NECAP1 (NM_015509) Human Tagged ORF Clone

CAT#: RG211100

NECAP1 (tGFP-tagged) - Human NECAP endocytosis associated 1 (NECAP1), transcript variant 1

Product Images

Circular map for RG211100

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NECAP1
• Synonyms: DEE21; EIEE21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211100 representing NM_015509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGAGTTGGAGTACGAGTCTGTGCTGTGTGTGAAGCCAGACGTCAGCGTCTACCGGATTCCGC
  CCCGGGCCTCCAACCGCGGTTACAGGGCCTCTGACTGGAAATTAGACCAGCCTGATTGGACTGGTCGCCT
  CCGAATCACTTCAAAAGGGAAGACTGCCTATATCAAACTCGAGGATAAAGTTTCAGGGGAGCTCTTTGCT
  CAGGCACCAGTAGAACAATATCCTGGTATTGCTGTGGAGACGGTGACAGATTCTAGCCGCTACTTTGTAA
  TCCGGATCCAGGATGGTACTGGGCGCAGTGCTTTCATTGGCATTGGCTTCACAGATCGGGGAGATGCCTT
  CGACTTTAATGTCTCCTTGCAGGATCACTTCAAGTGGGTAAAGCAGGAATCTGAGATTTCCAAGGAATCT
  CAAGAAATGGATGCTCGTCCTAAGTTGGATCTGGGCTTCAAGGAAGGACAAACCATCAAGTTGTGTATCG
  GGAACATTACAAACAAGAAAGGAGGTGCTTCTAAGCCCAGGACTGCAAGGGGTGGGGGTCTGAGCTTACT
  CCCACCCCCGCCAGGAGGCAAAGTCACTATTCCCCCACCATCCTCCTCAGTTGCCATCAGCAATCATGTC
  ACCCCACCACCCATTCCGAAATCTAACCATGGAGGCAGTGATGCAGATATCCTTTTAGATTTGGATTCTC
  CTGCTCCTGTCACGACACCAGCACCAACTCCAGTTTCTGTAAGCAATGACTTGTGGGGAGACTTCAGCAC
  TGCCTCCAGCTCTGTTCCAAACCAGGCACCACAGCCATCCAACTGGGTCCAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211100 representing NM_015509
  Red=Cloning site Green=Tags(s)
  
  MATELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKTAYIKLEDKVSGELFA
  QAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKES
  QEMDARPKLDLGFKEGQTIKLCIGNITNKKGGASKPRTARGGGLSLLPPPPGGKVTIPPPSSSVAISNHV
  TPPPIPKSNHGGSDADILLDLDSPAPVTTPAPTPVSVSNDLWGDFSTASSSVPNQAPQPSNWVQF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015509
• ORF Size: 825 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015509.4
• RefSeq Size: 2563 bp
• RefSeq ORF: 828 bp
• Locus ID: 25977
• UniProt ID: Q8NC96
• Cytogenetics: 12p13.31
• Gene Summary: This gene encodes a protein containing two characteristic WXXF motifs. The encoded protein localizes to clathrin-coated vesicles, where it binds components of the adapter protein complexes and aids in endocytosis. Loss of function of this gene results in early infantile epileptic encephalopathy-21. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]