SALF (STON1-GTF2A1L) (NM_172311) Human Tagged ORF Clone

CAT#: RG211018

STON1 (tGFP-tagged) - Human STON1-GTF2A1L readthrough (STON1-GTF2A1L), transcript variant 1

Product Images

Circular map for RG211018

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SALF
• Synonyms: ALF; GTF2A1L; GTF2A1LF; SALF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211018 representing NM_172311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCCACAAATCCAGGCAAATGGGTCACCTTTGATGATGATCCTGCTGTTCAATCTTCTCAAAAGT
  CAAAGAATTTTCCTCTGGAGAATCAAGGTGTCTGTAGACCAAATGGACTGAAGCTGAACCTTCCTGGCCT
  CAGGGAATTTCCCAGTGGATCTTCCTCCACCAGCAGCACTCCTCTCTCCTCCCCCATTGTAGATTTTTAT
  TTCAGTCCAGGACCTCCAAGTAACTCTCCTCTTTCTACACCTACCAAAGACTTCCCAGGTTTTCCTGGCA
  TCCCCAAAGCAGGGACTCATGTGCTTTATCCTATTCCAGAATCATCTTCAGACAGCCCACTCGCAATATC
  AGGAGGAGAATCTTCCTTACTGCCTACCAGACCAACATGTTTATCCCATGCCTTGTTACCCAGTGACCAC
  TCATGTACACATCCAACTCCCAAAGTAGGTCTTCCAGATGAAGTTAATCCTCAACAGGCTGAAAGCCTAG
  GATTCCAAAGTGATGATCTCCCCCAGTTTCAGTATTTTCGAGAGGACTGTGCTTTTTCAAGTCCATTTTG
  GAAAGATGAAGGCAGTGATTCCCATTTCACCCTTGACCCACCAGGAAGCAAAAAGATGTTCTCATCAAGA
  AACAAGGAGATGCCTATTGACCAAAAAAGCCTAAATAAGTGTTCACTCAACTATATCTGTGAGAAGCTTG
  AACATCTCCAGTCAGCTGAGAACCAAGACTCACTTAGAAGTTTGTCTATGCACTGTCTATGTGCTGAAGA
  AAATGCCTCTTCCTTTGTCCCCCACACACTCTTCAGGAGTCAGCCAAAATCTGGATGGTCTTTCATGCTG
  AGAATTCCTGAGAAGAAGAATATGATGTCTTCCCGGCAATGGGGACCAATTTTTCTGAAAGTTTTGCCTG
  GAGGAATTTTGCAGATGTATTATGAACAGGGATTAGAAAAACCATTTAAAGAGATACAGCTTGATCCATA
  TTGTAGGCTTTCTGAACCCAAGGTTGAGAACTTCAGTGTAGCAGGAAAAATCCACACTGTGAAGATTGAA
  CATGTGTCTTACACAGAAAAAAGGAAATACCATTCTAAGACAGAAGTAGTTCATGAACCTGACATAGAGC
  AGATGCTGAAGTTGGGGTCCACATCGTACCATGACTTCCTTGACTTTCTGACTACTGTGGAGGAGGAGCT
  GATGAAGTTGCCAGCTGTTTCAAAACCAAAAAAGAACTACGAGGAGCAAGAAATTTCCTTGGAAATTGTG
  GACAACTTTTGGGGTAAAGTCACAAAAGAAGGAAAATTTGTTGAAAGTGCTGTGATAACTCAAATTTATT
  GCCTCTGCTTTGTGAATGGGAACCTGGAATGCTTTTTAACCTTGAATGACCTTGAGTTGCCGAAGCGAGA
  TGAATCCTATTATGAGAAGGACTCAGAAAAAAAGGGGATTGATATTCTTGACTACCATTTTCATAAGTGT
  GTGAATGTACAAGAATTTGAGCAATCAAGAATCATTAAGTTTGTACCTCTGGATGCCTGCCGGTTTGAGC
  TGATGCGTTTCAAGACTTTGTATAATGGGGATAATCTTCCCTTTTCCTTGAAGTCTGTAGTGGTTGTCCA
  GGGAGCATACGTGGAACTTCAGGCTTTTGTCAACATGGCCTCATTGGCGCAGAGGTCATCCTATGCTGGT
  TCCTTAAGGTCCTGTGACAATATAAGGATACACTTTCCTGTCCCATCGCAGTGGATCAAGGCCCTTTGGA
  CCATGAACCTCCAGAGGCAGAAGTCTCTGAAAGCTAAAATGAACCGCCGAGCATGTCTGGGGAGTTTACA
  TGAACTTGAATCTGAACCTGTCATTCAAGTCACTGTGGGGTCAGCAAAATATGAGAGTGCCTACCAGGCA
  GTGGTATGGAAGATAGATCGGCTTCCAGACAAAAATTCAAGTCTAGATCATCCCCATTGTCTGTCATACA
  AATTAGAGCTTGGATCAGACCAAGAAATTCCCTCTGATTGGTATCCATTTGCTACTGTTCAGTTTTCCGT
  GCCTGACACCTGTGCCTCAAGGACAGAGGTCAGGTCTCTGGGAGTGGAGAGTGATGTCCAGCCACAGAAA
  CATGTTCAGCAGCGAGCTTGCTACAACATCCAGCCTAAACTCTACAGATCTGTAATTGAAGATGTAATTG
  AAGGAGTTCGGAATCTATTTGCTGAAGAAGGTATAGAGGAACAAGTTTTAAAAGACTTGAAGCAGCTCTG
  GGAAACCAAGGTTTTGCAGTCTAAAGCAACAGAAGACTTCTTCAGAAATAGCATCCAATCACCTCTGTTT
  ACTCTTCAGTTGCCGCACAGCTTGCACCAAACATTGCAATCGTCAACAGCATCATTAGTTATTCCTGCTG
  GTAGAACTCTTCCAAGTTTTACCACAGCAGAACTGGGCACTTCAAACTCCAGTGCAAACTTTACTTTTCC
  TGGTTATCCCATTCATGTACCAGCAGGTGTGACACTACAGACTGTATCTGGTCACCTTTATAAAGTCAAT
  GTACCAATTATGGTGACAGAGACTTCTGGAAGAGCAGGTATTCTTCAGCATCCAATTCAGCAAGTATTTC
  AACAGCTTGGCCAGCCTTCAGTAATACAAACTAGTGTTCCACAATTGAATCCATGGTCTCTTCAAGCAAC
  TACTGAAAAATCACAGAGAATTGAAACCGTGCTACAGCAACCCGCAATTCTACCTTCTGGGCCAGTAGAT
  AGGAAACACTTAGAAAATGCCACCAGTGATATACTTGTATCTCCTGGAAATGAGCATAAAATCGTGCCTG
  AAGCTTTGTTGTGTCATCAGGAAAGTTCTCACTATATCAGTCTTCCAGGTGTTGTATTTTCTCCACAGGT
  CTCTCAAACAAATTCTAATGTGGAGTCAGTGCTCAGTGGTTCAGCTAGCATGGCTCAAAATCTGCATGAT
  GAGTCCCTCTCCACAAGCCCTCATGGGGCTCTCCACCAGCACGTGACTGATATTCAGCTTCATATTCTTA
  AAAATAGGATGTATGGATGTGATTCTGTAAAGCAACCAAGAAATATAGAGGAACCCAGCAACATACCTGT
  ATCAGAGAAGGATTCTAATTCTCAGGTGGATTTAAGCATTCGGGTTACTGATGATGATATTGGTGAAATA
  ATTCAAGTAGATGGAAGCGGTGATACATCTTCCAATGAAGAAATAGGAAGTACAAGAGATGCAGATGAGA
  ATGAATTTCTAGGGAATATTGACGGGGGAGATCTGAAGGTACCTGAAGAAGAAGCTGACAGTATTTCAAA
  TGAGGATTCAGCCACAAACAGTAGTGATAATGAAGACCCTCAAGTAAACATTGTAGAAGAGGACCCTTTA
  AATTCTGGAGATGATGTTAGTGAACAGGATGTGCCAGACCTGTTTGACACGGATAATGTAATTGTCTGTC
  AGTATGATAAGATTCATCGAAGCAAGAACAAATGGAAATTCTATTTGAAAGATGGTGTTATGTGTTTTGG
  AGGGAGAGACTATGTATTTGCAAAAGCCATTGGTGATGCAGAGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211018 representing NM_172311
  Red=Cloning site Green=Tags(s)
  
  MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSSTPLSSPIVDFY
  FSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGESSLLPTRPTCLSHALLPSDH
  SCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDCAFSSPFWKDEGSDSHFTLDPPGSKKMFSSR
  NKEMPIDQKSLNKCSLNYICEKLEHLQSAENQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFML
  RIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIE
  HVSYTEKRKYHSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIV
  DNFWGKVTKEGKFVESAVITQIYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGIDILDYHFHKC
  VNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAYVELQAFVNMASLAQRSSYAG
  SLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLHELESEPVIQVTVGSAKYESAYQA
  VVWKIDRLPDKNSSLDHPHCLSYKLELGSDQEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQK
  HVQQRACYNIQPKLYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWETKVLQSKATEDFFRNSIQSPLF
  TLQLPHSLHQTLQSSTASLVIPAGRTLPSFTTAELGTSNSSANFTFPGYPIHVPAGVTLQTVSGHLYKVN
  VPIMVTETSGRAGILQHPIQQVFQQLGQPSVIQTSVPQLNPWSLQATTEKSQRIETVLQQPAILPSGPVD
  RKHLENATSDILVSPGNEHKIVPEALLCHQESSHYISLPGVVFSPQVSQTNSNVESVLSGSASMAQNLHD
  ESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEI
  IQVDGSGDTSSNEEIGSTRDADENEFLGNIDGGDLKVPEEEADSISNEDSATNSSDNEDPQVNIVEEDPL
  NSGDDVSEQDVPDLFDTDNVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIGDAEW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172311
• ORF Size: 3546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172311.1, NP_758515.1
• RefSeq Size: 3824 bp
• RefSeq ORF: 3549 bp
• Locus ID: 286749
• Cytogenetics: 2p16.3
• Protein Families: Transcription Factors
• Gene Summary: STON1-GTF2A1L mRNAs are infrequent but naturally occurring read-through products of the neighboring STON1 and GTF2A1L genes. These transcripts encode fusion proteins composed of the vast majority of each of the individual elements, stonin 1 and general transcription factor IIA, 1-like. Alternative splicing results in multiple transcript variants. The significance of these read-through variants and the function of the resulting protein products have not yet been determined. [provided by RefSeq, Oct 2010]