summary: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).[UniProtKB/Swiss-Prot Function]